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Devi, Prattipati Subhashini,Satyanarayana, Botcha,Arundhati, Atluru,Rao, Tamanam Raghava 한국작물학회 2014 Journal of crop science and biotechnology Vol.17 No.1
The present study is an attempt to prepare synthetic seeds using alginate encapsulation of nodal explants of Sterculia urens. Regeneration potential of encapsulated nodal segments was tested on MS basal, MS + BA ($3.0mg\;L^{-1}$), MS + TDZ ($0.2mg\;L^{-1}$), and MS + BA ($0.5mg\;L^{-1}$) + NAA ($2.0mg\;L^{-1}$). The regeneration potential was maximum ($73.33{\pm}1.33$) on MS + TDZ ($0.2mg\;L^{-1}$) followed by ($69.33{\pm}1.76$) on MS + BA ($3mg\;L^{-1}$) even after 6 months of storage at $4^{\circ}C$, whereas controls did not show any regeneration potential after 1 week. These findings suggest synthetic seed technology as an alternative method for micropropagation and germplasm conservation of Sterculia urens an endangered species.
Prattipati Subhashini Devi,Botcha Satyanarayana,Atluru Arundhati,Tamanam Raghava Rao 한국작물학회 2014 Journal of crop science and biotechnology Vol.17 No.1
The present study is an attempt to prepare synthetic seeds using alginate encapsulation of nodal explants of Sterculia urens. Regeneration potential of encapsulated nodal segments was tested on MS basal, MS + BA (3.0 mg L-1), MS + TDZ (0.2 mg L-1), andMS + BA (0.5 mg L-1) + NAA (2.0 mg L-1). The regeneration potential was maximum (73.33 ± 1.33) on MS + TDZ (0.2 mg L-1) followedby (69.33 ± 1.76) on MS + BA (3 mg L-1) even after 6 months of storage at 4°C, whereas controls did not show any regenerationpotential after 1 week. These findings suggest synthetic seed technology as an alternative method for micropropagation andgermplasm conservation of Sterculia urens an endangered species.