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Particle Growth in Oxalate Process I
Park, Zee-Hoon,Shin, Hyo-Soon,Lee, Byung-Kyo The Korean Ceramic Society 1996 The Korean journal of ceramics Vol.2 No.2
Barium titanyl oxalates, strontium titanyl oxalates and calcium zirconyl oxalates were prepared with variation of solution concentration and method of adding mixed metal ion solution into oxalic acid. Then they were aged in distilled water, ethanol or methanol, respectively. Barium titanyl oxalates and calcium zironyl oxalates were grown in water and strontium titanyl oxalates were groun in both water and methanol. They were supposed to be grown through the solutionl and reprecipitation mechanism. Nonuniform dispersion of particles in liquid phase is thought to cause abnormal particle growth.
Park, Jong-Hyuk,Kwak, Jong-Hwan,Khoo, Ja-Heouk,Park, Sung-Hoon,Kim, Dae-Up,Ha, Dong-Mun,Choi, Sang-Un,Kang, Se-Chan,Zee, Ok-Pyo 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.8
Cytotoxicity-guided fractionation and separation of MeOH extract from Androsace umbellata (Lour.) Merr. led to the isolation of four triterpenoid saponins. Compounds isolated from the n-BuOH soluble fraction were identified as saxifragifolin C (1), A (2), B (3), and D (4) by spectroscopic analysis. Antiproliferative effect of isolated compounds were evaluated by the sulforhodamin B assay against multidrug resistance (MDR; MES-SA/DX5 and HCT15/CLO2) and non-MDR (A549, SK-OV-3, SK-MEL-2, MES-SA, and HCT15) human tumor cell lines. All compounds exhibited strong cytotoxicity against non-MDR human tumor cell lines with $IC_{50}$ values of 0.19-2.37 ${\mu}M$. MDR cells and non-MDR cells had similar sensitivity to these compounds, however, MDR cells were highly resistant to doxorubicin. Compounds 1-4 induced an increase in the percentage of Annexin V-binding cells, indicating that 1-4 induced apoptosis in RAW 264.7 cells. Also, the condensation of nuclei, a characteristic morphological change of apoptosis, was observed in RAW 264.7 cells by the treatment with n-BuOH fraction, compounds 3 and 4, respectively.
Zhang, Tiejun,Li, Yuwen,Park, Kyeong Ah,Byun, Hee Sun,Won, Minho,Jeon, Juhee,Lee, Yoonjung,Seok, Jeong Ho,Choi, Seung-Won,Lee, Sang-Hee,Man Kim, Jin,Lee, Ji Hoon,Son, Chang Gue,Lee, Zee-Won,Shen, Han- Landes Bioscience 2012 AUTOPHAGY Vol.8 No.4
<P>Targeted disruption of STAT3 function has proven to be a useful cancer therapeutic approach by inducing apoptotic cell death. Cucurbitacin is currently under development as a small molecule of STAT3 inhibitor to trigger cell death in many cancers. Here, we systematically studied the molecular mechanisms underlying cucurbitacin-induced cell death, in particular the involvement of autophagy. Treatment with cucurbitacin resulted in non-apoptotic cell death in a caspase-independent manner. Notably, cucurbitacin enhanced excessive conversion of lipidated LC3 (LC3-II) and accumulation of autophagosomes in many cell types. Such autophagy and cell death induced by cucurbitacin were independent of its ability to inhibit STAT3 function, but mainly mediated by enhanced production of mitochondrial-derived reactive oxygen species (ROS), and subsequently activation of extracellular signal-regulated kinase (ERK) and c-jun NH2-terminal kinase (JNK). Interestingly, both the autophagy inhibitor wortmannin and knockdown of Atg5 or Beclin 1 failed to rescue the cells from cucurbitacin-induced cell death, as suppression of autophagy induced the mode of cell death to shift from autophagic cell death to caspase-dependent apoptosis. Thus the present study provides new insights into the molecular mechanisms underlying cucurbitacin-mediated cell death and supports cucurbitacin as a potential anti-cancer drug through modulating the balance between autophagic and apoptotic modes of cell death.</P>
SNX14 is a bifunctional negative regulator for neuronal 5‐HT<sub>6</sub> receptor signaling
Ha, Chang Man,Park, Daehun,Kim, Yoonju,Na, Myeongsu,Panda, Surabhi,Won, Sehoon,Kim, Hyun,Ryu, Hoon,Park, Zee Yong,Rasenick, Mark M.,Chang, Sunghoe The Company of Biologists Limited 2015 Journal of cell science Vol.128 No.9
