Effect of daflon-500®, a flavonoid compound on chlorpyriphos-induced oxidative changes in the hypophysis and testes in adult male rats

Olatunji Aishat O.,Ayo Joseph O.,Suleiman Mohammed M.,Ambali Suleiman F.,Shittu Muftau,Akorede Ganiu J.,Raji Lukman O.,Atata Jamila A.,Biobaku Khalid T.,Azeez Mistura O. 한국독성학회 2022 Toxicological Research Vol.38 No.3

Alteration of redox status is one of the molecular pathways commonly associated with pesticide toxicity. Antioxidants, including those obtained from plant phenolics, have been shown to mitigate pesticide-induced cellular injury. The present study was aimed at evaluating the effect of daflon-500®, a flavonoid compound on sub-chronic chlorpyriphos-evoked changes in antioxidant and biochemical parameters in the hypophysis and testes of adult male rats. Twenty-five male albino rats were randomly divided into 5 groups of 5 animals each. Group I (DW) received distilled water (2 ml/kg); group II (SO) was dosed with soya oil (2 ml/kg); Group III (DAF) received daflon-500® at 1000 mg/kg ̴ 1/5th of LD50 (≥ 5000 mg/kg); group IV (CP) was administered chlorpyriphos at 7.74 mg/kg ̴ 1/10th of LD50 (77.4 mg/kg) while group V (DAF + CP) was previously treated with daflon-500® (1000 mg/kg) and then exposed to CP (7.74 mg/kg), 30 min later. Daily oral regimen administration was done for 60 days after which the animals were sacrificed by cervical venesection after light chloroform anesthesia. The hypophysis and testicular tissues were harvested, and their homogenates were analyzed for malondialdehyde, catalase and superoxide dismutase, and acetylcholinesterase levels. A significant increase in the hypophysis and testicular MDA concentrations, coupled with a decrease in the SOD, CAT, and AChE activities were observed in the CP group. The levels of these oxidative and biochemical parameters were alleviated in the group pretreated with Daflon-500®. Results of this study demonstrated that pre-treatment with Daflon-500® mitigated CP-induced alterations in oxidative and biochemical parameters apparently due to the antioxidant effect of the flavonoid compound.

Performance, hemato-biochemical indices and oxidative stress markers of broiler chicken fed phytogenic during heat stress condition

Olatunji Abubakar Jimoh,Olajumoke Temidayo Daramola,Hafsat Ololade Okin-Aminu,Olayinka Abosede Ojo 한국축산학회 2022 한국축산학회지 Vol.64 No.5

Thermal stress is a tremendous health predicament encountered by poultry farmers withadverse effects on the performance, product stature, health condition, survival, and overallwelfare of poultry birds, and so requires urgent dietary user-friendly strategy to curb. Thisstudy was conducted with 200-day old broilers for the purpose of investigating the potentialof phytogenics in refining the negative effects of heat stress on broiler chicken. Moringa,Phyllanthus and mistletoe leaves were processed as phytogenic supplements and incorporatedinto standard ration for broilers as treatments B1 (control), B2, B3 and B4 diet duringthe peak of thermal discomfort in humid tropics. Growth and carcass indices were monitoredin a 49-day trial and blood samples were harvested at the end of the ordeal period to assesshaematology, serum biochemical and oxidative stress markers with the use of standard procedures. The results obtained showed that the prevailing environmental condition in the studysite indicated that the birds were exposed to heat stress. Birds fed on moringa and mistletoesupplements had higher performance index than birds without supplementation during heatstress condition, while birds fed on mistletoe supplement had the highest survival rate acrossthe treatments. The liveweight, slaughter weight, dressed weight and eviscerated weight ofheat stressed birds fed on moringa, phyllanthus and mistletoe supplements were significantlyhigher than birds on control treatment. Heterophyl/lymphocyte ratio of heat stressed birdswithout supplement were higher than birds on phytogenic supplements, with least valuesrecorded in phyllanthus and mistletoe fed birds. Birds on phytogenic supplement tend tohave lower cholesterol profile, lipid peroxidation and better antioxidant profile than birds oncontrol treatment during heat stress conditions. Mistletoe supplementation in broiler rationenhances the survival rate, as well as promotes growth indices better among the phytogenicsupplements. However, phytogenic supplements did ameliorate the negative effects of thermal discomfort on performance, physiological and oxidative stress in heat-stressed broilerchicken.

