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      • Karyotype and RFLP of the nuclear rDNA of Crocidura sp. on Cheju Island, South Korea (Mammalia, Insectivora)

        IWASA, M.A.,OHDACHI, S.,HAN, S.-H.,OH, H.-S.,ABE, H.,SUZUKI, H. 濟州大學校 基礎科學硏究所 2003 基礎科學硏究 Vol.16 No.2

        We revised the taxonomic status of a white-toothed shrew (Insectivora, Mammalia) on Cheju Island, South Korea, which has been regarded as japanese white-toothed shrew, Crodidura dsinezumi, by analyzing karyotype and restriction fragment length polymorphism (RFLP) for the nuclear ribosoma RNA gene(rDNA) spacer regions. Karyotype of a specimen of Crocidura from Cheju Island was the same as in lesser white-toothed shrew, C. suaveolens, from Tsushima Island and the Eurasian Continent, carrying 2n=40 and FN (fundamental arm number of diploid autosomal set) = 46, whereas C. dsinezumi from the Japanese Islands shows 2n=40 and FN=52. The rDNA-RFLP was examined in two specimens of Crocidura from Cheju Island, two C. dsinezumi from Japan (Honshu and Kyusuh), and one C. suaveolens from the Korean Peninsula, using six restriction sites with the 28S mouse rDNA probe. The shrews from Cheju Islnad possessed four restriction sites common to C. suaveolens from the Koeran Peninsula but only one site common to C. dsinezumi, indicating the shrews from Cheju Island are genetically more close but distinct from the Korean Penisula populations of C. suaveolens. From the results of karyotype and rDNA-RFLP, the specimens from Cheju Island can be regarded as C. suaveolens as a taxonomic unit. Nous avons re´vise´ le statut taxonomique d'une musaraigne a` dents blanched (Mammalia, Insectivora) de l'ile cheju, Core´e du Sud, qui a e´te´ conside´re´e comme la musaraigne japonaise a` dents blanches Crocidura dsinezumi d'apre's I'analyse de son caryotype et de restructuration du polymorphisme d'un fragment de longueur(RFLF) pour les re´gions plus espace´es du ge`ne ribosomal nucle´aire RNA (rDNA). Le caryotype d'un spe´cimen de Crocidura de l'ile Cheju e´tait le me^me qui celui de la petite Crocldura, C. suaveolens, de l'ile Tsushima et du continent eurasien qui a 2n=40 et NF=46, tandis que C. dsinezumi des i^les japonaises a 2n=40 te NF=52. Le rDNA-RFLP a e´te´ examine´ dans deux spe´cimens de Crocidura de l'ile Cheju, deux C. dsinezumi du Japon (Honshu et Kyushu), et un C. suavelens de la pe´ninsule de Core´e, utilisant six sites de restriction avec la sonde rDNA 285 de la souris. Les musaraignes de l'ile Cheju pre´sentent quatre sites de restriction en commun avec C. suaveolens de la pe´ninsule de Core´e, mais seulement un site en commun avec C. dsinezumi, ce qui indique que les musaraignes de l'ile de Cheju sont ge´ne´tiquement plus proches mais distinctes des population de C. suaveolens de la pe´ninsule de core´e. D'apre`s re´sultats du caryotype de RFLP et de rDNA, les spe´cimens de l'ile de Cheju peuvent e^tre regarde´s comme des C. suaveolens.

      • Molecular phylogeny of Crocidura shrews in northeastern Asia : A special reference to specimens on Cheju Island, South Korea

        HAN, Sang-Hoon,IWASA, Masahiro A.,OHDACHI, Satoshi D.,OH, Hong-Shik,SUZUKI, Hitoshi,TSUCHIYA, Kimiyuki,ABE, Hisashi 濟州大學校 基礎科學硏究所 2003 基礎科學硏究 Vol.16 No.2

        Molecular phylogeny of crodicurine shrews(Insectivora, Soricidae) in northeastern Asia was investigated to confirm the taxonomic status of unidentiffied specimens of Crodicura from Cheju Island, South Korea. Phylogenetic trees were construced by neighbor-joining (NJ) and maximum likelihood(ML) methods, based on mitochondrial cytochrome b gene sequences (402 base pairs) of 37 individuals of seven crocidurine species and three unidentified specimens from 31 localities mainly in northeastern Asia. Phylogenetic position of the three unidentified specimens from Cheju Island were compared with those of Suncus nurinus, C. attenuata, C. dsinezumi, C. lasiura, C. sibirica, C. suaveolens, and C. watasei. Both in NJ and ML trees, the three unidentified specimens were included in the cluster of C. dsinezumi and were obviously different from C. suaveolens on Cheju Island. Thus, the present investigation demonstrated that both C. suaveolens and C. dsinezumi exist on Cheju Island. Asiatic Black bear Management, Team Southern branch office at Mt. Jiri, 511-1 Whangeon-ri, Masan-meon, Gurye-gun, Jeolla-Namdo 542-853, Republic of Korea, e-mail : kwirc@ehol, com(SHH); Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan, e-mail : iwasam@vetemed. hokudai, ac.jp (MAI, HS) ; Institute of Low Temperatures Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan, e-mail : ohd@pop.lowtem.hokudai.ac.jp (SDO); Cheju National University, Ara-dong 1, Cheju, Cheju-do 690-756, Republic of Korea (HSO) ; Tokyo University of Agriculture, Atsugi, Kanagawa 243-0034, Japan (KT) ; Katsuraoka 26-17, Otaru, Hokkaido 047-0264, Japan (HA)

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