Gnidilatimonoein from Daphne mucronata Induces Differentiation and Apoptosis in Leukemia Cell Lines

Mahdavi, Majid,Yazdanparast, Razieh 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.2

Gnidilatimonoein is a new diterpene ester, recently isolated from the leaves of Daphne macronata with potent anti-tumoral and anti-metastastic activities (Yazdanparast et al., 2004). Promyeloblastic (KG1), promyelocytic (NB4) and promonocytic (U937) cells were cultured in the presence of various concentrations of the drug (0.5-3.0 ${\mu}$M) for 3 days. Herein, we report that gnidilatimonoein induces differentiation and apoptosis in KG1 , NB4 and U937 cells. The drug inhibited growth and proliferation of KG1 , NB4 and U937 cells with IC50 values of 1.5, 1.5 and 1.0 ${\mu}$M, respectively, after 72 h of treatment. Cell viability was also decreased by 18%, 20% and 23%, respectively, after 72 h treatment with the drug. NBT reducing assay revealed that the inhibition of proliferation is associated with differentiation especially toward monocytes-like morphology. Indeed, the drug at 0.5-15 ${\mu}$M induced differentiation by 5-50% in the cells. Acridine orange/ethidium bromide (AO/EtBr) double staining and DNA fragmentation assays revealed that apoptosis occurred after differentiation of the cells. Based on the present data, it seems that the new compound is a good candidate for further evaluation as an effective chemotherapeutic agent acting through induction of differentiation and apoptosis.

Gnidilatimonoein from Daphne mucronata Induces Differentiation and Apoptosis in Leukemia Cell Lines

Majid Mahdavi,Razieh Yazdanparast 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.2

Gnidilatimonoein is a new diterpene ester, recently isolated from the leaves of Daphne macronata with potent anti-tumoral and anti-metastastic activities (Yazdanparast et al., 2004). Promyeloblastic (KG1), promyelocytic (NB4) and promonocytic (U937) cells were cultured in the presence of various concentrations of the drug (0.5-3.0 µM) for 3 days. Herein, we report that gnidilatimonoein induces differentiation and apoptosis in KG1, NB4 and U937 cells. The drug inhibited growth and proliferation of KG1, NB4 and U937 cells with IC50 values of 1.5, 1.5 and 1.0 µM, respectively, after 72 h of treatment. Cell viability was also decreased by 18%, 20% and 23%, respectively, after 72 h treatment with the drug. NBT reducing assay revealed that the inhibition of proliferation is associated with differentiation especially toward monocytes-like morphology. Indeed, the drug at 0.5-1.5 µM induced differentiation by 5-50% in the cells. Acridine orange/ethidium bromide (AO/EtBr) double staining and DNA fragmentation assays revealed that apoptosis occurred after differentiation of the cells. Based on the present data, it seems that the new compound is a good candidate for further evaluation as an effective chemotherapeutic agent acting through induction of differentiation and apoptosis.

Oxidative Stress-Induced Apoptosis in Chronic Myelogenous Leukemia K562 Cells by an Active Compound from the Dithio-Carbamate Family

Khoshtabiat, Laya,Mahdavi, Majid,Dehghan, Gholamreza,Rashidi, Mohammad Reza Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.9

Previous studies suggested that dithio-carbamates are potent apoptosis and anti-apoptosis inducing agents in various cancer cells. Here, the anti-proliferative and apoptosis inducing effects of a new derivative (2-NDC) from the dithio-carbamate family was examined in human leukemia K562 cells. We use thiazolyl blue tetrazolium bromide (MTT) to measure viability and cell growth inhibition. The 2-NDC showed effects on viability in a dose and time-dependent manner, inhibiting proliferation at concentrations of $10-30{\mu}M$ after 24-48 hours of treatment and increasing values after 72 hours at $40-120{\mu}M$. The cytotoxic effect of the compound was calculated with an $IC_{50}$ of $30{\mu}M$ after 24-hour. Apoptosis induction was confirmed by acridine orange-ethidium bromide (AO/EtBr) staining, DNA fragmentation assay, flow cytometric assessment and also caspase-3 activation assay. Furthermore, enzymes level such as superoxide dismutase (SOD) and catalase (CAT) involved in oxidative stress were evaluated. The results of this study demonstrated insignificant increase of intracellular ROS levels for 24 hours and reduction after 48-72 hours. In addition to reduction of intracellular thiol, caspase-3 like activity was also decreased in a time-dependent manner in cells treated with 2-NDC. Thus 2-NDC can be considered as a good candidate for further pharmaceutical evaluations.

