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Isolation and Biocontrol Potential of Bacillus amyloliquefaciens Y1 against Fungal Plant Pathogens
Jamal, Qaiser,Lee, Yong Seong,Jeon, Hyeon Deok,Park, Yun Suk,Kim, Kil Yong Korean Society of Soil Science and Fertilizer 2015 한국토양비료학회지 Vol.48 No.5
This study was performed to investigate thermophilic bacteria from soil having broad antifungal spectrum against Rhizoctonia solani, Colletotrichum gloeosporioides, Phytophthora capsici, Fusarium oxysporum f.sp. lycopersici, and Botrytis cinerea. One isolate selected could resist heat shock of $60^{\circ}C$ for one hour, and had broad antifungal activity in dual culture assay against all tested fungal pathogens and was identified as Bacillus amyloliquefaciens Y1 using 16S rRNA gene sequence. Further investigation for antifungal activity of bacterial culture filtrate (BCF) and butanol crude extract (BCE) of various concentrations showed broad spectrum antifungal activity and fungal growth inhibition significantly increased with increasing concentration with highest growth inhibition of 100% against R. solani with 50% BCF and 11 mm of zone of inhibition against R. solani with 4 mg BCE concentration. Treatment of butanol crude extract resulted in deformation, lysis or degradation of C. gloeosporioides and P. capsici hyphae. Furthermore, B. amyloliquefaciens Y1 produced volatile compounds inhibiting growth of R. solani (70%), C. gloeosporioides (65%) and P. capsici (65-70%) when tested in volatile assay. The results from the study suggest that B. amyloliquefaciens Y1 could be a biocontrol candidate to control fungal diseases in crops.
Isolation and Biocontrol Potential of Bacillus amyloliquefaciens Y1 against Fungal Plant Pathogens
Qaiser Jamal,Yong Seong Lee,Hyeon Deok Jeon,Yun Suk Park,Kil Yong Kim 한국토양비료학회 2015 한국토양비료학회지 Vol.48 No.5
This study was performed to investigate thermophilic bacteria from soil having broad antifungal spectrum against Rhizoctonia solani, Colletotrichum gloeosporioides, Phytophthora capsici, Fusarium oxysporum f.sp. lycopersici, and Botrytis cinerea. One isolate selected could resist heat shock of 60°C for one hour, and had broad antifungal activity in dual culture assay against all tested fungal pathogens and was identified as Bacillus amyloliquefaciens Y1 using 16S rRNA gene sequence. Further investigation for antifungal activity of bacterial culture filtrate (BCF) and butanol crude extract (BCE) of various concentrations showed broad spectrum antifungal activity and fungal growth inhibition significantly increased with increasing concentration with highest growth inhibition of 100% against R. solani with 50% BCF and 11 mm of zone of inhibition against R. solani with 4 mg BCE concentration. Treatment of butanol crude extract resulted in deformation, lysis or degradation of C. gloeosporioides and P. capsici hyphae. Furthermore, B. amyloliquefaciens Y1 produced volatile compounds inhibiting growth of R. solani (70%), C. gloeosporioides (65%) and P. capsici (65-70%) when tested in volatile assay. The results from the study suggest that B. amyloliquefaciens Y1 could be a biocontrol candidate to control fungal diseases in crops.
조병완,Muhammad Ali Sikandar,Zafar Baloch,Amjad Naseer,Naveed Jan,Qaiser Jamal 한양대학교 세라믹연구소 2018 Journal of Ceramic Processing Research Vol.19 No.2
Silica fume is most commonly used mineral admixture both in high performance concrete (HPC) and self-compacting concrete(SCC). While the incorporation of silica fume in concrete has been extensively investigated in HPC and SCC, furtherresearches are needed to optimize the dosage of silica fume and cement content in self-compacting high performance concrete(SCHPC). This paper presents the results of tests carried out to study the effect of w/b ratio and binder content (cement andsilica fume content) on properties of SCHPC. Eighteen batches of concrete with binder content ranging from 500-600 kg/m3were produced at two w/b ratios (0.23 and 0.25); with silica fume amount varying from 0 to 20 percent by weight of cement. Super Plasticizer (SP) dosage, porosity, water absorption, compressive strength, modulus of elasticity, splitting tensile strength,autogenous shrinkage (AS) and hydration characteristics were evaluated for all SCHPC mixes. The results indicate thatoptimum w/b ratio and binder content (cement and silica fume content) is required to get better performance from SCHPC,as high cement content and silica fume dosage tend to deteriorate the overall performance owing to reduced mechanicalperformances, high degree of hydration and greater autogenous shrinkage in SCHPC mixes incorporating silica fume.