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RISS 인기검색어
- 검색키워드
- 저자 : Pushpa Dhar (검색결과 2 건)
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Arnab Bhattacharya,Pushpa Dhar,Raj D. Mehra 대한해부학회 2012 Anatomy & Cell Biology Vol.45 No.4
The effects of sodium arsenite exposure on the hepatic maturation period of cellular and functional reorganization in developing rat livers were evaluated. Animals received intraperitoneal injections of sodium arsenite (1.5 mg/kg body weight) or distilled water on days 9 to 28 after birth. On day 29, the animals were sacrificed either by cervical dislocation or by perfusion fixation. The perfusion fixed liver tissue was processed for paraffin embedding, sectioning and hematoxylin and eosin staining. The fresh liver tissue was processed for cryo-sectioning followed by Sudan Black B staining and for biochemical estimation of reduced glutathione. Microscopic observation revealed comparable preserved hepatic lobular patterns and distributions of uninucleate and binucleate hepatocytes in the control and the experimental groups. The mean nuclear area and diameter of the hepatocytes was increased in the experimental group. Lipid droplet distribution pattern in Sudan Black B stained sections revealed higher staining intensity towards the centrilobular area in both groups. Semiquantitative estimation of staining intensity showed lower mean gray values in zone 3 than in zones 2 and 1 (suggestive of the setting in of the adult pattern) in both groups. The reduced glutathione levels in the liver tissue and the altered nuclear size of the hepatocytes in the experimental group suggested the impairment of morphological and biochemical processes induced by arsenic exposure during the postnatal period.
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Kamakshi Mehta,Balpreet Kaur,Kamlesh Kumar Pandey,Saroj Kaler,Pushpa Dhar 대한해부학회 2020 Anatomy & Cell Biology Vol.53 No.3
Since, oxidative stress has been suggested as one of the mechanisms underlying arsenic-induced toxicity, the present study focused on the role of antioxidant (curcumin) supplementation on behavioral, biochemical, and morphological alterations with context to mice hippocampus (CA1) following arsenic trioxide (As₂O₃) administration. Healthy male Swiss albino mice were divided into control and experimental groups. As₂O₃ (2 mg/kg bw) alone or along with curcumin (100 mg/kg bw) was administered to experimental groups by oral route for 45 days whereas the control groups received either no treatment or vehicle for curcumin. Animals were subjected to behavioral study towards the end of the experimental period (day 33-45). On day 46, the brain samples were obtained and subjected either to immersion fixation (for morphometric observations) or used afresh for biochemical test. Behavioral tests (open field, elevated plus maze, and Morris water maze) revealed enhanced anxiety levels and impairment of cognitive functions in As₂O₃ alone treated groups whereas a trend of recovery was evident in mice simultaneously treated with As₂O₃ and curcumin. Morphological observations showed noticeable reduction in stratum pyramidale thickness (CA1), along with decrease in density and size of pyramidal neurons in As₂O₃ alone exposed group as compared to As₂O₃+Cu co-treated group. Hippocampal glutathione levels were found to be downregulated in animals receiving As₂O₃ as against the levels of controls and curcumin supplemented animals, thereby, suggestive of beneficial role of curcumin on As₂O₃ induced adverse effects.
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