Anti-proliferative and Apoptotic Effects of the Derivatives from 4- aryl-4H-chromene Family on Human Leukemia K562 Cells

Hassan Aryapour,Majid Mahdavi,Seyed Reza Mohebbi,Mohammad Reza Zali,Alireza Foroumadi 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.9

Previous studies suggest that 4-aryl-4H-chromenes are potent apoptosis-inducing agents in various cancer cell lines. In this study, anti-proliferative and apoptotic effects of the derivatives from 4-aryl-4H-chromene family were investigated in the human leukemia K562 cells using [3-(4,5)-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide (MTT) growth inhibition assay. 3-NC was more active among these compounds with IC50 of 65 nM and was selected for further studies. Apoptosis, as the mechanism of cell death, was investigated morphologically by Hoechst 33258 staining, cell surface expression assay of phosphatidylserine by Annexin V/PI technique,caspase-3 activation assay, as well as the formation of DNA ladder. The K562 cells underwent apoptosis upon a single dose (at IC50 value) of the compound, and also increased caspase-3 activity by more than 2.3-fold, following a 72 h treatment. Caspase-9 was also activated which could be detected 48 hours post-treatment. Furthermore, Western blot analysis revealed that the treatment with the compound down-regulated the expression of certain IAP protein, including survivin. These data further suggest that these derivatives from 4-aryl-4H-chromene may provide a novel therapeutic approach for the treatment of leukemia.

Chemical modification of polystyrene foam using functionalized chitosan with dithiocarbamate as an adsorbent for mercury removal from aqueous solutions

Babak Porkar,Pourya Alipour Atmianlu,Mahyar Mahdavi,Majid Baghdadi,Hamidreza Farimaniraad,Mohammad Ali Abdoli 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.4

One of the major environmental issues today is waste pollution, particularly non-biodegradable wastes such as polystyrene waste. Furthermore, heavy metal contamination is a major environmental threat. Mercury is one of the most hazardous and poisonous contaminants, and its usage in various industrial processes has resulted in contaminated effluents being released into surface runoff and groundwater. Because of the beneficial physical properties of polystyrene foam, this non-biodegradable waste was used in this study as a suitable medium for chemical modification. The polystyrene foam was first modified using crosslinked chitosan, and then it was reacted with carbon disulfide to improve its performance for the removal of Hg2+. The prepared composite was used for the removal of mercury ions from contaminated water. The adsorbent’s physical, chemical, and morphological properties were determined using energy-dispersive spectroscopy (EDS), Fourier-transform infrared spectroscopy (FT-IR), Field emission scanning electron microscopy (FE-SEM), and Brauer-Emmett-Teller (BET) analyses. Specific surface area, porosity, and average pore diameter were determined to be 314.8 m2/g, 0.345 cm2/g, and 1.96 nm, respectively. Experiments were designed to investigate the effects of pH, contact time, and contaminant concentration by the Box-Behnken response surface methodology. The maximum removal percentage of 79.85% was achieved for the initial mercury concentration of 50 mg/L at pH 4. Moreover, the adsorption was observed to follow the Dubinin-Radushkevich isotherm. Studies on adsorbent recovery also showed that the adsorbent can be recovered and reused for at least three cycles.

