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RISS 인기검색어
- 검색키워드
- 저자 : Mohamed A. El-Tayeb (검색결과 1 건)
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Abdelnasser S. S. Ibrahim,Ali A. Al-Salamah,Ahmed Mohamed El-Toni,Mohamed A. El-Tayeb,Yahya B. Elbadawi,Garabed Antranikian 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.6
Recently isolated Cr(VI)-reducing AmphibacillusKSUCr3 whole cells were immobilised in magnetic gels. Magnetic magnetite (Fe3O4) nanoparticles were synthesisedwith an average particle size of 47 nm and 80 electromagneticunit (emu)/g saturation magnetisation. Whole cells wereimmobilised by entrapment in agar, agarose, alginate, orgelatin in the presence or absence of Fe3O4 nanoparticlesfor the preparation of both magnetic and nonmagneticimmobilised cells. Of the gels tested, alginate was selectedas the best immobilisation matrix, and following optimisationof the entrapment process, the immobilisation yield reached92.5%. In addition to the ease of separation and reuse ofthe magnetic cell-containing alginate beads using an externalmagnet, the magnetically immobilised cells showed approximately16% higher Cr(VI) reduction activity compared withnonmagnetic immobilised cells. To improve their physicaland mechanical properties, the magnetic alginate beadswere successfully coated with a dense silica layer usingsol–gel chemistry and Ca(OH)2, an alkaline catalyst fortetraethyl orthosilicate, to avoid leaching of Ca2+ ions. Amphibacillus KSUCr3 cells immobilised in silica-coatedmagnetic alginate beads showed approximately 1.4- to 3.9-fold enhancement of thermal stability compared with freecells. Furthermore, after seven batch cycles, the Cr(VI)reduction activity of free cells decreased to 48%, whereasimmobilised cells still retained 81.1% of their originalactivity. In addition, the Cr(VI)-reduction rate of immobilisedcells was higher relative to free cells, especially at higherCr(VI) concentrations. These results supported the developmentof a novel, efficient biocatalysts for Cr(VI) detoxificationusing a combination of whole cell immobilisation, sol-gelchemistry, and nanotechnology.
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