Global warming mitigation by the forestry options in Bangladesh : a CDM perspective

Miae, Md. Danesh Kookmin Univ. 2006 국내박사

Effectiveness of Storytelling as a Teaching Strategy in English Education for Korean College Students

Janice Miae Lee Torch Trinity Graduate University 2016 국내석사

Korean education is generally known to be a typical rote learning style and English language education in South Korea has long been grammar-based. However, it has been often pointed out that these approaches have not been practical or effective. Therefore, it has been a great challenge for English educators in South Korea to find a more effective way to teach English. Since the practicality of English lessons has been greatly favored in most colleges, there has been a significant number of college-level compulsory courses being replaced by TOEIC-courses in many South Korean universities in order to successfully prepare the students for their future careers. This study, thus, examined the feasibility of integrating storytelling lessons into college English courses in order to provide an effective English teaching strategy free from the rote-learning style and improve students’ TOEIC scores at the same time. The purpose of this study was to explore the effectiveness of storytelling in English language education in South Korean colleges and the possibility of incorporating the outcomes of this study into college English classes for more practical English lessons that can be applied in everyday life. The primary research question of this study was: How can storytelling be used as an effective teaching strategy in helping Korean college students to improve their English communicative skills as compared to another typical teaching strategy that is currently used in college English classes? Subsidiary questions were: How can storytelling be used in college English classes to help students improve their communicative skills? In what aspects can Korean college students, in particular, benefit from storytelling? What are some other benefits of storytelling as a teaching strategy for college students? The sample consisted of 51 students from a mid-sized private university in Kangwon Province of South Korea. 26 students from Storytelling Class and 25 from College English Class were selected for this study. All of them were aged between 19 and 20, and they had been identified as English Level 2 students by the university based on their initial TOEIC scores at the entrance to the university. This study applied a mixed-approach to gathering two kinds of data – quantitative and qualitative. The quantitative data was collected from TOEIC tests and 10-minute writing tests, while the qualitative data was collected from observations of students’ behavior during class activities and performances and documentation of their writing samples from the10-minute writing tests. The data analyses revealed that storytelling was a significantly effective teaching strategy for college students in improving TOEIC Reading Comprehension (RC) score and writing skills in general. The results of the data analyses also supported that storytelling was especially effective in teaching students to become more creative, open-mined, logical, and critical writers. In addition, the students who learned English through storytelling showed an improvement in problem-solving skills and teamwork in this study. However, the study results did not provide enough supports to suggest that storytelling would be a more effective teaching strategy for college English education as compared to another typical teaching strategy that is currently used in college English courses. After the analyses of the data, this study supported the following conclusions. First, integrating storytelling lessons in college English education may be an effective teaching strategy. However, additional research should be conducted in order conclusively prove its effectiveness. Nevertheless, the researcher hoped that this study could provide a platform for the college English teachers so that they would be encouraged to use storytelling as a more effective way of teaching English and how they should go about designing their future lessons.

Characterizations of the Antarctic Sea Ice Using Multiple Satellite Remote Sensing Data

