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José Francisco Díaz Nájera,Jaime Sahagún Castellanos,Mateo Vargas Hernández,Sergio Ayvar Serna,Omar Guadalupe Alvarado Gómez,Clemente Villanueva Verduzco,Marcelo Acosta Ramos 한국식물병리학회 2018 Plant Pathology Journal Vol.34 No.3
Fruit rot is the principal phytopathological problem of pipiana pumpkin (Cucurbita argyrosperma Huber) in the state of Guerrero. The aims of this research were to 1) identify the causal agent of southern blight on pumpkin fruits by morphological, pathogenic, and molecular analysis (ITS1, 5.8S, ITS2); 2) evaluate in vitro Trichoderma spp. strains and chemical fungicides; and 3) evaluate under rainfed field conditions, the strains that obtained the best results in vitro, combined with fungicides during two crop cycles. Number of commercial and non-commercial fruits at harvest, and seed yield (kg ha-1) were registered. Morphological, pathogenic and molecular characterization identified Sclerotium rolfsii as the causal agent of rot in pipiana pumpkin fruits. Now, in vitro conditions, the highest inhibition of S. rolfsii were obtained by Trichoderma virens strain G-41 (70.72%), T. asperellum strain CSAEGro-1 (69%), and the fungicides metalaxyl (100%), pyraclostrobin (100%), quintozene (100%), cyprodinil + fludioxonil (100%), and prochloraz (100%). Thiophanate-methyl only delayed growth (4.17%). In field conditions, during the spring-summer 2015 cycle, T. asperellum strain CSAEGro-1 + metalaxyl, and T. asperellum + cyprodinil + fludioxonil, favored the highest number of fruits and seed yield in the crop.
Valadez-Moctezuma Ernestina,Samah Samir,Mascorro-Gallardo J. Oscar,Reynoso José J. López,Marbán-Mendoza Nahum,Aranda-Osorio Gilberto,Brito-Nájera Guadalupe 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.6
Although Agave angustifolia Haw is one of the most cultural and industrial promising species in Mexico, no extensive genomic resources were available. Herein, we explore the transcriptome data set of this species. Total RNA from stem, roots, young and mature leaves was purified and sequenced by NovaSeq6000 system. From the de novo assembly of A. angustifolia, a total of 358,170 transcripts with average length of 1117 bp, N50 of 858 bp, and GC mean content of 43.5% were obtained. About 48.7% of the transcripts had open reading frame (ORF). Hence, 63.4% and 42.7% of the ORFs were annotated using Blastp and Blastx against UniProtKB database, respectively, uncovering the presence of at least 29,273 genes in the A. angustifolia transcriptome. The six pairwise comparisons between plant tissues resulted in the identification of 123,181 differentially expressed transcripts (23,978 were upregulated and 79,203 were downregulated). Several carbohydrate metabolism pathways were significantly enriched from the comparisons between leaves and stems. Thus, genes regulated for the fructan, starch and sucrose biosynthesis were discussed. Furthermore, genes involved in cellulose synthesis were explored being the members of the cellulose synthase genes subfamily were highly expressed in young leaf, while the members of the cellulose synthase-like subfamily were preferentially expressed in mature leaf. Moreover, A. angustifolia transcriptome served for the identification of 30,766 SSR and 21,686 VNTR potential markers. Overall, the transcriptomic data generated in this study provide an invaluable resource to understand the biology of A. angustifolia, offering new insight to understand their physiology and adaptation.
A novel six-degrees-of-freedom series-parallel manipulator
J. Gallardo-Alvarado,R. Rodríguez-Castro,C. R. Aguilar-Nájera,L. Pérez-González 대한기계학회 2012 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.26 No.6
This paper addresses the description and kinematic analyses of a new non-redundant series-parallel manipulator. The primary feature of the robot is to have a decoupled topology consisting of a lower parallel manipulator, for controlling the orientation of the coupler platform,assembled in series connection with a upper parallel manipulator, for controlling the position of the output platform, capable to provide arbitrary poses to the output platform with respect to the fixed platform. The forward displacement analysis is carried-out in semi-closed form solutions by resorting to simple closure equations. On the other hand; the velocity, acceleration and singularity analyses of the manipulator are approached by means of the theory of screws. Simple and compact expressions are derived here for solving the infinitesimal kinematics by taking advantage of the concept of reciprocal screws. Furthermore, the analysis of the Jacobians of the robot shows that the lower parallel manipulator is practically free of singularities. In order to illustrate the performance of the manipulator, a numerical example which consists of solving the inverse/forward kinematics of the series-parallel manipulator as well as its singular configurations is provided.
Ivonne Domínguez-Martínez,Ofelia Gabriela Meza-Márquez,Guillermo Osorio-Revilla,José Proal-Nájera,Tzayhrí Gallardo-Velázquez 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.1
Fourier transform mid-infrared (Mid-FTIR) spectroscopyin conjunction with multivariate analysis was used to predict thecapsaicin content, ascorbic acid, total phenolic compounds, andantioxidant activity of Capsicum annuum L. variety serrano. Twomultivariate calibrations, partial least square (PLS), and principalcomponent regression (PCR) were optimized to construct thecalibration models. The best models used to quantify the abovementioned compounds were obtained with the PLS algorithm andcoefficients of determination (R2) greater than 0.998 as well as astandard error calibration less than 0.098. The results demonstratedthat Mid-FTIR spectroscopy in combination with multivariateanalysis can be effectively used for to quantify the capsaicin,ascorbic acid, total phenol content, and antioxidant activity ofCapsicum annuum var. serrano. Mid-FTIR spectroscopy incombination with multivariate calibration offers rapid, easy samplepreparation, is environmentally friendly, and is operationallyuncomplicated, demonstrating the significant advantages of thechemometric models compared with conventional methods ofanalysis.
Laura Cristina Moreno-Na´jera,Juan Arturo Ragazzo-Sa´nchez,Cristina Regla Gasto´n-Pen˜a,Montserrat Caldero´n-Santoyo 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.12
The application of emerging technologies suchas ultrasound, microwaves and high hydrostatic pressure,allows the extraction of compounds in a sustainable mannerfrom a vegetable matrix with a high value such asjackfruit leaf proteins (Artocarpus heterophyllus Lam). Currently, the main method of protein extraction is basedon the precipitation with the use of an aggressive solvent,therefore it is necessary to optimize extraction methodswith a minimum waste production. In the protein extractionof jackfruit leaves, we obtained a content of 84.1 mg/gusing solvents. On the other hand, emergent extractionssuch as ultrasound, microwaves, and high hydrostaticpressure showed concentrations of 96.3, 95.6 and147.3 mg/g, respectively. In addition, we found that thebest extraction agent was 0.5 M NaCl, offering a range ofpossibilities that support green technologies as an imperativechange in the food industry.