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Free Radical Scavenging Activity of Elsholtzia densa
Misba Khan,Rabia Hamid,Showkat Ahmad Ganie,Ishfak Hussain Wani,Bashir Ahmad Ganai,Akbar Masood,Mohmmad Afzal Zargar,Akhter Hussain Malik 사단법인약침학회 2012 Journal of Acupuncture & Meridian Studies Vol.5 No.3
Medicinal plants have been used traditionally to cure variety of diseases from times immemorial. Elsholtzia densa, a rare annual herb of Kashmir valley was assessed for its antioxidant efficacy. Antioxidant activity of the crude extracts was evaluated using DPPH, DNA sugar damage, lipid peroxidation, FTC and hydrogen peroxide scavenging assays. The percentage decrease of 1, 1-diphenyl-2-picryl hydrazyl radical (DPPH) standard solution was recorded maximum for the 50% ethanolic extract (90.48%). The extracts were further evaluated using the thiobarbituric acid-reactive species (TBARS) assay. The methanolic extract showed the highest activity (32.02%) in reducing oxidative damage to DNA. The antioxidant activity of the extracts were also determined using linoleic acid system and the highest antioxidant activity of 49.64% was found in 50% ethanolic extract. In case of FTC assay, the 50% ethanolic extract showed the highest activity (70.14%) which was comparable to that of α-tocopherol. Moreover, total phenolics concentration was found to be 62.5mg% and 77.5mg% in case of absolute ethanolic and 50% ethanolic extracts, respectively. These findings indicate promising antioxidant activity of crude extracts of the plant and need further exploration for its effective use in both modern and traditional system of medicines.
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