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RISS 인기검색어
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- 저자 : Nuzhdin, Sergey V. (검색결과 1 건)
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Bergland, Alan O.,Chae, Hyo-seok,Kim, Young-Joon,Tatar, Marc,Nuzhdin, Sergey V. Public Library of Science 2012 PLoS genetics Vol.8 No.4
<▼1><P>To gain insight into the molecular genetic basis of standing variation in fitness related traits, we identify a novel factor that regulates the molecular and physiological basis of natural variation in female <I>Drosophila melanogaster</I> fecundity. Genetic variation in female fecundity in flies derived from a wild orchard population is heritable and largely independent of other measured life history traits. We map a portion of this variation to a single QTL and then use deficiency mapping to further refine this QTL to 5 candidate genes. Ubiquitous expression of RNAi against only one of these genes, an aquaporin encoded by <I>Drip</I>, reduces fecundity. Within our mapping population <I>Drip</I> mRNA level in the head, but not other tissues, is positively correlated with fecundity. We localize <I>Drip</I> expression to a small population of corazonin producing neurons located in the dorsolateral posterior compartments of the protocerebrum. Expression of <I>Drip</I>–RNAi using both the pan-neuronal <I>ELAV</I>-<I>Gal4</I> and the <I>Crz-Gal4</I> drivers reduces fecundity. Low-fecundity RILs have decreased <I>Crz</I> expression and increased expression of <I>pale</I>, the enzyme encoding the rate-limiting step in the production of dopamine, a modulator of insect life histories. Taken together these data suggest that natural variation in <I>Drip</I> expression in the corazonin producing neurons contributes to standing variation in fitness by altering the concentration of two neurohormones.</P></▼1><▼2><P><B>Author Summary</B></P><P>A major goal of modern evolutionary biology is to elucidate the genetic basis of standing genetic variation underlying fitness traits. This goal is important for a comprehensive picture of the evolutionary process, because it allows us to understand the mode of natural selection on fitness traits and identify the molecular and physiological processes that affect fitness traits. Here, we describe our work to identify the molecular genetic and physiological basis for natural variation in a core life history trait, fecundity, of <I>Drosophila melanogaster</I>. Using a variety of mapping techniques, we show that differential expression of the aquaporin <I>Drip</I> in nervous tissue affects natural variation in female fecundity. We further go on to describe a novel domain of expression of <I>Drip</I> in neurons that produce the insect stress hormone corazonin and demonstrate that differential expression of <I>Drip</I> in these neurons affects female fecundity putatively through modulating the concentration of corazonin and dopamine. This surprising and novel observation highlights the benefit of exploiting natural genetic variation to identify the molecular processes underlying phenotypic traits.</P></▼2>
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