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RISS 인기검색어
- 검색키워드
- 저자 : Michael D. A. Thomas (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
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Thermal evaluation of laser exposures in an<i>in vitro</i>retinal model by microthermal sensing
Choi, Tae Y.,Denton, Michael L.,Noojin, Gary D.,Estlack, Larry E.,Shrestha, Ramesh,Rockwell, Benjamin A.,Thomas, Robert,Kim, Dongsik SPIE - International Society for Optical Engineeri 2014 JOURNAL OF BIOMEDICAL OPTICS Vol.19 No.9
<P>A temperature detection system using a micropipette thermocouple sensor was developed for use within mammalian cells during laser exposure with an 8.6-μm beam at 532 nm. We have demonstrated the capability of measuring temperatures at a single-cell level in the microscale range by inserting micropipettebased thermal sensors of size ranging from 2 to 4 μm into the membrane of a live retinal pigment epithelium (RPE) cell subjected to a laser beam. We setup the treatment groups of 532-nm laser-irradiated single RPE cell and in situ temperature recordings were made over time. Thermal profiles are given for representative cells experiencing damage resulting from exposures of 0.2 to 2 s. The measured maximum temperature rise for each cell ranges from 39 to 73C; the RPE cells showed a signature of death for all the cases reported herein. In order to check the cell viability, real-time fluorescence microscopy was used to identify the transition of pigmented RPE cells between viable and damaged states due to laser exposure.</P>
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Concrete bridge deck deterioration model using belief networks
Hrodny Njardardottir,Brenda McCabe,Michael D. A. Thomas 한국계산역학회 2005 Computers and Concrete, An International Journal Vol.2 No.6
When deterioration of concrete is observed in a structure, it is highly desirable to determine the cause of such deterioration. Only by understanding the cause can an appropriate repair strategy be implemented to address both the cause and the symptom. In colder climates, bridge deck deterioration is often caused by chlorides from de-icing salts, which penetrate the concrete and depassivate the embedded reinforcement, causing corrosion. Bridge decks can also suffer from other deterioration mechanisms, such as alkali-silica reaction, freeze-thaw, and shrinkage. There is a need for a comprehensive and integrative system to help with the inspection and evaluation of concrete bridge deck deterioration before decisions are made on the best way to repair it. The purpose of this research was to develop a model to help with the diagnosis of concrete bridge deck deterioration that integrates the symptoms observed during an inspection, various deterioration mechanisms, and the probability of their occurrence given the available data. The model displays the diagnosis result as the probability that one of four deterioration mechanisms, namely shrinkage, corrosion of reinforcement, freeze-thaw and alkali-silica reaction, is at fault. Sensitivity analysis was performed to determine which probabilities in the model require refinement. Two case studies are included in this investigation.
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Howard, Paul,Day, Kathleen H.,Kim, Kyoon E.,Richardson, Jeanne,Thomas, James,Abraham,Irene,Fleischmann, Robert D.,Gottesman, Michael M.,Maurer, Richard A. 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-
The mechansims responsible for decreased levels of cAMP-dependent protein kinase activity in a mutant Chinese hamster ovary cell line have been examined. The cAMP-resistant Chinese hamster ovary 10260 cell line was found to possess only 20% of the cAMP-dependent protein kinase activity found in wild-type cells. The pressence of decreased concentrations of the catalytic subunit in these cells was confirmed through binding studies using a radiolabeled, heat-stable inhibitor of the kinase. Cloned Chinese hamster ovary catalytic subunit cDNAs were isolated, characterized, and used as hybridization probes to examine the relative concentrations of catalytic subunit mRNAs in the wild-type and 10260 cell lines. A 40-50% decrease in the concentration of the mRNA for the Cα isozyme of the catalytic subunit was observed in 10260 cells, as compared with wild-type. This decrease in catalytic subunit mRNA concentration probably accounts for a portion of the decreased kinase activity in the mutant cell. Further analysis of Cα mRNA by polymerase chain reaction confirmed the decreased expression of Cα mRNA in 10260 cells and further demonstrated the presence of two different species of Cα cDNAs was indistinguishable from the wild-type cDNA, but the other species was shorter. Nucleotide sequence analysis of the amplified cDNAs led to the identification of a 191-base pair deletion in the shorter cDNA. Gene transfer studies using wild-type and 10260 Cα cDNAs demonstrated wild-type activity, but the shorter cDNA was inactive. These studies suggest that at least two alternations in gene expression are responsible for decreased cAMP-dependent protein kinase activity in the 10260 cell line. One alteration results in an approximately 2-fold decrease in the concentrations of Cα mRNA in the cells.
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