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RISS 인기검색어
- 검색키워드
- 저자 : Masami Tanio (검색결과 2 건)
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Aoyagi, Yasuyuki,Kuroda, Masayuki,Asada, Sakiyo,Bujo, Hideaki,Tanaka, Shigeaki,Konno, Shunichi,Tanio, Masami,Ishii, Itsuko,Aso, Masayuki,Saito, Yasushi Korean Society for Biochemistry and Molecular Bion 2011 Experimental and molecular medicine Vol.43 No.3
The development of clinically applicable scaffolds is important for the application of cell transplantation in various human diseases. The aims of this study are to evaluate fibrin glue in a novel protein replacement therapy using proliferative adipocytes and to develop a mouse model system to monitor the delivery of the transgene product into the blood and the fate of the transduced cells after transplantation. Proliferative adipocytes from mouse adipose tissue were transduced by a retroviral vector harboring the human lecithin- cholesterol acyltransferase ($lcat$) gene, and were subcutaneously transplanted into mice combined with fibrin glue. The $lcat$ gene transduction efficiency and the subsequent secretion of the product in mouse adipocytes were enhanced using a protamine concentration of $500{\mu}g/ml$. Adipogenesis induction did not significantly affect the $lcat$ gene-transduced cell survival after transplantation. Immunohistochemistry showed the ectopic enzyme production to persist for 28 days in the subcutaneously transplanted genetransduced adipocytes. The increased viability of transplanted cells with fibrin glue was accompanied with the decrease in apoptotic cell death. The immunodetectable serum LCAT levels in mice implanted with the fibrin glue were comparable with those observed in mice implanted with Matrigel, indicating that the transplanted $lcat$ gene-transduced adipocytes survived and functioned in the transplanted spaces with fibrin glue as well as with Matrigel for 28 days. Thus, this $in$ $vivo$ system using fibrin is expected to serve as a good model to further improve the transplanted cell/scaffold conditions for the stable and durable cell-based replacement of defective proteins in patients with LCAT deficiency.
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Yasuyuki Aoyagi,Yasushi Saito,Masayuki Kuroda,Sakiyo Asada,Hideaki Bujo,Shigeaki Tanaka,Shunichi Konno,Masami Tanio,Itsuko Ishii,Masayuki Aso 생화학분자생물학회 2011 Experimental and molecular medicine Vol.43 No.3
The development of clinically applicable scaffolds is important for the application of cell transplantation in various human diseases. The aims of this study are to evaluate fibrin glue in a novel protein replacement therapy using proliferative adipocytes and to develop a mouse model system to monitor the delivery of the transgene product into the blood and the fate of the transduced cells after transplantation. Proliferative adipocytes from mouse adipose tissue were transduced by a retroviral vector harboring the human lecithin-cholesterol acyltransferase (lcat) gene, and were subcutaneously transplanted into mice combined with fibrin glue. The lcat gene transduction efficiency and the subsequent secretion of the product in mouse adipocytes were enhanced using a protamine concentration of 500 μg/ml. Adipogenesis induction did not significantly affect the lcat gene-transduced cell survival after transplantation. Immunohistochemistry showed the ectopic enzyme production to persist for 28 days in the subcutaneously transplanted genetransduced adipocytes. The increased viability of transplanted cells with fibrin glue was accompanied with the decrease in apoptotic cell death. The immunodetectable serum LCAT levels in mice implanted with the fibrin glue were comparable with those observed in mice implanted with Matrigel, indicating that the transplanted lcat gene-transduced adipocytes survived and functioned in the transplanted spaces with fibrin glue as well as with Matrigel for 28 days. Thus,this in vivo system using fibrin is expected to serve as a good model to further improve the transplanted cell/scaffold conditions for the stable and durable cell-based replacement of defective proteins in patients with LCAT deficiency.
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