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- 저자 : Mahto, Shruti (검색결과 18 건)
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Cytotoxic Potentials of Tellurium Nanowires in BALB/3T3 Fibroblast Cells
Mahto, Sanjeev Kumar,Vinod, T.P.,Kim, Jin-Kwon,Rhee, Seog-Woo Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.9
We have investigated the cytotoxic potential of tellurium (Te) nanowires in BALB/3T3 fibroblast cells. Te nanowires were synthesized through an aqueous phase surfactant assisted method. Toxicological experiments, such as analysis of morphological changes, MTT assay, DAPI staining, and estimation of intracellular reactive oxygen species, were carried out to reveal the cytotoxic effects of Te nanowires. Te nanowires were found to be cytotoxic at all concentrations tested, in a dose-dependent manner. The UV/Vis spectra of Te nanowires suspended in a culture medium showed drastic changes and disappearance of two broad absorption peaks. The physicochemical properties such as, surface charge, size, and shape of Te nanowires were found to be altered during exposure of cells, due to the instability and agglomeration of nanowires in the culture medium. These results suggest that the chemical components of the DMEM medium significantly affect the stability of Te nanowires. In addition, TEM images revealed that necrosis was the basic pattern of cell death, which might stem from the formation of toxic moieties of tellurium, released from nanowire structures, in the bioenvironment. These observations thus suggest that Te nanomaterials may pose potential risks to environmental and human health.
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Mahto, Uttam K.,Roy, Sumit K.,Chaudhuri, S.,Prasad, K. Techno-Press 2016 Advances in materials research Vol.5 No.3
In this work effect of high energy milling on the structural and electrical properties of $Ba(Fe_{1/2}Ta_{1/2})O_3$ (BFT) ceramic synthesized using standard solid-state reaction method were investigated. X-ray diffraction studies indicated that the unit cell structure for all the samples to be hexagonal (space group: P3m1). FTIR spectra also confirmed the formation of BFT without any new phase. The milled (10 h) BFT ceramic showed the formation of small grain sizes (<$2{\mu}m$) which is beneficial for dielectric applications in high density integrated devices. Besides, the milled (10 h) BFT ceramic sample exhibited superior dielectric properties (enhancement in ${\varepsilon}^{\prime}-value$ and reduction in $tg{\delta}-value$) compared to un-milled one. Impedance analysis indicated the negative temperature coefficient of resistance (NTCR) character. The correlated barrier hopping model (jump relaxation type) is found to successfully explain the mechanism of charge transport in present ceramic samples.
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Characterizing Cell Death Events Using a Microfluidics-based Method
Sanjeev Kumar Mahto,윤태현,이석우 한국바이오칩학회 2008 BioChip Journal Vol.2 No.4
Cell death is the ultimate endpoint of the cellular life cycle, and the ability to distinguish different types of cell death (e.g., apoptosis and necrosis) has been a subject of intensive research. This paper demonstrates the suitability and efficiency of a microfluidicsbased method for characterization of cell death events. A multicompartmented microfluidic device was used to examine the types of cell death induced by cadmium ions at various concentrations. Annexin V-FITC in combination with propidium iodide was used to distinguish between viable, poptotic, and necrotic cells. The microfluidics-based method facilitates the performance of analysis of cell death on the basis of membrane alteration as well as morphological discrimination. In addition, this method shows high sensitivity and specificity, as evidenced by the quantification of the ratio of apoptotic and necrotic cells in the total population of cells.
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Sanjeev Kumar Mahto,이석우 한국바이오칩학회 2012 BioChip Journal Vol.6 No.1
This work describes the development of a multi-inlet microfluidic device for in situ detection of multiple cytotoxicity endpoints. The device consists of an upstream microfluidic multi-inlet module (μMIM) and a downstream microfluidic cell culture channel (μCCC). The integration of a device with syringe pumps via the inlets of μMIM mainly enables the performance of multiplex cytotoxicity assays targeted on multiple endpoints. The device in the study was used for the long-term culturing of BALB/3T3 fibroblast cells. In addition, cadmium ion was used as a toxicant to induce the toxicity in the cultured cells. The cytotoxicity endpoints, such as the in situ detection of reactive oxygen species (ROS), nuclear structures and cell morphology, were examined and correlated simultaneously for every single cell after they were exposed to cadmium. The data indicated that the percentage of cells that had produced ROS and shown nuclear staining were slightly higher than the cells that had produced ROS only, but this percentage was significantly higher than the cells that had shown only nuclear staining. The results revealed that the obtained correlated data from simultaneous observations of multiplex cytotoxicity assays were more efficient in providing the mechanistic explanation for Cd-induced toxicity than the individual data that were derived after examining a single endpoint. In addition, the results suggested that the intracellular formation of ROS induced by cadmium might have been followed by the alterations in cell and nuclear morphology and loss of membrane integrity. The device allows for the real-time monitoring of cells and their behavior against a toxicant and facilitates the rapid performance of cytotoxicity testing. Furthermore, we anticipate that the presented device could be very effective in advancing the understanding of the precise mechanism of cytotoxicity by improving the sensitivity and accuracy in identifying endpoints.