Miae Kim Graduate School of UNIST 2018 국내박사

Sea ice closely interacts with the atmosphere and ocean systems. Land fast sea ice (fast ice) is a kind of sea ice attached to the shore, ice shelves, or grounded icebergs. It is widely distributed along the Antarctic coast and acts as an interface between the atmosphere and the ocean, affecting heat balance feedback, thermal insulation effects, and deep water formation depending on the temporal and spatial effects of the environmental conditions. It also plays an important role in the biological aspects of Antarctica. Attached to the Antarctic glacier is strongly associated with calving events of ice shelf as it is physically coupled with glaciers at the terminus. The existing Antarctic fast ice has been mainly focused on the East Antarctic, especially for the research on long-term fast ice. Several case studies for West Antarctic fast ice with satellite images were performed in local areas. Various types of satellite data and detection techniques were utilized to successfully detect fast ice. In addition, long-term fast ice maps specifically focused on the Amundsen sea of West Antarctica were generated to investigate the distribution and variability of fast ice. This thesis reports the results of fast ice detection algorithms that have been developed using various satellite images that can be used for fast ice detection. Along with the use of multiple satellite data, the proposed fast ice detection algorithms can more effectively detect fast ice, which then allows to obtain more accurate fast ice detection and produce long-term fast ice with high accuracy. Especially, the distribution and variability of time-series fast ice in West Antarctica, which is more concentrated in the Amundsen Sea, were analyzed together with bathymetry data and the distribution of glacier icebergs. In order to detect fast ice, machine learning techniques were basically used in this thesis. Two classes (i.e. fast ice and non-fast ice) were classified. Using MODIS images, there was a problem that fast ice was not produced in cloud cover areas and the polar night season, which is winter season in Antarctica. MODIS and AMSR-E satellite data were selectively used to solve the cloud contamination problem. Correlation-related variables were finally added based on the fact that fast ice is motionless for a certain period of time, and fast ice detection was performed at 15-day intervals using the improved input variables. Active microwave sensor data, ALOS PALSAR, was also used to detect fast ice and to validate fast ice detection results. Its high-spatial resolution allows to extract fast ice boundary more accurately. Fast ice detections showed good agreement with available ALOS PALSAR SAR images and MODIS reflectance images. Nearly decade-long fast ice extents were produced in the Amundsen Sea of West Antarctica and analyzed in terms of spatiotemporal variations with bathymetry and icebergs calved from ice shelves in study area. In addition, anomalous fast ice breakup events were examined, which suggests the importance of fast ice on the stability of ice shelves.

(The) regulatory mechanism of the proteasome-dependent degradation of human cancer-upregulated gene 2 (CUG2)

이미애 충남대학교 대학원 2009 국내석사

We previously examined genome-wide differences in gene expression between tumor biopsies and normal tissues in order to identify differentially regulated genes in tumors. Cancer upregulated gene 2 (CUG2) was identified as a gene that exhibits significant differential expression in multiple human cancer types. CUG2-overexpressing mouse fibroblast cells exhibited distinct cancer-specific phenotypes in vitro and developed into tumors in nude mice, suggesting that CUG2 is a novel tumor-associated gene. CUG2 has been shown to localize to the nucleolus. This was confirmed by its colocalization with B23, the nucleolar protein. And, we confirmed CUG2 is co-localization with B23, CENP-T, ACA in mammalian cells, and GST pulldown experiments demonstrate clearly a physical interaction between CUG2 and the CENP-T or CENP-A. GST pulldowns identified CENP-T or CENP-A for CUG2 interaction. These results suggest that CUG2 localize to centromeres in mammalian cell. In addition, CUG2 can bind to CSN5. CSN5 plays a pivotal role in cancer formation, and is aberrantly over expressed in various types of cancer. This oncogenic activity may arise mostly from the elevated activity in degrading important regulators involved in cell proliferation control. Thus, CSN5 can be an important molecular target for rational cancer therapy. We have shown here that CSN5 interacts with the CUG2 protein in mammalian cells. As expected, over expression of CSN5 is accompanied by stabilization of CUG2 protein. We demonstrated that CSN5 induces mediates its degradation via the 26 S proteosome pathway. It is shown in ubiquitination assays and by the proteasome inhibitor MG132 that CUG2 is a substrate of the UPS. Under normal cell conditions, CUG2 is stable with a half-life of about 1 h. This stability is increasing after downregulating the amount of the CSN5 in cells or after treatment of cells with curcumin, an inhibitor of CSN-associated kinases. In this study, we provide evidence for the involvement of the CSN in the regulation of CUG2 degradation via the Ub pathway, indicating decelerated degradation via the Ub pathway in the presence of CENP-T.

