http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Maquat, Lynne E (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
-
The Dharma of Nonsense-Mediated mRNA Decay in Mammalian Cells
Popp, Maximilian Wei-Lin,Maquat, Lynne E. Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.1
Mammalian-cell messenger RNAs (mRNAs) are generated in the nucleus from precursor RNAs (pre-mRNAs, which often contain one or more introns) that are complexed with an array of incompletely inventoried proteins. During their biogenesis, pre-mRNAs and their derivative mRNAs are subject to extensive cis-modifications. These modifications promote the binding of distinct polypeptides that mediate a diverse array of functions needed for mRNA metabolism, including nuclear export, inspection by the nonsense-mediated mRNA decay (NMD) quality-control machinery, and synthesis of the encoded protein product. Ribonucleoprotein complex (RNP) remodeling through the loss and gain of protein constituents before and after pre-mRNA splicing, during mRNA export, and within the cytoplasm facilitates NMD, ensuring integrity of the transcriptome. Here we review the mRNP rearrangements that culminate in detection and elimination of faulty transcripts by mammalian-cell NMD.
-
Failsafe nonsense-mediated mRNA decay does not detectably target eIF4E-bound mRNA
Matsuda, Daiki,Hosoda, Nao,Kim, Yoon Ki,Maquat, Lynne E Nature Publishing Group 2007 Nature Structural and Molecular Biology Vol.14 No.10
Nonsense-mediated mRNA decay (NMD) generally eliminates messenger RNAs that prematurely terminate translation and occurs in all eukaryotes that have been studied, although with mechanistic variations. In mammals, NMD seems to be restricted to newly synthesized mRNA that is bound by the cap-binding heterodimer CBP80-CBP20 (CBP80/20) and typically has at least one exon junction complex (EJC) situated downstream of the nonsense codon and added post-splicing. However, mammalian NMD can also target spliced mRNA lacking an EJC downstream of the nonsense codon. Here we provide evidence that this additional pathway, known as failsafe NMD, likewise seems to be restricted to CBP80/20-bound mRNA and does not detectably target its subsequently remodeled product, eIF4E-bound mRNA. Our studies, including analyses of factor dependence, reveal important shared features of the two mammalian-cell NMD pathways as well as fundamental differences between NMD in mammals and Saccharomyces cerevisiae.
-
The Dharma of Nonsense-Mediated mRNA Decay in Mammalian Cells
Maximilian Wei-Lin Popp,Lynne E. Maquat 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.1
Mammalian-cell messenger RNAs (mRNAs) are generat-ed in the nucleus from precursor RNAs (pre-mRNAs, which often contain one or more introns) that are com-plexed with an array of incompletely inventoried proteins. During their biogenesis, pre-mRNAs and their derivative mRNAs are subject to extensive cis-modifications. These modifications promote the binding of distinct polypeptides that mediate a diverse array of functions needed for mRNA metabolism, including nuclear export, inspection by the nonsense-mediated mRNA decay (NMD) quality-control machinery, and synthesis of the encoded protein product. Ribonucleoprotein complex (RNP) remodeling through the loss and gain of protein constituents before and after pre-mRNA splicing, during mRNA export, and within the cytoplasm facilitates NMD, ensuring integrity of the transcriptome. Here we review the mRNP rearrangements that culminate in detection and elimination of faulty transcripts by mammalian-cell NMD.
-
Upf1 Phosphorylation Triggers Translational Repression during Nonsense-Mediated mRNA Decay
Isken, Olaf,Kim, Yoon Ki,Hosoda, Nao,Mayeur, Greg L.,Hershey, John W.B.,Maquat, Lynne E. Elsevier 2008 Cell Vol.133 No.2
<P><B>Summary</B></P><P>In mammalian cells, nonsense-mediated mRNA decay (NMD) generally requires that translation terminates sufficiently upstream of a post-splicing exon junction complex (EJC) during a pioneer round of translation. The subsequent binding of Upf1 to the EJC triggers Upf1 phosphorylation. We provide evidence that phospho-Upf1 functions after nonsense codon recognition during steps that involve the translation initiation factor eIF3 and mRNA decay factors. Phospho-Upf1 interacts directly with eIF3 and inhibits the eIF3-dependent conversion of 40S/Met-tRNA<SUB>i</SUB><SUP>Met</SUP>/mRNA to translationally competent 80S/Met-tRNA<SUB>i</SUB><SUP>Met</SUP>/mRNA initiation complexes to repress continued translation initiation. Consistent with phospho-Upf1 impairing eIF3 function, NMD fails to detectably target nonsense-containing transcripts that initiate translation independently of eIF3 from the CrPV IRES. There is growing evidence that translational repression is a key transition that precedes mRNA delivery to the degradation machinery. Our results uncover a critical step during NMD that converts a pioneer translation initiation complex to a translationally compromised mRNP.</P>
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
