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Felhi, Samir,Baccouch, Noura,Salah, Hichem Ben,Smaoui, Slim,Allouche, Noureddine,Gharsallah, Neji,Kadri, Adel 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.6
The present study revealed that the nutritive value of grape seeds (Vitis vinifera L.) was $383.55{\pm}0.13Kcal/100g$, with magnesium as the most abundant mineral element ($70.44{\pm}0.88 mg/L$). The maximum phenolic ($392.58{\pm}1.70mg\;of\;GAE/g$), flavonoid ($256.16{\pm}1.60mg\;of\;QE/g$), and tannin ($30.95{\pm}0.17mg\;of\;CE/g$) contents were also found in the ethanol, dichloromethane, and hexane extracts, respectively. The major phytochemical compounds in the ethyl acetate extract were identified via gas chromatography-mass spectrometry (GC-MS) analysis. The ethanol extract has the highest antioxidant activity ($IC_{50}=140{\pm}1.20{\mu}g/mL$ for DPPH, $145.28{\pm}0.45mg$ ${\alpha}-tocopherol/g$ for total antioxidant capacity, and $EC_{50}=80{\pm}1.41{\mu}g/mL$ for ferric-reducing power assays). For ${\beta}-carotene$ test, the highest antioxidant activity was obtained in the hexane extract. A satisfactory antimicrobial activity was found against a panel of microorganisms with the ethyl acetate extract as the best antimicrobial agent. Additionally, it was found that the bactericidal concentration required for the grape seed extract to kill Listeria monocytogenes should be less than 12.50 mg/mL (minimum inhibitory concentration=4).
Sana Bakari,Marwa Ncir,Samir Felhi,Hafedh Hajlaoui,Mongi Saoudi,Neji Gharsallah,Adel Kadri 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.6
Total phenolic and flavonoid contents and antioxidant properties of extracts and essential oils of the aerial parts of Teucrium polium growing in Tunisia were evaluated. Total phenolic and flavonoid contents of extracts ranged from 48.88±0.01 to 400.00±0.01 mg of GAE/g and from 2.75±0.01 to 38.85±0.04mg of QE/g, respectively. GC/MS analysis of essential oils led to identification of 71 compounds representing 89.66% of the total composition. Antioxidant activities were assessed using the DPPH. (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity, β-carotene bleaching, and reducing power. The best overall antioxidant activity was recorded for acetone extracts, with a strong positive correlation between the total phenolic content and the IC50 value of DPPH (R²=0.9999), attributed to the polyphenolic content.