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RISS 인기검색어
- 검색키워드
- 저자 : Muhammad Nurul Amin (검색결과 1 건)
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Md. Mahabubur Rahman,Muhammad Nurul Amin,Futoshi Ishiguri,Shinso Yokota,Rubaiyat Sharmin Sultana,Yuya Takashima,Kazuya Iizuka,Nobuo Yoshizawa 한국식물생명공학회 2009 Plant biotechnology reports Vol.3 No.3
A plantlet regeneration protocol was developed on pot-grown mature plants of Elaeocarpus robustus Roxb. cv. Dwarf from nodal and leaf explants. The best yield of adventitious shoots was achieved from the leaf-derived calli in a modified MS (MMS1, half strength of major salts, full strength of minor salts, and vitamins) medium containing 4.0 lM BA ? 4.0 lM Kn ? 0.5 lM NAA ? 15% coconut water (CW). The shoot multiplication rate was amplified about twofold per culture after the addition of 15% CW to the medium. The rate of shoot multiplication reached maximum at the 5th subculture, and it maintained this rate throughout the 3 subsequent subcultures. The best rooting in vitro was investigated by subculturing the microcuttings in an MMS2 (half strength of both major salts and minor salts and full strength of vitamins) medium containing 1.0 lM IBA in the dark for one initial week at 30C, followed by subculturing them in a plant-growth regulator (PGR)-free medium in the light. The plantlets raised in vitro were successfully established under ex vitro conditions. A plantlet regeneration protocol was developed on pot-grown mature plants of Elaeocarpus robustus Roxb. cv. Dwarf from nodal and leaf explants. The best yield of adventitious shoots was achieved from the leaf-derived calli in a modified MS (MMS1, half strength of major salts, full strength of minor salts, and vitamins) medium containing 4.0 lM BA ? 4.0 lM Kn ? 0.5 lM NAA ? 15% coconut water (CW). The shoot multiplication rate was amplified about twofold per culture after the addition of 15% CW to the medium. The rate of shoot multiplication reached maximum at the 5th subculture, and it maintained this rate throughout the 3 subsequent subcultures. The best rooting in vitro was investigated by subculturing the microcuttings in an MMS2 (half strength of both major salts and minor salts and full strength of vitamins) medium containing 1.0 lM IBA in the dark for one initial week at 30C, followed by subculturing them in a plant-growth regulator (PGR)-free medium in the light. The plantlets raised in vitro were successfully established under ex vitro conditions.
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