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Jo, Namgyeong,Kim, Bongjun,Lee, Sun-Mi,Oh, Jeseung,Park, In Ho,Jin Lim, Kook,Shin, Jeon-Soo,Yoo, Kyung-Hwa Elsevier 2018 Biosensors & bioelectronics Vol.102 No.-
<P><B>Abstract</B></P> <P>To prevent spread of infection and antibiotic resistance, fast and accurate diagnosis of bacterial infection and subsequent administration of antimicrobial agents are important. However, conventional methods for bacterial detection and antibiotic susceptibility testing (AST) require more than two days, leading to delays that have contributed to an increase in antibiotic-resistant bacteria. Here, we report an aptamer-functionalized capacitance sensor array that can monitor bacterial growth and antibiotic susceptibility in real-time. While <I>E. coli</I> and <I>S. aureus</I> were cultured, the capacitance increased over time, and apparent bacterial growth curves were observed even when 10 CFU/mL bacteria was inoculated. Furthermore, because of the selectivity of aptamers, bacteria could be identified within 1h using the capacitance sensor array functionalized with aptamers. In addition to bacterial growth, antibiotic susceptibility could be monitored in real-time. When bacteria were treated with antibiotics above the minimum inhibitory concentration (MIC), the capacitance decreased because the bacterial growth was inhibited. These results demonstrate that the aptamer-functionalized capacitance sensor array might be applied for rapid ASTs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Aptamer-functionalized capacitance sensor is developed to monitor AST in real-time. </LI> <LI> Apparent bacterial growth curves are obtained within 6 h even for 10 CFU/ml. </LI> <LI> Aptamer-functionalized capacitance sensor could identify bacterial growth within 1 h. </LI> </UL> </P>