Potential of watermelon (Citrullus lanatus) to maintain oxidative stability of rooster semen for artificial insemination

( Olatunji Abubakar Jimoh ),( Micheal Olawale Akinola ),( Bolaji Fatai Oyeyemi ),( Wahab Adekunle Oyeyemi ),( Simeon Olugbenga Ayodele ),( Idowu Samuel Omoniyi ),( Hafsat Ololade Okin-aminu ) 한국축산학회(구 한국동물자원과학회) 2021 한국축산학회지 Vol.63 No.1

Fruits with antioxidant enrichment can be an economically affordable supplement for mitigating oxidative damage prone spermatozoa membrane pathologies. Computer-assisted sperm analyzer and oxidative status were utilized to evaluate the impact of watermelon (Citrullus lanatus) fortification of dextrose saline as diluent for rooster semen and fertility response of hens inseminated. Watermelon juice and dextrose saline were used to formulate diluent of 7 treatments consisting of unextended semen (positive control), 10%, 20%, 30%, 40%, 50% and only dextrose saline (negative control) designated as Treatments 1-7. Pooled semen was obtained from fertile roosters and equilibrated with diluents at ratio 1:2 in the various treatments and were evaluated using computer software coupled microscope and seminal oxidative status assay. 168 laying hens randomly divided into 7 treatment of 8 replicates and 3 hen per replicate. Hen were everted, and semen (2 × 10⁸ Spermatozoa) deposited intra-vagina and eggs collected over 8 weeks to assess fertility and hatchability of eggs laid. The result obtained revealed that watermelon-dextrose saline rooster semen diluent enhanced progressive motility, sperm kinetics and lowered non-progressive motility in T2-T6 compared to T7 over the 3 hours of evaluation. Watermelon addition to rooster semen diluent enhance the antioxidant capacity of rooster semen and lowered lipid peroxide generation. The percentage fertility was highest in T3 (81.01%) and T4 (81.24%) with lowest value obtained in T7 (73.46%). The hatchability of eggs set of hens inseminated with undiluted semen (71.46%) was lower than values for hens inseminated with watermelon inclusive extended semen (75.71%-80.39%). The optimal inclusion of 30%-40% watermelon in dextrose saline diluent enhance rooster semen kinetics, seminal oxidative stability and egg fertility.

Improvement of oral contraceptive-induced glucose dysregulation and dyslipidemia by valproic acid is independent of circulating corticosterone

Olatunji, Lawrence A.,Omolekulo, Tolulope E.,Usman, Taofeek O.,Kim, InKyeom Informa UK (Informa Healthcare) 2016 Archives of physiology and biochemistry Vol.122 No.3

Protective effect of soursop (Annona muricata linn.) juice on oxidative stress in heat stressed rabbits

( Olatunji Abubakar Jimoh ),( Eyanlola Soladoye Ayedun ),( Waheed Abimbola Oyelade ),( Olugbenga David Oloruntola ),( Olajumoke Temidayo Daramola ),( Simeon Olugbemiga Ayodele ),( Idowu Samuel Omoniyi 한국축산학회(구 한국동물자원과학회) 2018 한국축산학회지 Vol.60 No.11

Background: Preventing oxidative stress in heat stressed animals may be possible by increasing antioxidant defence via exogenous administration of antioxidant substrate and/or its precursors. The study aimed to investigate the effect of Soursop juice in mitigating oxidative stress induced by heat stress in rabbit. Methods: Sixty mixed breed rabbit bucks aged 12-18 months old with the average weight of 1826 ± 8.35 g/rabbit, randomly allotted to experimental treatments of four replicates each, in a completely randomized design during high-temperature humidity index in Ado Ekiti, Southwest Nigeria. Soursop juice (SSJ) was administered via oral drenched daily per kg body weight (BW), to designated treatment 1 to 5; 0.55 mlkg^-1BW distilled water (control), 0.55 mlkg^-1BW SSJ, 1.11 mlkg^-1BW SSJ, 1.67 mlkg^-1BW SSJ and 2.22 mlkg-1BW SSJ, respectively. Fastened blood samples were collected at days 28 and 56, and assay for serum protein, cholesterol, triglycerides, superoxide dismutase, catalase, reduced glutathione and lipid peroxidation using standard procedures. Result: Result revealed that SSJ demonstrated hypocholesterolemic effect in a dose-dependent manner throughout the study. Effect of chronic administration of SSJ to heat stressed rabbits proved beneficial, as SSJ reduced serum lipid peroxidation and enhanced antioxidant activity over 8 weeks. Conclusion: Administration of soursop juice to heat-stressed bucks at 2.22 mlkg^-1BW offered optimum antioxidant defense against oxidative stress.

