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Ex-situ conservation and cytotoxic activity assessment of native medicinal orchid: Coelogyne stricta
Thapa, Bir Bahadur,Thakuri, Laxmi Sen,Joshi, Pusp Raj,Chand, Krishna,Rajbahak, Sabari,Sah, Anil Kumar,Shrestha, Resha,Paudel, Mukti Ram,Park, So Young,Pant, Bijaya The Korean Society of Plant Biotechnology 2020 JOURNAL OF PLANT BIOTECHNOLOGY Vol.47 No.4
Ex-situ conservation of the ornamental and medicinal orchid, Coelogyne stricta, was performed by mass propagation using seed culture. Propagation stages were optimized using full- and half-strength solidified MS medium with different phytohormones. Maximum seed germination (88 ± 0.5% over 6 weeks of culture) was achieved on half-strength MS medium supplemented with 15% coconut water. Maximum shoot numbers were found on full-strength MS medium supplemented with 1 mg/L BAP, 2 mg/L Kinetin, and 10% coconut water, while the longest root was developed on full-strength MS medium with 1.5 mg/L IBA. A 2:1:1 combination of coco-peat, pine bark, and sphagnum moss was found to be a suitable potting mixture resulting in 80% seedling survivability. The cytotoxic activity of extracts of both wild plants and in vitro-developed protocorms was determined using an MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay on a cervical cancer cell line. The wild plant extract inhibited the growth of 41.99% of cells, showing that this extract has moderate cytotoxic activity toward cervical cancer cells.