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Insecticidal activity of five Chinese medicinal plants against Plutella xylostella L. larvae
Patcharaporn Vanichpakorn,Xiao-Xi Cen,Wei Ding 한국응용곤충학회 2010 Journal of Asia-Pacific Entomology Vol.13 No.3
Twenty-five extracts of five Chinese medicinal plant species were screened for insecticidal activity against the diamondback moth, Plutella xylostella L., larvae by a leaf dipping bioassay method. The ethyl acetate,ethanol, and acetone extracts of Veratrum nigrum L. root and rhizome, the ethyl acetate extract of Phytolacca americana L. root, and the petroleum ether extract of Pseudolarix kaempferi Gord. root bark were effective against P. xylostella. Among them, the ethyl acetate extract of V. nigrum showed the strongest insecticidal activity against the second and third instar larvae of P. xylostella, with LC50 values of 225 and 335 ppm,respectively. The other extracts gave little or no insecticidal activity against P. xylostella. These results indicated that V. nigrum may be a promising naturally occurring agent for P. xylostella larval control, and that the organic solvent used for plant extraction can affect its insecticidal activity.
Jie Wei,Wei Ding,Yan-Guo Zhao,Patcharaporn Vanichpakorn 한국응용곤충학회 2011 Journal of Asia-Pacific Entomology Vol.14 No.3
Four different extracts of Aloe vera L. leaves were evaluated for acaricidal activity against female adults of carmine spider mite, Tetranychus cinnabarinus (Boisduval), by slide-dip bioassay. At 72 h after treatment, the acetone extract showed the strongest acaricidal activity with LC_50 value of 90 ppm. The LC_50 values for ethyl acetate, water, and ethanol extracts were 113, 340, and 391 ppm, respectively. The acetone extract was fractionated using a silica gel column. Among the twenty-two fractions obtained the fifth, tenth, eleventh,twelfth, fifteenth, and seventeenth fractions showed strong acaricidal activity, causing 80.39 to 92.16%mortality at 72 h after treatment. The tenth and eleventh fractions had the strong activity, with LC_50 values of 44 ppm and 33 ppm, respectively. The results suggested that A. vera has a great potential for development as a botanical acaricide for T. cinnabarinus control.