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RISS 인기검색어
- 검색키워드
- 저자 : Narpat S. Shekhawat (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
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High Frequency Plantlet Regeneration from Nodal Segment Culture of Female Momordica dioica (Roxb.)
Shekhawat, Mahipal S.,Shekhawat, Narpat S.,Harish, Harish,Ram, Kheta,Phulwaria, Mahendra,Gupta, Amit Kumar 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.2
An in vitro propagation method for female plants of Momordica dioica (Roxb.) has been established. The nodal segments were harvested and the cut ends of the explants were sealed with wax and then surface sterilized and cultured. Bud breaking occurred on Murashige and Skoog's (MS) agar-gelled medium + 2.0 mg $L^{-1}$ 6-Benzylaminopurine (BAP) + 0.1 mg $L^{-1}$ Indole-3 acetic acid (IAA). The cultures were amplified by passages on MS medium supplemented with 1.0 mg $L^{-1}$ BAP + 0.1 mg $L^{-1}$ IAA. Further, shoot amplification (29.2 shoots per vessel) was achieved by subculturing of in vitro regenerated shoot clump on MS medium + 0.5 mg $L^{-1}$ BAP + 0.1 mg $L^{-1}$ IAA. The micropropagated shoots were subsequently transferred for root formation on half-strength MS medium + 2.0 mg $L^{-1}$ Indole-3 butyric acid (IBA) with 89% success rate. The in vitro-regenerated shoots were also rooted ex vitro with 34% success. These plantlets were hardened in the greenhouse and transferred to the field. The established protocol is suitable for true to type cloning of mature female plant of M. dioica.
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High Frequency Plantlet Regeneration from Nodal Segment Culture of Female Momordica dioica (Roxb.)
Mahipal S. Shekhawat,Narpat S. Shekhawat,Harish,Kheta Ram,Mahendra Phulwaria,Amit Kumar Gupta 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.2
An in vitro propagation method for female plants of Momordica dioica (Roxb.) has been established. The nodal segments were harvested and the cut ends of the explants were sealed with wax and then surface sterilized and cultured. Bud breaking occurred on Murashige and Skoog's (MS) agar-gelled medium + 2.0 mg L^(-1) 6-Benzylaminopurine (BAP) + 0.1 mg L^(-1) Indole-3 acetic acid (IAA). The cultures were amplified by passages on MS medium supplemented with 1.0 mg L^(-1) BAP + 0.1 mg L^(-1) IAA. Further, shoot amplification (29.2 shoots per vessel) was achieved by subculturing of in vitro regenerated shoot clump on MS medium + 0.5 mg L^(-1) BAP + 0.1 mg L^(-1) IAA. The micropropagated shoots were subsequently transferred for root formation on half-strength MS medium + 2.0mg L^(-1) Indole-3 butyric acid (IBA) with 89% success rate. The in vitro-regenerated shoots were also rooted ex vitro with 34% success. These plantlets were hardened in the greenhouse and transferred to the field. The established protocol is suitable for true to type cloning of mature female plant of M. dioica.
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