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Mussel foot protein-1 (mcfp-1) interaction with titania surfaces
Hwang, Dong Soo,Harrington, Matthew J.,Lu, Qingye,Masic, Admir,Zeng, Hongbo,Waite, J. Herbert The Royal Society of Chemistry 2012 Journal of materials chemistry Vol.22 No.31
<P>Marine mussels utilize a variety of DOPA-rich proteins for purposes of underwater adhesion, as well as for creating hard and flexible surface coatings for their tough and stretchy byssal fibers. In the present study, moderately strong, yet reversible wet adhesion between the protective mussel coating protein, mcfp-1, and amorphous titania was measured with a surface force apparatus (SFA). In parallel, resonance Raman spectroscopy was employed to identify the presence of bidentate DOPA–Ti coordination bonds at the TiO<SUB>2</SUB>–protein interface, suggesting that catechol–TiO<SUB>2</SUB> complexation contributes to the observed reversible wet adhesion. These results have important implications for the design of protective coatings on TiO<SUB>2</SUB>.</P> <P>Graphic Abstract</P><P>Moderately strong, yet reversible wet adhesion between mussel coating protein and titania was measured with a SFA and Raman spectroscopy. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2jm32439c'> </P>
Kim, Bum Jin,Kim, Sangsik,Oh, Dongyeop X.,Masic, Admir,Cha, Hyung Joon,Hwang, Dong Soo The Royal Society of Chemistry 2015 Journal of Materials Chemistry B Vol.3 No.1
<P>Marine mussels utilize multiple bidentate complexes formed by Fe(iii) and DOPA in a mussel adhesive protein (fp-1) to reinforce tough and elastic byssal fibers as a specialized underwater adhesive aid. In this study, mussel-inspired electrospun nanofibers were fabricated using a recombinant mussel adhesive protein (<I>r</I>fp-1), Fe(iii)-DOPA complexes, and polycaprolactone. The mechanical properties of the fabricated nanofibers were reinforced by the Fe(iii)-DOPA complex found in fp-1, which is a key component of the naturally occurring high-performance mussel fiber coating. Experimental results show that the stoichiometry of Fe(iii)-DOPA complexes in the nanofibers could be controlled by buffer pH conditions and the stiffness of the nanofiber mat increased linearly with the concentration of the Fe(iii)-DOPA complexes, as monitored by resonance Raman spectroscopy. This suggests the potential of Fe(iii)-DOPA complexation as an effective strategy for modulating the mechanical properties of nanofibrous biomedical materials by using pH variations.</P>
Mussel-Mimetic Protein-Based Adhesive Hydrogel
Kim, Bum Jin,Oh, Dongyeop X.,Kim, Sangsik,Seo, Jeong Hyun,Hwang, Dong Soo,Masic, Admir,Han, Dong Keun,Cha, Hyung Joon American Chemical Society 2014 Biomacromolecules Vol.15 No.5
<P>Hydrogel systems based on cross-linked polymeric materials which could provide both adhesion and cohesion in wet environment have been considered as a promising formulation of tissue adhesives. Inspired by marine mussel adhesion, many researchers have tried to exploit the 3,4-dihydroxyphenylalanine (DOPA) molecule as a cross-linking mediator of synthetic polymer-based hydrogels which is known to be able to achieve cohesive hardening as well as adhesive bonding with diverse surfaces. Beside DOPA residue, composition of other amino acid residues and structure of mussel adhesive proteins (MAPs) have also been considered important elements for mussel adhesion. Herein, we represent a novel protein-based hydrogel system using DOPA-containing recombinant MAP. Gelation can be achieved using both oxdiation-induced DOPA quinone-mediated covalent and Fe<SUP>3+</SUP>-mediated coordinative noncovalent cross-linking. Fe<SUP>3+</SUP>-mediated hydrogels show deformable and self-healing viscoelastic behavior in rheological analysis, which is also well-reflected in bulk adhesion strength measurement. Quinone-mediated hydrogel has higher cohesive strength and can provide sufficient gelation time for easier handling. Collectively, our newly developed MAP hydrogel can potentially be used as tissue adhesive and sealant for future applications.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bomaf6/2014/bomaf6.2014.15.issue-5/bm4017308/production/images/medium/bm-2013-017308_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/bm4017308'>ACS Electronic Supporting Info</A></P>