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Fractionation and Lipase-catalyzed Conversion of Microalgal Lipids to Biodiesel
김세원,Mizhang Xiao,신현재 한국생물공학회 2016 Biotechnology and Bioprocess Engineering Vol.21 No.6
This study was conducted to evaluate the lipid fractionation and purification procedures of lipase-catalyzed conversion of neutral lipids to microalgal biodiesel. Microalgae lipids were efficiently recovered and purified by a combined extraction method and crude lipid extracts were separated into neutral lipids, glycolipids, and phospholipids by solid-phase extraction. The high purity of the neutral lipids fraction was confirmed by its low concentration of phosphorous (< 2.0 ppm). Transesterification was catalyzed by immobilized Candida antarctica lipase for 72 h with stepwise addition of methanol. The reaction displayed Michaelis–Menten kinetics and produced high yields of microalgal biodiesel (91.2% in the case of Dunaliella salina) with a high content of unsaturated fatty acids (81.5%). Neutral lipids were converted to biodiesel by three-step transesterification, while the removal of polar lipids maintained the activity of the immobilized lipase by reducing both reaction mixture viscosity and contamination risk.
Advances in cultivation and processing techniques for microalgal biodiesel: A review
신현재,Mizhang Xiao,Qiuhua Dong 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.12
The key technologies for producing microalgal biodiesel include microalgae screening, economical cultivation,and efficient methods in lipid extraction and conversion. Recent advances in microalgae cultivation, lipid extraction,and biodiesel preparation are reviewed in this work, with emphasis on photosynthetic metabolisms, separation efficiency and catalytic kinetics. The mutual exclusion between lipid accumulation and fast growth limits total lipid productivity,while only triglycerides in neutral lipids are converted to biodiesel through transesterification. The hurdles in large scale culture and low neural lipids yield are discussed, as well as the relationship between high unsaturation and fuel properties. This review aims to provide technical information to guide strain screening and lipid conversion for microalgal biodiesel industry.