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Cytotoxic Potentials of Tellurium Nanowires in BALB/3T3 Fibroblast Cells
Sanjeev Kumar Mahto,Jinkwon Kim,T. P. Vinod,Seog Woo Rhee 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.9
We have investigated the cytotoxic potential of tellurium (Te) nanowires in BALB/3T3 fibroblast cells. Te nanowires were synthesized through an aqueous phase surfactant assisted method. Toxicological experiments,such as analysis of morphological changes, MTT assay, DAPI staining, and estimation of intracellular reactive oxygen species, were carried out to reveal the cytotoxic effects of Te nanowires. Te nanowires were found to be cytotoxic at all concentrations tested, in a dose-dependent manner. The UV/Vis spectra of Te nanowires suspended in a culture medium showed drastic changes and disappearance of two broad absorption peaks. The physicochemical properties such as, surface charge, size, and shape of Te nanowires were found to be altered during exposure of cells, due to the instability and agglomeration of nanowires in the culture medium. These results suggest that the chemical components of the DMEM medium significantly affect the stability of Te nanowires. In addition, TEM images revealed that necrosis was the basic pattern of cell death, which might stem from the formation of toxic moieties of tellurium, released from nanowire structures, in the bioenvironment. These observations thus suggest that Te nanomaterials may pose potential risks to environmental and human health.
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Sanjeev Kumar Mahto,송휴섭,이석우 한국바이오칩학회 2011 BioChip Journal Vol.5 No.4
In this work, the formation of functional synapses between compartmentalized cortical neurons cultured inside three-compartment microfluidic devices in a controlled fashion is described. The proposed device can direct axons in an isolated compartment and, thus, facilitates isolated axons forming functional synapses with dendrites of other neurons in an isolated microenvironment. This microfluidic approach allows continuous real-time monitoring of neuronal processes and fluorescently tagged biomolecules involved in synapse formation, and provides an easy, simple, cost effective, and efficient method to develop and manipulate synapses in an isolated microenvironment without using surface patterning techniques or electrical stimulation. The results presented here suggest that this microfluidic approach could be used as an alternative method for the formation of functional synapses and their exhaustive examinations.
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Raju Prasad Mahto,Sharath Anishetty,Arnab Sarkar,Omkar Mypati,Surjya Kanta Pal,Jyotsna Dutta Majumdar 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.3
The use of aluminum in conjunction of steel can reduce the weight of structures but dissimilar materials welded structureresults in the formation of intermetallic compounds and inhomogeneous distribution of grains. Since aluminum is moreactive than the steel, the structures made from such dissimilar materials can be affected from corrosion medium which needsto be investigated. In the present work, friction stir welding has been used to join AA6061-T6 and AISI304 in lap configuration,each having a thickness of 1 mm under varied process parameters. The detailed investigations have been made whichincludes understanding the effect of process variables on microstructures, intermetallic compounds and their phases, andthereby on corrosion of the aluminum-steel welded joint. SEM with integrated EBSD detector and XRD analyses have beencarried out to characterize the weld interface that revealed the evolution of grain boundaries and existence of phases suchas Fe2Al5and AlCrFe2. The grain size of the weld zone has been found to be decreasing with increase in weld speed andplunge depth. The temperature profiles have shown a faster rate of heating and cooling with increase in welding speed andplunge depth which led to the refinement of microstructure. The evolution precipitates mainly comprised of Al, Mg and Sias the major elements. The corrosion rate was found to be increasing with decrease in grain size. Samples were corroded bypitting corrosion, inter-granular corrosion, and environmental corrosion. Severity of pits have been found to be non-uniformin the along weld cross-section.
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