The Effect of Carum Copticum Extract on Acetylcholine Induced Contraction in Isolated Rat’s Ileum

Seyed Hassan Hejazian-Y,Mohammad Hossein Dashti-R,Seyed Majid Mahdavi,Masood Anvar Qureshi 사단법인약침학회 2009 Journal of Acupuncture & Meridian Studies Vol.2 No.1

Aims: There are many biological investigations for determining an effective cure for the dysfunction of gastrointestinal tracts, using herbal medicine. It has been reported that Carum Copticum is a bactericidal agent and possesses anticholinergic, antihistaminic and β-adrenergic stimulatory effects in some tissues. However, these effects of Carum Copticum on mechanical activities of isolated intestine are not clearly identified yet. The present study has been designed to find out the specific effects of Carum Copticum on mechanical activity of isolated rat’s ileum. Materials and Methods: In this study rat’s ileum contraction was recorded through an isolated tissue chamber in an organ bath by using isotonic transducer and oscillographic device. The effect of Carum Copticum extract on acetylcholine induced contraction in isolated rat’s ileum was evaluated. Results: Our findings showed that 1% aqueous extract of Carum Copticum reduces the basal contractile activity of rat’s ileum. The extract also reduced acetylcholine induced contraction to 40% of its maximum response. The inhibitory action of Carum Copticum extract on acetylcholine induced contraction was similar but slower than that of atropine sulfate. Conclusion: The results of this study showed an inhibitory effect of Carum Copticum extract on acetylcholine induced contraction in rat’s ileum. Aims: There are many biological investigations for determining an effective cure for the dysfunction of gastrointestinal tracts, using herbal medicine. It has been reported that Carum Copticum is a bactericidal agent and possesses anticholinergic, antihistaminic and β-adrenergic stimulatory effects in some tissues. However, these effects of Carum Copticum on mechanical activities of isolated intestine are not clearly identified yet. The present study has been designed to find out the specific effects of Carum Copticum on mechanical activity of isolated rat’s ileum. Materials and Methods: In this study rat’s ileum contraction was recorded through an isolated tissue chamber in an organ bath by using isotonic transducer and oscillographic device. The effect of Carum Copticum extract on acetylcholine induced contraction in isolated rat’s ileum was evaluated. Results: Our findings showed that 1% aqueous extract of Carum Copticum reduces the basal contractile activity of rat’s ileum. The extract also reduced acetylcholine induced contraction to 40% of its maximum response. The inhibitory action of Carum Copticum extract on acetylcholine induced contraction was similar but slower than that of atropine sulfate. Conclusion: The results of this study showed an inhibitory effect of Carum Copticum extract on acetylcholine induced contraction in rat’s ileum.

Helicobacter pylori vacA d1 Genotype Predicts Risk of Gastric Adenocarcinoma and Peptic Ulcers in Northwestern Iran

Basiri, Zeinab,Safaralizadeh, Reza,Bonyadi, Morteza Jabbarpour,Somi, Mohammad Hossein,Mahdavi, Majid,Latifi-Navid, Saeid Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

Background: There is a close relationship between Helicobacter pylori (H pylori)-specific factors and different gastroduodenal diseases. The present study aimed to investigate the prevalence of vacA d1, d2 genotypes in the H pylori isolates from patients with gastric adenocarcinoma, peptic ulcer disease (PUD) and gastritis in East Azerbaijan region, where the incidence of gastric cancer (GC) is high. Strains isolated from this area are likely to be of European ancestry. Materials and Methods: In this study, genotyping of the vacA d region of 115 isolates obtained from patients with different gastrodoudenal diseases was accomplished by PCR methods. In addition to PCR amplification of H pylori 16S rDNA, rapid urease tests or histological examination were used to confirm the presence of H pylori in biopsy specimens. Data were collected and analyzed using SPSS version 19. Results: Of the total of 83 H pylori isolates, 36 (43.4%) contained the d1 allele and 47 (56.6%) were subtype d2. The results of the multiple linear/logistic regression analysis showed high correlation between allele d1 and gastric adenocarcinoma or PUD. Conclusions: This study suggests that the H pylori vacA d1 genotype helps predict risk for gastric adenocarcinoma and PUD in East Azerbaijan, Iran.