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NANA NGUEFANG LAURE,안주희 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.2
This study was designed to evaluate the possibilityof using phage-amplification assay for discriminatingbetween antibiotic-sensitive and antibiotic-resistant SalmonellaTyphimurium. The characteristics of Salmonellaphage PBST32 were determined by adsorption rate, onestepgrowth curve, and lytic activity. The ability of phagebasedmethod to detect S. Typhimurium ATCC 19585(STCIP) was determined in single culture and bacterialmixtures of S. Typhimurium ATCC 19585 (STWT), Klebsiellapneumoniae, and Staphylococcus aureus. Theadsorption rates of PBST32 were 95% and 93% againstSTWT and STCIP after 20 min, respectively. The PBST32showed latent period of 20 min and average burst size of90 against STWT and STCIP. The STCIP was selectivelydetected in mixtures of S. aureus, K. pneumoniae, andSTWT by phage amplification assay. These results provideuseful information for designing phage amplificationmethod that can differentially detect antibiotic-resistantpathogens.