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      • What to expect: Classical and ambient collisions within this binary universe with "softer shadows" (original music composition)

        Molk, David Christopher Princeton University 2016 해외박사(DDOD)

        RANK : 247343

        소속기관이 구독 중이 아닌 경우 오후 4시부터 익일 오전 9시까지 원문보기가 가능합니다.

        BT's 2006 album, This Binary Universe, represents a major departure in compositional aims for someone whose career up to that point was rooted in EDM (electronic dance music). Here, BT states that he purposefully incorporates classical form within the album. This dissertation investigates the ways in which we might employ a classically oriented listening modality to address functional ambiguities that arise within the respective introductions. These ambiguities manifest from the inclusion of both classical and ambient-oriented processes and the tensions therein. An investigation into listener expectation provides a way to interact with the music, the processes that drive it, and our perception of these processes as we listen to the album. Chapter I examines how the overall cohesion within This Binary Universe enables comparisons not only from point to point within tracks but also across tracks. Chapter II scrutinizes the traditional role of introductions within the classical style, introducing the language of formal function and "becoming." Chapter III offers detailed analyses of the formal function ambiguity within the introductions of "All That Makes Us Human Continues," "The Internal Locus," "See You On The Other Side," and "The Antikythera Mechanism," discussing how the role of expectation within the listening process both engenders and responds to these functional frictions. Chapter IV reframes the specific findings of the four analyses within the context of the album as a whole, concluding with a survey of how the worlds of This Binary Universe continue to resonate in more recent projects by BT. The composition component that completes this dissertation, "softer shadows," incorporates a series of extended techniques that I've harvested slowly throughout my time in Princeton. murmur uses cardboard dowels standing in for more traditional mallets, creating a blurring of pitch and noise and culminating in a ping pong ball chorale. The second movement, fade to light, goes deeper still into these delicate worlds. These two movements allow us access to a softer shadow world. The motivation behind many of the techniques explored in "softer shadows" is my attempt to create novel acoustic analogues for electronic production techniques found within the EDM vocabulary.

      • Microtubule plus end binding proteins and nuclear movements in the Saccharomyces cerevisiae life cycle

        Molk, Jeffrey Nathan The University of North Carolina at Chapel Hill 2006 해외박사(DDOD)

        RANK : 247343

        소속기관이 구독 중이 아닌 경우 오후 4시부터 익일 오전 9시까지 원문보기가 가능합니다.

        Microtubules (MTs) are required for nuclear movements and chromosome segregation. MTs are polymers of tubulin protein with a defined polarity---the minus end is associated with the microtubule organizing center (MTOC) whereas the plus end probes the cellular environment. Additional proteins associated with the plus end act as adaptors between the MT and binding sites that facilitate nuclear and chromosome movements. The budding yeast Saccharomyces cerevisiae was used as a model system to examine two questions: (1) During mitotic spindle elongation, how does the cell ensure each daughter inherits a single MTOC? To investigate this, Mitotic Exit Network (MEN) proteins were analyzed in living cells. I find that, after anaphase onset, the MEN activator Tem1p accumulates persistently on the MTOC that enters the daughter cell. In contrast, the abundance of the MEN inhibitor Bub2p decreases during anaphase. This suggests that after anaphase onset, the presence of one MTOC in the daughter cell persistently signals mitotic exit. (2) By what mechanism do plus end binding proteins link oppositely oriented MT arrays? In the budding yeast mating pathway, the translocation of haploid nuclei toward each other is known as nuclear congression. I find nuclear congression is driven by MT plus end interactions. The plus end binding proteins Kar3p and Bik1p are required for efficient MT-MT interactions. Additionally, I find that during mitosis in vegetative cells, Kar3p and Bik1p are part of a family of plus end binding proteins that are required for anti-parallel MT interactions. Taken together, these data suggest a specific subset of plus end binding proteins function similarly during mating and mitosis on oppositely oriented MT arrays.

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