<P>The 5-hydroxytryptamine (5-HT, also known as serotonin) subtype 6 receptor (5-HT6R, also known as HTR6) plays roles in cognition, anxiety and learning and memory disorders, yet new details concerning its regulation remain poorly understood. In this study, we found that 5-HT6R directly interacted with SNX14 and that this interaction dramatically increased internalization and degradation of 5-HT6R. Knockdown of endogenous SNX14 had the opposite effect. SNX14 is highly expressed in the brain and contains a putative regulator of G-protein signaling (RGS) domain. Although its RGS domain was found to be non-functional as a GTPase activator for Gas, we found that it specifically bound to and sequestered Gas, thus inhibiting downstream cAMP production. We further found that protein kinase A (PKA)-mediated phosphorylation of SNX14 inhibited its binding to Gas and diverted SNX14 from Gas binding to 5-HT6R binding, thus facilitating the endocytic degradation of the receptor. Therefore, our results suggest that SNX14 is a dual endogenous negative regulator in 5-HT6R-mediated signaling pathway, modulating both signaling and trafficking of 5-HT6R.</P>
Seo, Ji Hae,Cha, Jong-Ho,Park, Ji-Hyeon,Jeong, Chul-Ho,Park, Zee-Yong,Lee, Hye-Suk,Oh, Seung Hyun,Kang, Ju-Hee,Suh, Se Won,Kim, Kyoung Hoon,Ha, Jun Yong,Han, Sang Hee,Kim, Se-Hee,Lee, Ji-Won,Park, Jeo American Association for Cancer Research 2010 Cancer Research Vol.70 No.11
<P>The N-acetyltransferase arrest defective 1 (ARD1) is an important regulator of cell growth and differentiation that has emerged recently as a critical molecule in cancer progression. However, the regulation of the enzymatic and biological activities of human ARD1 (hARD1) in cancer is presently poorly understood. Here, we report that hARD1 undergoes autoacetylation and that this modification is essential for its functional activation. Using liquid chromatography-tandem mass spectrometry and site-directed mutational analyses, we identified K136 residue as an autoacetylation target site. K136R mutation abolished the ability of hARD1 to promote cancer cell growth in vitro and tumor xenograft growth in vivo. Mechanistic investigations revealed that hARD1 autoacetylation stimulated cyclin D1 expression through activation of the transcription factors beta-catenin and activator protein-1. Our results show that hARD1 autoacetylation is critical for its activation and its ability to stimulate cancer cell proliferation and tumorigenesis.</P>
Cytotoxic Constituents of Sorbaria sorbifolia var. stellipila
Kim, Dae-Keun,Choi, Sang-Hoon,Lee, Jung-Ock,Ryu, Shi-Yong,Park, Dae-Kyu,Shin, Dae-Hee,Jung, Jee-Hyung,Pyo, Suhk-Keung,Lee, Kang-Ro,Zee, Ok-Pyo The Pharmaceutical Society of Korea 1997 Archives of Pharmacal Research Vol.20 No.1
The activity-guided fractionation upon the MeOH extract of the aerial parts of Sorbaria sorbifolia var. stellipila led to the isolation of two cucurbitacin-compounds, cucurbitacin D and cucurbitacin F, as active principles. Two compounds were shown to exhibit significant cytotoxicity against cultured human tumor cell lines, A-549, SK-OV-3, SK-MEL-2, XF-498, and HCT 15.
Byung-Seon Choi,Geun-Il Park,Seong-Hoon Kim,Juhyeon Yoon,Yoon-Yeong Bae,Sung_Kyun Zee,Ho-Yeon Yang,Seung-Kon Ryu 한국방사성폐기물학회 2003 방사성폐기물학회지 Vol.1 No.1
원자력시설에서 방사성요오드 제거용으로 사용되는 TEDA 첨착활성탄의 고온공정에서치 메틸요오드의 제거성능을 은이온제올라이트(AgX)와 상호 비교하였다. 3-40 온도범위에서 온도에 따른 메틸요오드의 흡착량 및 탈착후 잔존량을 측정한 결과, 비첨착활성탄의 흡착성능은 온도가 증가함에 따라 급격히 감소하지만 TEDA 첨착활성탄의 흡착성능은 10 부근에서도 AgX-10과 거의 유사한 값을 나타내었고, 탈착후 잔존량은 25 까지도 비첨착활성탄에 비하여 매우 높은 값을 유지하였다. 또한 10 이상의 고온공정에서 AgX 및 TEDA 첨착활성탄을 충전한 고정층 파과특성을 상호 비교한 결과 10 이상에서 AgX-10의 메틸요오드 흡착량 및 잔존량은 TEDA 첨착활성탄에 비하여 평균 30%정도 높은 값을 나타내어 고온에서 더 흡착성능이 우수함을 보여주고 있다. 흡착반응 후 생성된 기체의 성분을 분석한 결과를 토대로 AgX-10 흡착제를 충전한 고정층에서 메틸요오드 제거 메카니즘을 제안하였다. Adsorption and desorption characteristics of methyl iodide at high temperature conditions up to 25 by TEDA-impregnated activated carbon and silver-ion exchanged zeolite(AgX-10), which are used for radioiodine retention in nuclear facility, were experimentally evaluated. In the range of temperature from 3 to 25, the adsorption capacity of base activated carbon decreased sharply with increasing temperature but that of TEDA-impregnated activated carbon showed higher value even at high temperature ranges. Especially, the residual amount of methyl iodide after desorption on TEDA-AC represented 30% lower value than that on AgX-10. However, it can be used as an adsorbent for the removal of methyl iodide up to 15 if it is preventing explosion by Ignition. The breakthrough curves of methyl iodide in the fixed bed packed with AgX-10 uP to 40 were compared upon the effects of bed temperatures, bed depth and input concentration of methyl iodide. Removal mechanism of methyl iodide on AgX-10 was proposed, based on the analysis of by-product gas generated from adsorption reaction.