RISS 학술연구정보서비스

검색
다국어 입력

http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.

변환된 중국어를 복사하여 사용하시면 됩니다.

예시)
  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
  • 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
닫기
    인기검색어 순위 펼치기

    RISS 인기검색어

      검색결과 좁혀 보기

      선택해제

      오늘 본 자료

      • 오늘 본 자료가 없습니다.
      더보기
      • 무료
      • 기관 내 무료
      • 유료
      • KCI등재

        Double-Face Activity of Resveratrol in Voluntary Runners: Assessment of DNA Damage by Comet Assay

        Barbara Tomasello,Salvatore Grasso,Giuseppe Malfa,Stefania Stella,Marco Favetta,Marcella Renis 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.5

        Voluntary runners are subjected to a massive increase in reactive oxygen/nitrogen species production, which can promote different oxidative stress–related diseases such as premature aging, neurodegenerative disorders, and cancer. The aims of this work were to evaluate the following in peripheral blood cells of voluntary runners: (i) DNA status; (ii)susceptibility to the in vitro insult induced by hydrogen peroxide (H2O2) as a breaking agent; (iii) capabilities of 3,5,40-trihydroxystilbene (RESV) in counteracting DNA damage. Twenty-five male voluntary runners were compared with 20sedentary men, as age-matched controls, and DNA status was evaluated with different versions of comet assay: alkaline,neutral, and Fpg enzyme–modified version to measure 8-OH-deoxyguanosine (8-oxo-dG) levels. The H2O2 and/or RESV treatments were performed directly on agarose-embedded cells (atypical comet assay). The results evidenced DNA damage and levels of 8-oxo-dG higher in runners than in sedentary control subjects. The runners’ DNA was more prone to the in vitro–induced oxidative insult (200 lM H2O2) than that of the control group. Resveratrol (100 lM), depending on the individual basal DNA status, was able to switch from antioxidant to pro-oxidant. Our results, on the one hand, validated the proposed in vitro experimental protocol in order to measure individual DNA status. On the other hand, our data point out the importance of monitoring the athletes’ redox status before subjecting them to dietary supplementation treatment.

      연관 검색어 추천

      이 검색어로 많이 본 자료

      활용도 높은 자료

      해외이동버튼