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Fernandez, Maria Elena,Goszczynski, Daniel Estanislao,Prando, Alberto Jose,Peral-Garcia, Pilar,Baldo, Andres,Giovambattista, Guillermo,Liron, Juan Pedro Korean Society of Animal Sciences and Technology 2014 한국축산학회지 Vol.56 No.5
Puberty is a stage of sexual development determined by the interaction of many loci and environmental factors. Identification of genes contributing to genetic variation in this character can assist with selection for early pubertal bulls, improving genetic progress in livestock breeding. Thyroid hormones play an important role in sexual development and spermatogenic function. The objective of this study was to evaluate the association between single nucleotide polymorphisms (SNPs) located in thyroglobulin(TG) gene with age of puberty in Angus bulls. Four SNPs were genotyped in 273 animals using SEQUENOM technology and the association between markers and puberty age was analyzed. Results showed a significant association (P < 0.05) between these markers and puberty age estimated at a sperm concentration of 50 million and a progressive motility of 10%. This is the first report of an association of TG polymorphisms with age of puberty in bulls, and results suggest the importance of thyroidal regulation in bovine sexual development and arrival to puberty.
Ingrid R. Marques,Guilherme Zin,Lidia T. Prando,Cristiana C. Bretanha,Mariane C. Proner,Elisandra Rigo,Katia Rezzadori,Cristiane da Costa,Marco Di Luccio 한국고분자학회 2020 Macromolecular Research Vol.28 No.12
Modification of the characteristics of polymeric membranes is regarded as an alternative to control the flux decline caused by the concentration polarization and the fouling in ultrafiltration (UF). This work investigated the use of dopamine (DA) and polyethyleneimine (PEI) to hydrophilize the surface and to improve the performance of a 50 kDa polyethersulfone UF membrane. The hydraulic permeance of membranes was investigated for the membranes modified using a DA solution with its concentration fixed at 2 mg·mL-1 and a PEI solution with its concentration varying from 0 to 16 mg·mL-1. The membrane modified in a 16 mg·mL-1 solution of PEI presented the uppermost hydraulic permeance (33 L·h-1·m-2). This membrane and two other controls (unmodified and modified membranes with a 2 mg·mL-1 solution of DA) were further characterized for surface chemistry using Fourier transform infrared by attenuated reflectance (FTIR-ATR) and X-ray excited photoelectron spectroscopy (XPS) charge (zeta potential), morphology (field emission scanning electron microscopy (FESEM) and optical interferometry), water mass gain, and performance in bovine serum albumin (BSA) UF. XPS showed an increase in the nitrogen content and a decrease in the sulfur content indicating the membrane modification. Membrane modification with DA/PEI caused an increase in hydrophilicity, which could reduce the interaction of BSA with the membrane surface. The flux of protein solutions was improved by 400%, and there was a complete recovery of the water flux after chemical cleaning of the modified membrane.
( Maria Elena Fernandez ),( Daniel Estanislao Goszczynski ),( Alberto Jose Prando ),( Pilar Peral Garcia ),( Andres Baldo ),( Guillermo Giovambattista ),( Juan Pedro Liron ) 한국동물자원과학회(구 한국축산학회) 2014 한국축산학회지 Vol.56 No.17
Puberty is a stage of sexual development determined by the interaction of many loci and environmental factors. Identification of genes contributing to genetic variation in this character can assist with selection for early pubertalbulls, improving genetic progress in livestock breeding. Thyroid hormones play an important role in sexualdevelopment and spermatogenic function. The objective of this study was to evaluate the association betweensingle nucleotide polymorphisms (SNPs) located in thyroglobulin(TG) gene with age of puberty in Angus bulls. FourSNPs were genotyped in 273 animals using SEQUENOM technology and the association between markers andpuberty age was analyzed. Results showed a significant association (P < 0.05) between these markers and pubertyage estimated at a sperm concentration of 50 million and a progressive motility of 10%. This is the first report of anassociation of TG polymorphisms with age of puberty in bulls, and results suggest the importance of thyroidalregulation in bovine sexual development and arrival to puberty.