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        Effect of alfalfa habitat change on dispersal behavior of Harmonia axyridis Pallas and Hippodamia variegata Goeze (Coleoptera: Coccinellidae)

        Liu JunHe,Zhao Zhong,Yan Yan,Ali Abid,Ahmed Zeeshan,He DaHan,Yu Mingfu,Hang Jia,Perven Misbah,Nazir Tamsila,Tayyib Muhammad,Zhao ZiHua 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.4

        An alfalfa grassland landscape was divided into 36 plots during 2014 season in Yuanzhou District of Guyuan city in Ningxia Hui Autonomous Region of P.R. China. In these three times repeated experiments, treatment of habitat fragmentation was divided into two grades: continuity (H = 0.0) and fragmentation (H = 1.0), and their habitat loss rate was divided into 6 grades: 90, 80, 70, 50, 20 and 0%. Considering habitat loss 0% of the plot as the reference, as a result of sweeping net and visual methods, results showed that the flight distance of the Harmonia axyridis was much higher than that of the Hippodamia variegata both in fragmentation and continuous habitats. In the alfalfa landscape, H. axyridis moved more by flying, while H. variegata moved more by crawling. There was a threshold effect that affected the movement behavior of lady beetles between 70 and 100% of the habitat loss. The population of the two species of lady beetles in the continuous habitat was higher than that in the habitat fragmentation and was more obvious in 70 and 50% plots of the habitat loss. The dispersal rate of the two species of lady beetles inside plots was higher than outside of the experimental plots, which reflected the low diffusivity of the ladybeetle. Habitat fragmentation affected the dispersal, migration and construction of two species of lady beetles on different spatial scales by disturbing the spread of natural enemies and searching for prey.

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        Phylogeny and Evolution of Calcineurin B‑Like (CBL) Gene Family in Grass and Functional Analyses of Rice CBLs

        Min Jiang,Changling Zhao,Mingfu Zhao,Yongzhong Li,Guosong Wen 한국식물학회 2020 Journal of Plant Biology Vol.63 No.2

        Calcium signals play critical functions in regulating diverse arrays of plant growth and development and mediating a variety of biotic and abiotic stress responses as a second messenger. Calcineurin B-like (CBL) proteins were involved with plantspecifc Ca2+signaling as calcium sensors. In this work, we retrieved 152 CBL gene members from 15 diferent grass species, surveyed their phylogenetic relationships and sequence features and also performed expression patterns and functional analyses of rice CBLs. Phylogenetic analysis indicated that grass CBLs fall into four diferent groups (Group A–D). Sequence analysis showed that CBL proteins harboring four conserved calcium-binding EF-hand have key amino acid residues Asp and Glu which had relatively high proportion in the average abundance. Molecular evolutionary analyses revealed that group A, B and C CBLs in their evolution process sufered the purifying selection, while group D CBLs were subjected to positive selection. Moreover, expression analyses showed signifcant divergent expression patterns of OsCBLs in various organs and under diferent hormones and abiotic stresses. Furthermore, tolerance analysis revealed that OsCBL3 and OsCBL8 overexpression transgenic rice seedlings improved salt tolerance and OsCBL5, OsCBL6 and OsCBL7 positively regulated drought stress. In general, the domain and base sequence of the CBL gene family is highly conserved in grasses. OsCBL genes had specifc gene expression profles and function in diferent stresses.

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