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RISS 인기검색어
- 검색키워드
- 저자 : Panuwan Chantawannakul (검색결과 3 건)
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Diapause hormone terminates larval diapause in the bamboo borer, Omphisa fuscidentalis (Hampson)
Phakamas Subta,Suphawan Suang,Panuwan Chantawannakul,Manaporn Manaboon 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.3
The larvae of the bamboo borer (Omphisa fuscidentalis Hampson) maintain a diapause state for approximately nine months. However, changes in the ecdysteroid titer lead to the termination of larval diapause when juvenile hormone analogue (JHA) is applied. The hormonal mechanisms that terminate larval diapause are still unknown. Recently, it was found that the O. fuscidentalis diapause hormone and pheromone biosynthesis activating neuropeptide genes (Ompfu-DH-PBAN) were expressed during the development of both larvae and pupae and during the late larval diapause. This result suggested that diapause hormone (DH) might be involved in the termination of larval diapause. Thus, our study aims to determine the effect of DH on diapause termination in the bamboo borer. The response of diapausing larvae to DH was assayed using the synthesized O. fuscidentalis diapause hormone (Ompfu-DH). After injection with different concentrations of DH (25, 50 and 100 ng/larvae), nearly half of the individual larvae (approximately 45%) became completed or incompleted pupae within 30 days. The mean days of pupation in larvae injected with 25, 50 and 100 ng DH were 26.81 ± 17.86, 26.30 ± 16.45 and 25.30 ± 16.12, respectively. The hemolymph ecdysteroid titer in Ompfu-DH injected-larvae was significantly higher 10–15 days after Ompfu-DH injection and reached a maximum before the formation of the pupal cuticle. These results indicate that Ompfu-DH terminates larval diapause in O. fuscidentalis by increasing the ecdysone in hemolymph, reflecting a new role of DH in the regulation of larval diapause in this species.
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Khacha-ananda, Supakit,Tragoolpua, Khajornsak,Chantawannakul, Panuwan,Tragoolpua, Yingmanee Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11
Antioxidant activity, total phenolic, total flavonoid compounds and cytotoxicity to cancer cell lines of propolis extracts from two extraction methods were investigated in this study. Propolis was collected from Phayao province and extracted with 70% ethanol using maceration and sonication techniques. The antioxidant activity was evaluated by DPPH assay. Total phenolic and flavonoid compounds were also determined. Moreover, the cytotoxicity of propolis was evaluated using MTT assay. The percentage propolis yield after extraction using maceration (18.1%) was higher than using sonication (15.7%). Nevertheless, antioxidant and flavonoid compounds of the sonication propolis extract were significant greater than using maceration. Propolis extract from sonication showed antioxidant activity by $3.30{\pm}0.15$ mg gallic acid equivalents/g extract. Total phenolic compound was $18.3{\pm}3.30$ mg gallic acid equivalents/g extract and flavonoid compound was $20.49{\pm}0.62$ mg quercetin/g extract. Additionally, propolis extracts from two extraction methods demonstrated the inhibitory effect on proliferation of A549 and HeLa cancer cell lines at 24, 48 and 72 hours in a dose-dependent manner. These results are of interest for the selection of the most appropriate method for preparation of propolis extracts as potential antioxidant and anticancer agents.
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Responses of Varroa-resistant honey bees (Apis mellifera L.) to Deformed wing virus
Kitiphong Khongphinitbunjong,Lilia I. de Guzman,Thomas E. Rinderer,Matthew R. Tarver,Amanda M. Frake,Yanping Chen,Panuwan Chantawannakul 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.4
The negative impact of Deformedwing virus (DWV) on European honey bees Apis mellifera is magnified by Varroa destructor parasitism. This study compared the responses of two Varroa-resistant honey bee stocks, pure Russian honey bees (RHB) and out-crossed Varroa Sensitive Hygienic bees, Pol-line (POL) to DWV infection to that of Varroa- susceptible stock, Italian honey bees (IHB). Two-day-old larvae were fed with DWV lysate in different concentrations: undiluted DWV lysate (D1), D1:100, and D1:1000. The unfed larvae served as negative control. Combs containing test larvae were exposed to a common environment during their development using host colonies. Our results showed that only POL displayed variation in DWV levels when fed different DWV concentrations. POL fed highest concentration of DWV inoculum had the highest increase in DWV level than those fed low concentrations and unfed POL. This high increase in DWV level probably contributed to the decrease in the survival and median longevity (LT50) of D1-fed POL. Weights of newly eclosed D1-fed POL were similar to those of the two controls and DWV-fed bees. However, within IHB, D1-fed bees showed significant reductions in weight, days of survival and LT50. Regardless of the concentrations of DWV inoculum, the DWV levels were similarly low within RHB; adult bees had similar weights. Overall, larvae fed D1 had the highest rate of wing deformation. POL and RHB had numerically lower proportions of bees with deformed wings. This study suggests that RHB showed some degree of resistant to DWV as shown by no reduction on weight and numerically lower proportion of wing deformity when compared with the other bee stocks.
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