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1
What do patients with urothelial cancer know about the association of their tumor disease with smoking habits? Results of a German survey study

Matthias May,Hans-Martin Fritsche,Christian Gilfrich,Mirja Dombrowski,Odilo Maurer,Philipp Spachmann,Manju Ganesh Kumar,Marc Bjurlin,Maximilian Burger,Sabine Brookman-May 대한비뇨의학회 2018 Investigative and Clinical Urology Vol.59 No.3
Purpose: Smoking represents a primary risk factor for the development of urothelial carcinoma (UC) and a relevant factor impacting UC-specific prognosis. Data on the accordant knowledge of UC-patients in this regard and the significance of physicians in the education of UC-patients is limited. Materials and Methods: Eighty-eight UC-patients were enrolled in a 23-items-survey-study aimed to analyse patient knowledge and awareness of their tumor disease with smoking along with physician smoking cessation counselling. Results: The median age of the study patients was 69 years; 26.1% (n=23), 46.6% (n=41), and 27.3% (n=24), respectively, were non-smokers, previous, and active smokers. Exactly 50% of active smokers reported a previous communication with a physician about the association of smoking and their tumor disease; however, only 25.0% were aware of smoking as main risk factor for UC development. Merely 33% of the active smokers had been prompted directly by their physicians to quit smoking. About 42% of active smokers had received the information that maintaining smoking could result in a tumor-specific impairment of their prognosis. Closely 29% of active and about 5% of previous smokers (during the time-period of active smoking) had been offered support from physicians for smoking cessation. No association was found between smoking anamnesis (p=0.574) and pack-years (p=0.912), respectively, and tumor stage of UC. Conclusions: The results of this study suggest that the medical conversation of physicians with UC-patients about the adverse significance of smoking is limited. Implementation of structured educational programs for smoking cessation may be an opportunity to further enhance comprehensive cancer care.
2
In situ spectroscopy during As-modification of Si(100) and single-domain GaP heteroepitaxy for III/V-on-Si tandem absorbers

Oliver SUPPLIE,Matthias M. MAY,Agnieszka PASZUK,Andreas NAEGELEIN,Peter KLEINSCHMIDT,Sebastian BRUECKNER,Thomas HANNAPPEL 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8
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3
Interference of engineered nanomaterials in flow cytometry: A case study

Bohmer, Nils,Rippl, Alexandra,May, Sarah,Walter, Auré,lie,Heo, Min Beom,Kwak, Minjeong,Roesslein, Matthias,Song, Nam Woong,Wick, Peter,Hirsch, Cordula Elsevier 2018 Colloids and Surfaces B Vol.172 No.-
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Abstract Nanotechnology is regarded as the enabling technology of the 21st century. However, only a relatively small number of nano-enabled medical and healthcare products finally made their way to the market. There are several reasons why such innovative approaches fail in translation, with one key factor being the uncertainty surrounding their safety assessment. Although well described, interference reactions of engineered nanomaterials (ENM) with classical cytotoxicity assays remain a major source of uncertainty. Flow cytometry is a powerful, widely used, in vitro technique. Its readout is based on the detection of refracted laser light and fluorescence signals. It is therefore susceptible to ENM interference. Here we investigated possible interferences of ENM in the Annexin V/propidium iodide (PI) assay, which quantifies apoptotic and necrotic cell populations by flow cytometry. Two case studies were conducted using either silica or gold nanoparticles differing in size, specific surface area and surface chemistry. Both ENM types were found to cause distinct interference reactions at realistic concentrations. Silica particles induced false-positive signals; however only in the absence of a protein corona and in conjunction with a particular fluorophore combination (FITC/PI). In contrast, gold particles led to complex quenching effects which were only marginally influenced by the presence of proteins and occurred for both fluorophore combinations analyzed. We present a versatile spike-in approach which is applicable to all ENM and cell types. It further allows for the identification of a broad range of different interference phenomena, thereby increasing the reliability and quality of flow cytometry and ENM hazard assessment. Highlights <UL> <LI> Silica nanoparticles interfere with propidium iodide in the AnnexinV/PI assay. </LI> <LI> Serum proteins prevent interference of silica nanoparticles. </LI> <LI> Gold nanoparticles quench fluorescence in dependence of their surface chemistry. </LI> <LI> Special designed spike-in controls enable detection of interferences. </LI> </UL> Graphical abstract [DISPLAY OMISSION]