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        Ovicidal, larvicidal and oviposition repelling action of a nanoemulsion of citronella essential oil (Cymbopogon winterianus) on Cochliomyia hominivorax (Diptera: Calliphoridae)

        Bricarello Patrizia Ana,de Barros Giuliano Pereira,Seugling Jaqueline,Podestá Raíssa,Velerinho Maria Beatriz,Mazzarino Letícia 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

        Cochliomyia hominivorax is the dipteran that causes the worst and most serious types of myiasis in animals and humans in the Americas. They are parasitic affections caused by the massive infestation of larvae of this species on the tissues of living mammals. The routine treatment uses chemosynthetic products that have high toxicity and can cause several injuries to animal and human health, besides environmental contamination. The devel opment of more friendly alternatives to the treatment of this disease is an important knowledge gap nowadays. Citronella (Cymbopogon winterianus) is the most widely cultivated medicinal plant in tropical and subtropical regions. Essential oils have a recognised repellent and larvicidal activity against a wide variety of insects. This work aimed to evaluate the repellent, ovicidal and larvicidal efficacy of a topical commercial formulation composed of 15% (w/v) nanoemulsified Citronella essencial oil (Nano Citronela Plus®) in in vitro trials on specimens of C. hominivorax created under laboratory conditions. Nano Citronela Plus® is an oil-in-water nanoemulsion obtained by high-pressure homogenization method. The composition of tested nanoemulsion was Cymbopogon winterianus essential oil, vanillin, caprylic/capric triglyceride, polysorbate 80, sorbitan oleate, tocopheryl acetate, sodium benzoate and water. Nano Citronela Plus® demonstrated 100% lethality on eggs and first and second stage larvae of C. hominivorax. Mortality in the larvae of third stage was 69.33% and inhibited the oviposition of C. hominivorax females. Nano Citronela Plus® proved to be a possible herbal alternative for the control and treatment of myiasis caused by C. hominivorax. In vivo studies may test this hypothesis.

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        Leukocyte platelet-rich fibrin in endodontic microsurgery: a report of 2 cases

        Pires Mariana Domingos,Martins Jorge N. R.,Baruwa Abayomi Omokeji,Pereira Beatriz,Ginjeira António 대한치과보존학회 2022 Restorative Dentistry & Endodontics Vol.47 No.2

        Endodontic microsurgery is a predictable treatment option when orthograde treatment or retreatment is unsuccessful or unfeasible. However, when there is a gross compromise of periapical bone, achievement of bone regeneration after the surgical procedure may be hampered. In such cases, the application of guided tissue regeneration principles, with adjunctive use of leukocyte platelet-rich fibrin to fill the bone defect as a bone substitute and as a membrane to cover the site, provides a cost-effective solution with the benefits of accelerated physiological healing and reduced post-surgical pain and discomfort. This case report presents 2 cases of endodontic microsurgery of the upper lateral incisors with loss of buccal cortical plate, where platelet-rich fibrin was successfully applied.

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