녹용 물 추출액이 난소절제 흰쥐의 혈청 중 성 호르몬 함량 변화에 미치는 영향

김미려,양재하,권용준 한국노화학회 1998 한국노화학회지 Vol.8 No.3

녹용 물 추출액이 난소절제 흰쥐의 골대사에 미치는 영향

김미려,양재하,권용준 한국노화학회 1998 한국노화학회지 Vol.8 No.3

난소를 절제한 흰쥐의 대퇴골에 미치는 녹용 물 추출액의 영향

김미려,양재하 동서의학사 1998 동서의학 Vol.23 No.1

침자극이 난소를 절제한 흰쥐의 혈청 중 성 hormone 함량에 미치는 영향

김미려,양재하 慶山大學校 基礎科學硏究所 1998 基礎科學 Vol.2 No.1

흰주의 난소를 절제하여 골다공증을 유발한 뒤, 4주 동안 부인과 질환과 근골계질환의 치료에 쓰고 있는 삼음교(SP6)혈과 양릉천(GB34)혈을 침자극하여 혈청 중 성 hormone 함량 변화에 대한 영향을 살펴보았다. 난소절제 후에 혈청 중 estradiol, androstenedione, testosterone 함량이 유의하게 감소하는데, 난소절제 후에 삼음교(SP6)혈과 양릉천(GB34)혈을 침자극하면 혈청 중 androstenedione, testosterone 함량이 난소절제군보다 유의하게 증가되었으며, estradiol의 함량도 증가되었다. 삼음교(SP6)혈과 양릉청(GB34)의 침자극은 난소를 절제한 흰쥐에서 골대사와 관련이 있는 혈중 estrogen 및 전구체인 androstenedione, testosterone 함량을 증가 시킴으로써 난소절제 후, 또는 폐경 후 골다공증의 개선제로 이용될 수 있을 것으로 생각된다. The effect of acupuncture at Sanyinjiao(SP6) and Yanglingquan(GB34) on serum levels of sex hormones was investigated in ovariectomized rats. An elevation of androstenedione, testosterone and estradiol was elecited in ovariectomized rats after 2 and 4 weeks of acupncture treatment, respectively. These results show the possibility that acupncture can play a role of ameliorating osteoporosis by elevating serum levels of sex hormones related with bone metabolism.

구자가 卵巢摘出로 誘發된 흰쥐의 骨多孔症에 미치는 影響 (2)

徐富一,金先熙,卞晟僖,金美麗 대한본초학회 1998 大韓本草學會誌 Vol.13 No.2

This study was performed to investigate the effects of Allii Tuberosi Semen(菲子) on osteoporosis after ovariectomy in rats. Control group received a daily po administration of normal saline for 4 weeks after oophorectomy(OVX). The other experimental group received a daily po administration of water extract of Allii Tuberosi Semen for 4 weeks after oophorectomy(OVX-ATS). Therefore, we measured levels of calcium, phosphorus, ash weights in femur. These results were as follows : 1. In the level of bone phosphorus, the result of OVX was 63.85±1.86㎎/㎗, but the result of OVX-ATS was 68.18±0.85㎎/㎗. But there was no statistical significance. 2. In the level of bone calcium, the result of OVX was 141.66±2.19㎎/㎗, but the result of OVX-ATS group was 164.82±3.74㎎/㎗. And the result of OVX-ATS showed significant increase in comparison with OVX. 3. In the level of bone ash weight, the result of OVX was 0.31±0.005(g), but the result of OVX-ATS was 0.33±0.002(g). And the result of OVX-ATS showed significant increase in comparison with OVX. The observations from these studies and the past studies(=A study on the effects of Allii Tuberosi Semen in ovariectomized osteoporosis of rats) suggest possible protective effect of Alli Tuberosi Semen water extract against bone loss in ovariectomized rats.

