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- 저자 : Olivo-Marin, Jean-Christophe (검색결과 1 건)
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Quantitative four-dimensional tracking of cytoplasmic and nuclear HIV-1 complexes
Arhel, Nathalie,Genovesio, Auguste,Kim, Kyeong-Ae,Miko, Sarah,Perret, Emmanuelle,Olivo-Marin, Jean-Christophe,Shorte, Spencer,Charneau, Pierre Nature Publishing Group 2006 Nature methods Vol.3 No.10
Emerging real-time techniques for imaging viral infections provide powerful tools for understanding the dynamics of virus-host cell interactions. Here we labeled human immunodeficiency virus-1 (HIV-1) integrase with a small tetracysteine tag, which preserved the virus' infectivity while allowing it to be labeled with the bis-arsenical fluorescein derivative FlAsH. This labeling allowed us to image both intracytoplasmic and intranuclear HIV-1 complexes in three dimensions over time (4D) in human cells and enabled us to analyze HIV-1 kinetics by automated 4D quantitative particle tracking. In the cytoplasm, HIV-1 complexes underwent directed movements toward the nuclear compartment, kinetically characteristic of both microtubule- and actin-dependent transport. The complexes then adopted smaller movements in a very confined volume once associated with the nuclear membrane and more diffuse movements once inside the nucleus. This work contributes new insight into the various movements of HIV-1 complexes within infected cells and provides a useful tool for the study of virus-host cell interactions during infection.
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