http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Ramadan Awad (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
Graviola leaves extract enhances the anticancer effect of cisplatin on various cancer cell lines
Mai G. Awad,Ramadan A. Ali,Dalia D. Abd El‑Monem,Mohammed A. El‑Magd 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.4
Background Cisplatin (CIS) is widely applied as an anticancer drug for various cancer types, including liver, breast, colorectal, and pancreatic cancers; however, its usage is limited due to side efects. Objective We investigated whether combined therapy of Graviola (Annona muricata) leaves extract (GLE) and CIS could reduce CIS doses without decreasing its anticancer potential. Methods The MCF7, HepG2, CaCo2, or PANC1 cells were divided into four groups for each cell line as follows: group1 (G1): untreated cells, G2: cells treated with GLE, G3: cells treated with CIS, and G4: cells treated with GLE, after 2 h treated with CIS. All combinations were prepared as non-constant ratio from GLE. The cytotoxicity, gene expression, cell cycle arrest were determined by MTT assay, real-time PCR, and cell fow cytometry, respectively. Results Treatment with GLE and/or CIS-induced cytotoxic efect on HepG2, MCF7, CaCo2, and PANC1 cancer cells with the best efect of combined therapy. All twelve non-constant ratio combinations (GLE+CIS) for each cell line resulted in a signifcant higher cytotoxic efect than single drug treatment. The combination index (CI) values for all combinations were less than one, indicating the presence of synergistic cytotoxic efect between CIS and GLE against the four cancer cell lines. This anticancer efect was triggered through mitochondrial-dependent apoptosis with the downregulation of caspase3, Bax, and p53 and upregulation of Bcl2. GLE also shifted G0/G1 phase of cell cycle arrest induced by CIS to S and G2/M phases. Interestingly, this combined therapy did not afect oxidative stress (indicated by higher malondialdehyde level and lower activities of SOD, CAT, and GPX) induced by CIS; however, it downregulated the expression of MAPK1 and multidrug resistance gene MDR1.
-
Structural, Optical and Room Temperature Magnetic Study of Mn-Doped ZnO Nanoparticles
Majed Sharrouf,Ramadan Awad,Salem Marhaba,Douaa El-Said Bakeer 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.4
Undoped and Mn-doped ZnO nanoparticles (Zn1-xMnxO), with nominal weight percentages (0:00 ≤ x ≤ 0.10), have been synthesized by co-precipitation technique. The synthesized nanoparticles are characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), ultraviolet-visible spectroscopy (UV) and Fourier transform infrared spectroscopy (FTIR). From XRD analysis, the compound ZnMnO3 is formed for x ≥ 0.05 with cubic structure (a = 8.3694 Å) and its concentration increases with x. Moreover, XRD analysis reveals the wurtzite hexagonal crystal structure for ZnO. The lattice parameters (a and c) of Zn1-xMnxO are calculated and they increase with the doping concentration of Mn as a consequence of the larger ionic size of Mn2+ ions compared to Zn2+ ions. The crystallite size is calculated for all the samples using Debye– Scherrer's method (SSM), Williamson–Hall methods (UDM, USDM and UDEDM) and SizeStrain Plot method (SSP), and the results are in good agreement with TEM. The presence of functional groups and the chemical bonding is confirmed by FTIR spectra that shows a peak shift between undoped and doped ZnO. The energy bandgap (Eg) is calculated for different concentrations of Mn (0.00 ≤ x ≤ 0.10) by using the UV-visible optical spectroscopy, between 300 nm and 800 nm, showing a noticeable drop in Eg with x. At room temperature, the magnetization of the samples reveals the intrinsic ferromagnetic (FM) behavior of undoped ZnO, ferromagnetic behavior of ZnxMn1-xO (0.01 ≤ x ≤ 0.03) and the co-existence of ferromagnetic and paramagnetic behavior for ZnxMn1-xO (0.05 ≤ x ≤ 0.10). This ferromagnetism is decreased for the doped samples as a consequence of antiferromagnetic coupling between Mn ions. The two samples correspond to x = 0.01 and x = 0.10, tend to be superparamagnetic because of the formation of single domain particles as a consequence of small particle size. x = 0.03 shows an optimum value of Mn concentration for maximum saturation magnetization and the best ferromagnetic nature.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
