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RISS 인기검색어
- 검색키워드
- 저자 : Masawo Kamanoi(釜野井 定男) (검색결과 2 건)
- 무료
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듀럼밀 三染色體 植物에서 剩餘染色體의 細胞學的 行動 및 傳達率
Sea kwan Oh(吳世寬),Masawo Kamanoi(釜野井 定男) 한국육종학회 1997 한국육종학회지 Vol.29 No.1
At first metaphase of PMCs in the trisomics of durum wheat(Triticum durum var. hordeiforme), various configurations of chromosomes with varying number of trivalents representing V shape(75.45%), Frying-pan shape(16.7%), Chain shape(7.06%) and Y shape(0.71%) were observed. The frequency of chromosome configuration of 13Ⅱ+1Ⅲ at first metaphase except for Tri-2B line appeared much less than that of 14Ⅱ+1Ⅰ, which showed the percentage of average number of the 13 trisomics was 45.35% while 14Ⅱ+1Ⅰ showed 52.48%. At first anaphase, chromosomes were separated to 14 : 15 type (14Ⅱ: 13Ⅱ+1Ⅲ) in many sporocytes, while one lagging chromosome (14Ⅱ:1Ⅰ: 14Ⅱ) was observed frequently. Among the selfed progeny of homozygous trisomics, the lowest transmission of extra chromosome was observed in Tri-4A line (15.0%) and the highest in Tri-2B line(40.0%), and the average transmission rate of the trisomic lines was 26.9%. While the extra chromosome was transmitted in all types, the frequency of transmission of the extra chromosome through the pollen had been found to be considerably less than that of the eggs. In either case, the reduction in the transmission rate of the extra chromosome was suspected to be due to the loss of univalent chromosome and functional depression in the aneuploidy gamate at fertilization.
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마카로니(durum)밀의 三染色體植物을 이용한 esterase isozyme 遺傳子의 染色體位置 同定
Sea Kwan Oh(吳世寬),Masawo Kamanoi(釜野井 正男) 한국육종학회 1995 한국육종학회지 Vol.27 No.3
This study was carried out for identification of esterase isozyme polymorphism which may be an useful way to understand the physiological and biochemical characteristics in durum wheat, Triticum durm var. hordeiforme(2n=4x=28 AABB). The esterase isozyme was detected by electrophoretic separation and activity staining techniques with the endosperm sample from germinated durum wheat grain. Artifical octoploid showed nine isozyme bands which were designated as Ei-1, Ei-2, Ei-3, Ei-4, Ei-5, Ei6, Ei-7, Ei-8 and Ei-9 from cathode side. Normal tetraploid plant(2n=4x=28) showed six bands, without bands of Ei-6, Ei-8 and Ei-9 in durum wheat. Only six bands such as Ei-1, Ei-2, Ei-3, Ei-4, Ei-5 and Ei-7 was detected in Tri-1A(2n=4x=29 AABB+11A) line, but the activity of isozyme in Tri-1A line was above double one of normal plants. Therefore, the gene(s) controlling six bands of esterase isozyme was considered to be located on the chromosome 1A. Moreover, the activity of six esterase isozyme bands in Tri-1B(2n=4x=29 AABB+11B) line was same as normal plants, but nine bands including Ei-6, Ei-8 and Ei-9 were detected in esterase isozyme of Tri-1B line. These three bands appeared in artifical octoploid(2n=8x=56 AAAABBBB) and did not appeared in normal plants or other trisomic lines, except Tri-1B line. Consequently, the gene controlling these three bands was considered to be on the chromosome 1B.
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