Lysosomal signaling by soraprazan promotes longevity in Caenorhabditis elegans through adjusting peroxisomal beta-oxidation

오미애 Greduate School, Korea University 2021 국내석사

Stargardt disease and age-related macular degeneration (AMD) induce the accumulation of lipofuscin in retinal pigment epithelium (RPE) cells, causing loss of vision. Soraprazan is developed to treat these diseases by removing lipofuscin from RPE cells. Lipofuscin is also a representative aging biomarker, as generated naturally through metabolism. However, lifespan extension effect of soraprazan is not known. In this study, longevity effect of soraprazan in Caenorhabditis elegans was investigated and its mechanism was predicted. Survival rate of C. elegans and aging biomarkers (body length, pharyngeal pumping rate, lipofuscin, and reactive oxygen species (ROS)) were measured in soraprazan-treated C. elegans. Microarray analysis was performed to evaluate the change of mRNA expression patterns in soraprazan-treated C. elegans and the negative control. Gene expression was measured by quantitative real-time polymerase chain reaction (qRT-PCR). To elucidate the mechanism of lifespan extension in soraprazan-treated C. elegans, loss-of-function mutants of C. elegans were used. The results showed that soraprazan significantly (p < 0.05) extended the lifespan of C. elegans (N2) compared with the negative control. Body length was reduced in soraprazan-treated C. elegans. Pharyngeal pumping rate was decreased by treatment of soraprazan, which might induce the dietary restriction like (DR-like) state despite of enough food. Lipofuscin accumulation and reactive oxygen species (ROS) were reduced dose-dependently in soraprazan-treated C. elegans. Microarray analysis and qRT-PCR results showed the increase of expression levels of the genes related to lysosome (lipl-1 and lbp-8), nuclear hormone receptors (nhr-210 and nhr-234), peroxisomal fatty acid beta-oxidation (acox-3 and ech-9), and xeonobiotic detoxification (cyp-34A1, cyp-35A1, cyp-35A2, cyp-35A3, cyp-35A4, cyp-35A5, cyp-35C1, gst-28, and gst-5). Moreover, loss-of-function mutants (lipl-1, lbp-8, nhr-234, nhr-49, nhr-8, cyp-35A1, cyp-35A2, cyp-35A3, cyp-35A5, and gst-5) failed to extend the lifespan in soraprazan-treated C. elegans, which suggests that these genes might be engaged with the lifespan extension in soraprazan-treated C. elegans. Nhr-234 deleted mutant (VC1806) treated with soraprazan did not increase expression levels of acox-3, ech-9, cyp-35A1, cyp-35A2, cyp-35A3, and cyp-35A4 compared with the control (N2) treated with soraprazan, which suggests that nhr-234 might regulate these genes resulted in lifespan extension by soraprazan in C. elegans. In conclusion, soraprazan extends the lifespan of C. elegans through increasing expression level of lysosomal lipases followed by adjusting peroxisomal beta-oxidation.

Characterizations of the antarctic sea ice using multiple satellite remote sensing data

김미애 UNIST 2018 국내박사

Effects of Cultural values and behaviors in Sociocultural stress and coping among Korean-American and Korean Caregivers

전미애 University of Southern California 2004 해외박사

Identification of the function and regulatory network of AP5M1 as a putative tumor suppressor

