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RISS 인기검색어
- 검색키워드
- 저자 : Phillip C . Wankat ( Yoon Mo Koo (검색결과 2 건)
- 무료
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- 유료
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Molecular Structure of Dextran Gel
구윤모,Koo, Yoon-Mo,Wankat, Phillip C. 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.1
Sephadex 젤의 주 구성원인 dextran은 주로 1-6 glucosidic 결합에 의해 연결된 단위체, ${\alpha}$-D-glucopyranose로 이루어진 선형 homopolymer (polysaccharide)이다. dextran의 기본구조를 이루는 ${\alpha}$-gentiobiose (6-0-${\alpha}$-glucopyranosyl-${\alpha}$-D-glucopyranose)의 conformation을 consistent force field 방법을 사용하여 연구하였다. 기준좌표계에 있어서 각 원자들의 위치를 나타내는 방법을 제시하였다. Sephadex 젤의 분자구조를 두개의 원주로써 모사하였는데, 하나는 ${\alpha}$-gentiobiose, 다른 하나는 cross-linking bridge를 나타내었다. dextran 젤 (sephadex G-25)의 구조적 크기를 이들 원주의 반지름과 길이로써 표시하였는데, ${\alpha}$-gentiobiose는 반지름 2.54 $\dot{A}$ 길이 5.61 $\dot{A}$, cross-linking bridge는 반지름 1.57 $\dot{A}$, 길이 4.84 $\dot{A}$ 이었다. Dextran, a main constituent of Sephadex gel, is a linear homopolymer (polysaccharide) composed almost exclusively of the monomeric unit, ${\alpha}$-D-glucopyranose, linked mainly by 1-6 glucosidic bonds. As a basic building block of dextran, the conformation of ${\alpha}$-gentiobiose (6-O-${\alpha}$-D-glucopyranosyl-${\alpha}$-D-glucopyranose) in vacuum was studied by the technique of the consistent force field. We presented the procedures of identifying the position of each atoms in a reference coordinate system. The molecular structure of a Sephadex gel was simulated as two sets of cylinders: one for a unit of ${\alpha}$-gentiobiose and the other for a cross-linking bridge. The structural dimension of a dextran gel (Sephadex G-25) was expressed by the radius and the length of those cylinders: a cylinder with a radius at 2.54 $\dot{A}$ and a length of 5.61 $\dot{A}$ for ${\alpha$}-gentiobiose and a cylinder with a radius of 1.57 $\dot{A}$ a length of 4.84 $\dot{A}$ for the cross-linking bridge.
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구윤모,Phillip C . Wankat ( Yoon Mo Koo,Phillip C . Wankat ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.1
Dextran, a main constituent of Sephadex gel, is a linear homopolymer (polysaccharide) composed almost exclusively of the monomeric unit, α-D-glucopyranose, linked mainly by 1-6 glucosidic bonds. As a basic building block of dextran, the conformation of α-gentiobiose (6-0-α-D-glucopyranosyl-α-D-glucopyranose) in vacuum was studied by the technique of the consistent force field. We presented the procedures of identifying the position of each atoms in a reference coordinate system. The molecular structure of a Sephadex gel was simulated as two sets of cylinders: one for a unit of α-gentiobiose and the other for a cross-linking bridge. The structural dimension of a dextran gel (Sephadex G-25) was expressed by the radius and the length of those cylinders: a cylinder with a radius at 2.54 Å and a length of 5.61 Å for α-gentiobiose and a cylinder with a radius of 1.57 Å a length of 4.84 Å for the cross-linking bridge.
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