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        Microbial Community Analysis of a Coastal Hot Spring in Kagoshima, Japan, Using Molecular- and Culture-based Approaches

        Minako Nishiyama,Shuichi Yamamoto,Norio Kurosawa 한국미생물학회 2013 The journal of microbiology Vol.51 No.4

        Ibusuki hot spring is located on the coastline of Kagoshima Bay, Japan. The hot spring water is characterized by high salinity, high temperature, and neutral pH. The hot spring is covered by the sea during high tide, which leads to severe fluctuations in several environmental variables. A combination of molecular- and culture-based techniques was used to determine the bacterial and archaeal diversity of the hot spring. A total of 48 thermophilic bacterial strains were isolated from two sites (Site 1: 55.6°C; Site 2: 83.1°C) and they were categorized into six groups based on their 16S rRNA gene sequence similarity. Two groups (including 32 isolates)demonstrated low sequence similarity with published species,suggesting that they might represent novel taxa. The 148clones from the Site 1 bacterial library included 76 operational taxonomy units (OTUs; 97% threshold), while 132clones from the Site 2 bacterial library included 31 OTUs. Proteobacteria, Bacteroidetes, and Firmicutes were frequently detected in both clone libraries. The clones were related to thermophilic, mesophilic and psychrophilic bacteria. Approximately half of the sequences in bacterial clone libraries shared <92% sequence similarity with their closest sequences in a public database, suggesting that the Ibusuki hot spring may harbor a unique and novel bacterial community. By contrast, 77 clones from the Site 2 archaeal library contained only three OTUs, most of which were affiliated with Thaumarchaeota.

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        The Japan Health Physics Society Guideline on Dose Monitoring for the Lens of the Eye

        Yokoyama Sumi,Tsujimura Norio,Hashimoto Makoto,Yoshitomi Hiroshi,Kato Masahiro,Kurosawa Tadahiro,Tatsuzaki Hideo,Sekiguchi Hiroshi,Koguchi Yasuhiro,Ono Koji,Akiyoshi Masahumi,Kunugita Naoki,Natsuhori 대한방사선방어학회 2022 방사선방어학회지 Vol.47 No.1

        Background: In Japan, new regulations that revise the dose limit for the lens of the eye (hereafter the lens), operational quantities, and measurement positions for the lens dose were enforced in April 2021. Based on the international safety standards, national guidelines, the results of the Radiation Safety Research Promotion Fund of the Nuclear Regulation Authority, and other studies, the Working Group of Radiation Protection Standardization Committee, the Japan Health Physics Society (JHPS) developed a guideline for radiation dose monitoring for the lens.Materials and Methods: The Working Group of the JHPS discussed the criteria of non-uniform exposure and the management criteria set not to exceed the dose limit for the lens.Results and Discussion: In July 2020, the JHPS guideline was published. The guideline consists of three parts: main text, explanations, and 26 examples. In the questions, the corresponding answers were prepared, and specific examples were provided to enable similar cases to be addressed.Conclusion: With the development of the guideline on radiation dose monitoring of the lens, radiation managers and workers will be able to smoothly comply with revised regulations and optimize radiation protection.

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