Potentials of Phyllanthus amarus, Viscum album and Moringa oleifera supplements to mitigate heat stress in female rabbits in humid tropics

Olatunji Abubakar Jimoh,Blessing Oluwaseun Olakanye,Ayoola Mercy Ajewole,Saanu Olajumoke Akinbuyide,Johnson Sunday Adetifa,Abdul-Quadri Ayodeji Jimoh,Adewale Oluwatosin Mayowa,Feyisayo Pemisire Adesin 한국축산학회 2024 한국축산학회지 Vol.66 No.1

Global warming is a key challenge subjecting animals to heat stress conditions resulting in multiple physiological alterations in tropical climate. Dietary approach seems to be the more friendly approach to curb the adverse effects of heat stress in rabbits. Some herbs have been categorized to have high potential for promotion of immune responses for amelioration of heat stress. Thus, this research aims to evaluate the potential of Mistletoe (Viscum album), Moringa (Moringa oleifera) and Phyllanthus (Phyllanthus amarus) leaf meal as herbal supplements for the alleviation of heat stress in female rabbits by measuring improvement in sex and stress hormonal responses in serum biochemistry. 80 Rabbit does were exposed to 4 dietary groups supplemented with each of Mistletoe, Moringa, Phyllanthus and a control in an 84-day trial at the summit of thermal stress in South west Nigeria. Growth indices were monitored throughout the study, blood samples were compiled at the end of the trial to assess serum biochemistry, stress and sex hormonal responses of the Does using standard protocols. The results revealed that final weight and weight gain of Does fed on Phyllanthus were significantly (p < 0.05) higher (11.46% and 14.25%, respectively) than Does on control. The herbal supplements enhance glucose, protein, albumin and globulin, reduced cholesterol, and creatinine of Does under heat stress conditions. Among the herbal treatment groups, mistletoe, moringa and phyllanthus had 12.42%, 18.39% and 16.90%, respectively, lower corticosterone than control groups which had 39.76ng/ml. Triiodothyronine of Does fed control were significantly (p < 0.05) lower than Does on Moringa oleifera and Phyllanthus amarus supplements. Estradiol and Follicle stimulating hormone of rabbit Does fed on moringa supplement were significantly (p < 0.05) higher than other treatments. In conclusion, the herbal supplements tend to mitigate the detrimental outcome of thermal stress on Does by suppressing stress hormones. Moringa oleifera and Phyllanthus amarus enhanced sex hormones while Phyllanthus amarus confered growth promoting effects on the Does.

Milk yield and kit development of four breeds of rabbit in Ibadan, Nigeria

( Olatunji Abubakar Jimoh ),( Emmanuel Olabisi Ewuola ) 한국축산학회(구 한국동물자원과학회) 2017 한국축산학회지 Vol.59 No.12

Background: Rabbit breeding with high performance imported ones would be of benefit for genetic diversity and improvement of performance in domestic rabbit breeds. The rearing of more productive rabbit breeds could be pathway to improve the productivity and reduce the production cost. Maternal nutritional status exert a great influence on reproductive functions of does, which may expand from conception, through gestation and parturition and development of kits to puberty. Methods: Four breeds of rabbit were evaluated for their parturition, weaning and pubertal differences among the rabbit population in Ibadan, Nigeria. The breed consist of Fauve De Bourgogne (FDB), Chinchilla (CHA), British Spot (BS) and New Zealand White (NZW) rabbits. A total of 60 bucks and 360 does consisting of 15 bucks and 90 does per breed were mated in 6 mating cycles, three each of natural mating and artificial insemination. All does were synchronized for estrus with 20 IU pregnant mare serum gonadotropin 48 h prior to mating. The does after parturition were assessed for milk yield (g) and kit survival rate (%) till weaning, weight changes of kits from birth to puberty. At puberty, the pubertal age (days) and weight (g) of the offspring were assessed. Result: Results obtained reveals that British Spot doe had highest milk yield among the breeds which significantly increased growth of kit and weight at weaning in British Spot rabbits. Survival rates of Chinchilla kits were significantly (p < 0.05) higher than Fauve de Bourgogne, British Spot and New Zealand White kits. Puberty attainment of the rabbits indicates that British spot does and Fauve de Bourgogne bucks are early maturing. Conclusion: Chinchilla shows high kit survivability and British spot has highest milk yield among the four breeds of rabbit.

Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder

Olatunji, Lawrence A.,Usman, Taofeek O.,Seok, Young-Mi,Kim, In-Kyeom Informa UK (Informa Healthcare) 2017 Archives of physiology and biochemistry Vol.123 No.1

Combined oral contraceptive and nitric oxide synthesis inhibition synergistically causes cardiac hypertrophy and exacerbates insulin resistance in female rats

Olatunji, Lawrence A.,Olaniyi, Kehinde S.,Usman, Taofeek O.,Abolarinwa, Bilikis A.,Achile, Caleb J.,Kim, In-kyeom Elsevier 2017 Environmental toxicology and pharmacology Vol.52 No.-

<P><B>Abstract</B></P> <P>Combined oral contraceptive (COC) use or inhibition of nitric oxide (NO) synthesis has been shown to cause hypertension and insulin resistance. However, the concomitant effects of COC and NO deficiency on the heart and glucose regulation are not well known. We therefore hypothesized that COC treatment during NO deficiency would lead to the development of cardiac hypertrophy that is associated with aggravated glucose deregulation, pro-inflammatory and pro-fibrotic biomarkers. Eight-week-old female Wistar rats were randomly allotted into control, NO deficient (<I>N</I> <SUP>G</SUP>-nitro-<SMALL>L</SMALL>-arginine methyl ester: L-NAME; 20.0mg/kg <I>b.w.</I>), COC-treated (1.0μg ethinylestradiol+5.0μg levonorgestrel, p.o) and L-NAME+COC-treated groups. The animals were treated daily for 6 weeks. Systolic blood pressure was estimated by tail-cuff plethysmography, insulin resistance (IR) and β-cell function were estimated by homeostatic model of assessment (HOMA-IR and HOMA-β). Pro-inflammatory (C-reactive protein; CRP and uric acid) and pro-fibrotic (plasminogen activator inhibitor-1; PAI-1) biomarkers were estimated in the plasma. Cardiac histological examination was also done. Results show that COC or L-NAME treatments led to increased blood pressure, HOMA-IR, impaired β-cell function, PAI-1, CRP and uric acid, without significant effect on cardiac mass. L-NAME+COC-treated group had significantly higher blood pressure, HOMA-IR, impaired β-cell function, PAI-1, CRP and cardiac mass than COC- or L-NAME-treated groups. Histological examination validated that COC use during NO deficiency causes cardiac hypertrophy. The present study demonstrates that COC treatment and NO deficiency synergistically causes cardiac hypertrophy that is associated with aggravated glucose deregulation, atherogenic dyslipidemia, pro-inflammatory and pro-fibrotic markers.</P> <P><B>Highlights</B></P> <P> <UL> <LI> COC treatment or NO deficiency induces high blood pressure and cardiometabolic disturbances. </LI> <LI> COC treatment or NO deficiency does not result in increased cardiac mass. </LI> <LI> COC treatment and NO deficiency synergistically causes cardiac hypertrophy. </LI> <LI> COC treatment during NO deficiency exacerbates cardiometabolic disturbances. </LI> </UL> </P>

A Teleoperated Robotic Catheter System with Motion and Force Feedback for Vascular Surgery

Olatunji Mumini OMISORE,ShiPeng HAN,LingXue REN,Lei WANG 제어로봇시스템학회 2018 제어로봇시스템학회 국제학술대회 논문집 Vol.2018 No.10

In recent time, robotic catheter systems have been proposed to minimize major limitations in minimally invasive vascular surgery. Technical evaluations of conventional systems have shown that teleoperation of robot-based systems could provide feasible and safe treatment method for vascular abnormalities. However, despite the merits, robot-assisted vascular surgeries are performed in fewer clinical centres, worldwide. In this study, a 2-DoF teleoperated robotic catheter system is proposed for treatment of vascular catheterization. The system provides visual feedbacks of motion and force information during catheterization procedures. Operation of the robotic catheter system was experimentally validated using master-slave teleoperation system. In the experiments, different phantom models of human vasculature were catheterized and the results obtained shows that the proposed robotic catheter system can provide useful motion and force feedback for vascular surgery.