Carbon tetrachloride 투여로 인한 Rat의 肝毒性에 미치는 Methionine의 影響

권용준,조경희,김미려 대구효성가톨릭대학교 1994 연구논문집 Vol.49 No.1

The GxSxG motif of Arabidopsis monoacylglycerol lipase (MAGL6 and MAGL8) is essential for their enzyme activities

Kim, Ryeo Jin,Suh, Mi Chung The Korean Society for Applied Biological Chemistr 2016 Applied Biological Chemistry (Appl Biol Chem) Vol.59 No.6

Monoacylglycerol lipase (MAGL) catalyzes the hydrolysis of monoacylglycerols (MAG) to free fatty acids and glycerol, which is the last step of triacylglycerol breakdown. Among sixteen members, Arabidopsis thaliana MAGL6 (AtMAGL6) and AtMAGL8 showed strong lipase activities, but several AtMAGLs including AtMAGL16 displayed very weak activities (Kim et al. in Plant. J 85:758-771, 2016). To understand the internal factors that influence Arabidopsis MAGL activities, this study investigated the significance of 'GxSxS motif,' which is conserved in MAGLs. First, we observed that the presence of a serine protease inhibitor, phenylmethylsulfonyl fluoride, decreased the enzyme activity of AtMAGL6 and AtMAGL8 by $IC_{50}$ values of 2.30 and 2.35, respectively. Computational modeling showed that amino acid changes of the GxSxG motif in AtMAGL6 and AtMAGL8 altered the nucleophilic elbow structure, which is the active site of MAGLs. Mutating the GxSxG motif in the recombinant maltose binding protein (MBP):AtMAGL6 and MBP:AtMAGL8 proteins to SxSxG, GxAxG, and GxSxS motifs completely demolished the activities of the mutant MAGLs. In contrast, no significant differences were observed between the activities of AtMAGL16 wild type form harboring the SxSxG motif, and mutant AtMAGL16 containing the GxSxG motif. These results revealed that the glycine and serine residues of the GxSxG motif are essential for AtMAGL6 and AtMAGL8 enzyme activities, and that AtMAGL16 may not be involved in the hydrolysis of lipid substrates.

Development of camelina enhanced with drought stress resistance and seed oil production by co-overexpression of MYB96A and DGAT1C

Kim, Ryeo Jin,Kim, Hyun Uk,Suh, Mi Chung Elsevier 2019 INDUSTRIAL CROPS AND PRODUCTS Vol.138 No.-

<P><B>Abstract</B></P> <P>Camelina (<I>Camelina sativa,</I> Cs) is an emerging crop for the production of biodiesel and biofeedstock. This study aims to develop transgenic plants that have the advantage of two traits: improved drought resistance and oil content. Three genes, <I>CsMYB96A</I>, <I>CsMYB96B</I>, and <I>CsMYB96C,</I> were isolated from camelina stem. The deduced amino acid sequence of the three CsMYB96s showed at least 93% identity with Arabidopsis MYB96. <I>CsMYB96A</I>, <I>B,</I> and <I>C</I> transcripts were detected in various camelina tissues. Fluorescence signal from the fusion of CsMYB96A: enhanced yellow fluorescent protein was confined to the nucleus of tobacco epidermal cells. Transactivation analysis of tobacco protoplasts revealed that <I>CsMYB96A</I> was a transcription activator. Wax biosynthesis genes such as camelina <I>β-ketoacyl-CoA synthase 2</I>, <I>β-ketoacyl-CoA synthase 6</I>, <I>β-ketoacyl-CoA reductase 1-1</I>, <I>β-ketoacyl-CoA reductase 1-2</I>, <I>enoyl-CoA reductase</I>, <I>ECERIFERUM 1</I> and <I>ECERIFERUM 3</I> were upregulated approximately 2 to 120 times by CsMYB96A, indicating that CsMYB96A was involved in the activating of cuticular wax synthesis on plant epidemics. Camelina diacylglycerol acyltransferase 1C (CsDGAT1C) has been shown to increased oil synthesis genes in Arabidopsis. When <I>CsDGAT1C</I> and <I>CsMYB96A</I> were co-overexpressed in camelina, total fatty acid levels in transgenic seeds increased by approximately 21%. In addition, the transgenic camelina plants showed improved resistance to drought stress. This result suggests that the transgenic camelina can be grown to produce biodiesel and biofeedstock in arid or semi-arid lands.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Three copies of the MYB96 transcription factor genes were isolated from camelina. </LI> <LI> Camelina MYB96A targeted to nucleus and showed transcriptional activation. </LI> <LI> Co-overexpression of CsMYB96A and CsDGAT1C promoted triacylglycerol synthesis in camelina seeds. </LI> <LI> Co-overexpression of CsMYB96A and CsDGAT1C in camelina enhanced drought stress resistance. </LI> </UL> </P>