원미애 CHA University 2017 국내박사

AP5M1 (adaptor related protein complex 5 mu 1 subunit) was initially discovered as a Fas-mediated pro-death gene after screening of a randomized hybrid-ribozyme library. AP5M1 is an evolutionally conserved protein in diverse species including dog, cow, rat, mouse, chicken, zebrafish and xeopus with ubiquitous expression, suggesting that may have a common function in cell death. The current study demonstrated that AP5M1 functions as a cell death inducer in HeLa cells and Jurket T cells. For many years, it had been assumed that only four adaptor protein (AP) complexes existed until the recent discovery of the AP-5 complex. Four AP complexes (AP-1 to AP-4) localize to different regions of the cell, bind and sort specific cargo proteins between distinctive intracellular membrane compartments. However, no direct evidence has been published regarding that the AP-5 complex indeed functions to regulate endocytic trafficking, and no candidate cargo proteins have been identified. In this study, I discovered the trafficking pathway of AP-5 for the first time by identifying the tumor necrosis factor (TNF) receptor FAS as the cargo protein of the AP-5 complex. I demonstrated that AP5M1 is an essential molecule for mediating the endocytic vesicular trafficking of FAS. More importantly, AP5M1-mediated FAS internalization is absolutely required for inducing the apoptotic activity of FAS in cervical carcinoma cells. Furthermore, human cervical carcinomas express negligible levels of AP5M1, whereas noncancerous cervical epitheliums express abundant AP5M1. The delivery of the antisense oligonucleotides of AP5M1 promotes cervical tumor growth and prevents the FAS-induced repression of tumor growth in vivo in xenografted mice. My findings indicate that AP5M1 is a putative tumor suppressor that is pivotal for regulating FAS-induced apoptosis by controlling receptor endocytosis. Furthermore, a previously unidentified pathophysiological mechanism that may account for the malignancy of cervical carcinoma is provided in this study; specifically, defective FAS-mediated cell death signaling resulted from AP5M1 depletion. AP5M1(adaptor related protein complex 5 mu 1 subunit)는 randomized hybrid-ribozyme 라이브러리 스크리닝을 과정을 통해, Fas 매개 된 세포사멸 유전자로서 처음 발견되었다. AP5M1 단백질은 개, 암소, 쥐, 닭, 지브라 피쉬, 개구리 등을 포함한 다양한 종에서 발현되고 있으며 진화적으로 보존된 단백질로서, 세포 사멸에 있어서도 공통적인 기능을 가지고 있음을 시사한다. 현재까지 연구에서 AP5M1 단백질은 HeLa 세포 및 Jurket T 세포에서 세포 사멸 유도체로서의 기능을 가지고 있음을 입증하였다. 수년 동안, 최근 연구를 통한 AP-5 복합체의 발견 이전까지, APs (adaptor proteins)은 4(AP-1~AP-4)개의 복합체들만이 존재한다고 추정되어 왔다. 4개의 AP 복합체들은 세포의 각기 다른 영역에 존재하고 있으며, 특유의 세포 내 막 구획 사이에서 특정 cargo 단백질과 결합하거나 이동한다. 그러나 AP-5 복합체는 실제적인 endocytotic trafficking을 조절하는 작용기전에 대한 직접적인 증거가 없으며, 후보화 된 cargo 단백질도 확인 되지 않았다. 이번 연구를 통해, TNF (tumor necrosis factor) 수용체인 FAS 단백질 AP-5 복합체의 cargo 단백질임을 확인함으로서, AP-5 복합체의 이동 조절기작을 처음 발견하였다. AP5M1이 FAS의 endocytic vesicular trafficking을 매개하는 필수 분자임을 증명하였다. 더욱 중요한 것은, 자궁 경부암 세포에서 FAS의 세포 사멸 활성을 유도하기 위해서는 AP5M1이 매개된 FAS의 internalization이 절대적으로 필요하다는 것이다. 더 나아가, 인간의 자궁 경부암 조직에서 AP5M1 단백질의 발현이 현저하게 낮은 수준으로 발현되었고, 정상 자궁 경부 상피 조직에서는 풍부하게 많은 양의 AP5M1의 발현이 확인 되었다. 또한 이종이식 마우스 모델에서 안티센스 올리고뉴클레오타이드 (antisense oligonucleotides)의 전달을 통한 AP5M1 단백질은 자궁 경부 종양의 성장을 촉진시키며, 생체 내에서 FAS에 의한 사포 사멸 유도를 방지하였다. 본 연구 결과에서, AP5M1 단백질은 수용체 endocytosis를 조절하여 FAS 유도 된 세포 사멸을 조절하여 종양억제제로의 기능이 추정됨을 알 수 있었다. 또한, 본 연구는 구체적으로 AP5M1 결여로 인한 FAS 매개된 세포 사멸 조절기전 결함을 통해 자궁 경부암의 악성을 설명할 수 있는 이전의 확인되지 않았던 병태 생리학전 기전 연구에 제공되어 질 수 있다.

Fabrication of waste forms to immobilize lanthanide wastes generated from pyrochemical processing and its dissolution behavior

김미애 Pohang University of Science and Technology 2017 국내박사

A study on effectiveness by psychological therapy of church music

장미애 The Open International University for Alternative 2000 해외박사