http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Expression of an M Cell-specific Ligand-fused FMDV Epitope Using Saccharomyces cerevisiae
Ngoc LE MY TIEU,Hee-Won SEO,Kum-Kang SO,Jeesun CHUN,Dae-Hyuk KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
The mucosal surface forms a layer between our body and the out environment to protect from invasion of pathogens such an antigen, bacteria, and so on. Since that, mucosal vaccine was developed and has several advantages as an effective alternative to vaccines through a systemic route. M cells express a large amount of immune surveillance receptors on the apical surface. One of the M cell ligands, ligand A was proved that could promote the uptake of fusion antigen and enhance the immune response against the fused antigen, therefore ligand A can be used as an adjuvant for targeted delivery into the mucosal system. Our study focused on producing an oral vaccine against Foot and Mouth Disease (FMD) virus, using Saccharomyces cerevisiae as the expression host to express a yeast codon optimized (op) gene encoding for VP1 antigen (op1). We used M cell-targeting ligand A fused VP1 of FMDV in order to develop the oral mucosal vaccine against FMDV infection. M cell-targeting ligand A-conjugated VP1 interacted efficiently with M cells of Peyer’s patch. This construct gene was cloned into mucosal vaccine, M cell ligands, FMD virus, VP1 antigena yeast episomal vector and the recombinant plasmid was transformed into S. cerevisiae. Transformation was confirmed by colony PCR and E. coli back transformation. Expression was examined through Western blot. Western blot showed signal of the target proteins, indicated that the protein product of the cloned gene was successfully expressed.
Pham Duc Trung,팜황란,Le Hien T. Ngoc,Tieu My-Van,Kim Daesoo,Kim Jae Young,Cho Sungbo 한국바이오칩학회 2024 BioChip Journal Vol.18 No.1
Macrophages are immune cells that play important roles in the human body’s initial immune responses against pathogens and tumor cells. We investigated the use of electrical impedance monitoring to assess the differentiation of THP-1 mono- cyte into macrophages, which is necessary for immunotherapy research conducted. The change in resistance at 1 kHz and capacitance at 100 kHz measured were proportionally increased according to not only the increase in the density of resting macrophages differentiated by Phorbol-12-myristate-13-acetate treatment but also the initial number of THP-1 cells seeded on the electrode. Additionally, real-time impedance data from THP-1 cells after 48 h of cultivation demonstrated greater recognition of the resting macrophage phenotypes (adhesion cells) covered microelectrode surface with a significant increase of impedance signal in comparison with monocytes phenotypes (suspended cells). Furthermore, during the polarization phase of macrophages, the alternatively activated macrophage phenotype was larger and fl atter than that of classically activated macrophage and resting macrophage phenotypes, indicating a correlation with a higher resistance and lower capacitance impedances at 1 kHz and 100 kHz of alternatively activated macrophages (4750 Ω and – 3.5 nF) than that of classically activated macrophages (2000 Ω and – 1.5 nF) and resting macrophages (3500 Ω and – 2.0 nF), respectively. The study’s findings demonstrated that the impedance measurement system is high sensitivity and confi dence in monitoring macrophages differentiation and polarization. The electrical impedance, which has significance for each macrophage phenotype, is com- patible with macrophages characteristic features observed using flow cytometry and a microscope.