Expression Pattern of Entire Cytochrome P450 Genes and Response of Defensomes in the Benzo[<i>a</i>]pyrene-Exposed Monogonont Rotifer <i>Brachionus koreanus</i>

Kim, Ryeo-Ok,Kim, Bo-Mi,Jeong, Chang-Bum,Nelson, David R.,Lee, Jae-Seong,Rhee, Jae-Sung American Chemical Society 2013 Environmental science & technology Vol.47 No.23

<P>Cytochrome P450 (<I>CYP</I>) proteins are involved in the first line of detoxification mechanism against diverse polycyclic aromatic hydrocarbons (PAHs) including benzo[<I>a</I>]pyrene (B[<I>a</I>]P). In aquatic invertebrates, there is still a lack of knowledge on the <I>CYP</I> genes involved in the molecular response to B[<I>a</I>]P exposure due to limited gene information. In this study, we cloned the entire 25 <I>CYP</I> genes in the monogonont rotifer Brachionus koreanus with the aid of next generation sequencing (NGS) technologies and analyzed their transcript profiles with a real-time RT-PCR array to better understand B[<I>a</I>]P-triggered molecular response over different time courses. As a result, B[<I>a</I>]P exposure induced <I>CYP2</I>/<I>3</I>-involved detoxification mechanisms and defensome, including phase II detoxification and antioxidant systems with a modulation of the chaperone heat shock protein (<I>hsp</I>) expression but did not change expression of other <I>CYP</I> clans in B. koreanus. Therefore, we found that B[<I>a</I>]P induced a strong detoxification mechanism to overcome detrimental effects of B[<I>a</I>]P associated with B[<I>a</I>]P-induced growth retardation as a trade-off in fitness costs. Also, this approach revealed that the entire <I>CYP</I> profiling can be a way of providing a better understanding on the mode of action of B[<I>a</I>]P in B. koreanus with respect to molecular defense metabolism.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/esthag/2013/esthag.2013.47.issue-23/es403269v/production/images/medium/es-2013-03269v_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/es403269v'>ACS Electronic Supporting Info</A></P>

Development of a monoclonal antibody against glucosyltransferase D of Streptococcus mutans GS 5.

Kim, Mi-Ah,Yang, Yeon-Mi,So, Yu-Ryeo,Ko, Young-Han,Lim, Su-Min,Lee, Kyung-Yeol,Kim, Jae-Gon Mary Ann Liebert, Inc 2011 Hybridoma Vol.30 No.4

<P>Glucosyltransferases GtfB, GtfC, and GtfD of Streptococcus mutans are virulent factors involved in dental caries. Consequently, they are considered to be target molecules in the development of vaccines against dental caries. Among them, GtfD plays a significant role in the sucrose-dependent cellular adhesion of S. mutans, and a number of studies have suggested that the N-terminus of GtfD is an important part of its role in enzymatic activity. In this study, we generated monoclonal antibodies against the N-terminus of GtfD (anti-GtfDN antibody) in an initial attempt to investigate its preventive efficacy against dental caries. To obtain anti-GtfDN monoclonal antibodies, the gene for the N-terminus of gtfD (2?kb) was cloned into an Escherichia coli expression vector, pQE30; then the expressed protein (about 75?kDa) was purified. The purified GtfDN protein was injected into BALB/c mice, and hybridoma clones were established. We obtained three hybridoma clones (HDN9, HDN11, and HDN28) capable of producing anti-GtfDN antibodies. Their binding specificity was characterized by ELISA, dot blot, and Western blot analysis after purification using affinity column chromatography. The isotype of the monoclonal antibodies was confirmed to be IgG2a.</P>