Curcumin induces brown fat-like phenotype in 3T3-L1 and primary white adipocytes

Lone, J.,Choi, J.H.,Kim, S.W.,Yun, J.W. Butterworths ; Elsevier Science Ltd 2016 The Journal of nutritional biochemistry Vol.27 No.-

<P>Recent advances have been made in the understanding of pharmacological and dietary agents that contribute to browning of white adipose tissue in order to combat obesity by promoting energy expenditure. Here, we show that curcumin induces browning of 3T3-L1 and primary white adipocytes via enhanced expression of brown fat-specific genes. Curcumin-induced browning in white adipocytes was investigated by determining expression levels of brown adipocyte-specific genes/proteins by realtime reverse transcriptase polymerase chain reaction, immunoblot analysis and immunocytochemical staining. Curcumin increased mitochondrial biogenesis, as evidenced by transmission electronic microscopic detection and enhanced expression of proteins involved in fat oxidation. Cucurmin also increased protein levels of hormone-sensitive lipase and p-acyl-CoA carboxylase, suggesting its possible role in augmentation of lipolysis and suppression of lipogenesis. Increased expression of UCP1 and other brown adipocyte-specific markers was possibly mediated by curcumin-induced activation of AMP-activated protein kinase (AMPK) based on the fact that inhibition of AMPK by dorsomorphin abolished expression of PRDM16, UCP1 and peroxisome proliferator-activated receptor gamma co-activator 1-alpha while the activator 5-Aminoimidazole-4-carboxamide ribonucleotide elevated expression of these brown marker proteins. Our findings suggest that curcumin plays a dual modulatory role in inhibition of adipogenesis as well as induction of the brown fat-like phenotype and thus may have potential therapeutic implications for treatment of obesity. (C) 2015 Elsevier Inc. All rights reserved.</P>

Preparation of urushiol-containing poly(methyl methacrylate) copolymers for antibacterial and antifouling coatings

Lone, N.,Cheong, I. W.,Cho, M.,Hong, Y. K.,Choi, Y. S.,Perumal, S.,Oh, B. T.,Joo, J. Springer Science + Business Media 2017 Journal of coatings technology and research Vol.14 No.3

<P>Synthesis of an eco-friendly and efficient antibacterial and antifouling coatings is presented by exploiting urushiol, a natural varnishing material. Since urushiol has inherent outstanding surface-protecting and antimicrobial properties, a series of poly (methyl methacrylate)-urushiol polymer compositions were prepared and fabricated into films. The prepared films were subjected to antimicrobial and antifouling studies. The polymer systems were characterized by various physico-spectroscopic techniques such as H-1 NMR, Fourier transform infrared spectroscopy, differential scanning calorimetry, atomic force microscopy, and thermal gravimetric analysis. The confocal laser scanning micrographs, obtained for Pseudomonas aeruginosa biofilm formation, demonstrated an excellent antimicrobial response of the urushiol-incorporated polymers against this bacterial strain. We also demonstrated an inhibitory attachment effect against Navicula incerta, a fouling microalgal strain.</P>

Microfluidic synthesis of Janus particles by UV-directed phase separation

Lone, Saifullah,Kim, Sung Hoon,Nam, Seong Won,Park, Sungsu,Joo, Jin,Cheong, In Woo Royal Society of Chemistry 2011 Chemical communications Vol.47 No.9

<P>A synthetic methodology based on microfluidics has been developed to fabricate monodisperse polymer Janus particles by UV-directed phase separation.</P> <P>Graphic Abstract</P><P>A synthetic methodology based on microfluidics has been developed to fabricate monodisperse polymer Janus particles by UV-directed phase separation. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0cc04517a'> </P>

Photoresponsive Phase Separation of a Poly(NIPAAm-<i>co</i>-SPO-<i>co</i>-fluorophore) Random Copolymer in W/O Droplet

Lone, Saifullah,Ahn, Jeong In,Kim, Mi Ri,Lee, Hyang Moo,Kim, Sung Hoon,Lodge, Timothy P.,Cheong, In Woo American Chemical Society 2014 Langmuir Vol.30 No.31

<P>The photoresponsive phase separation of a poly(<I>N-</I>isopropylacrylamide-<I>co-</I>spironaphthoxazine methacryloyl-<I>co-</I>allyl<I>-</I>2<I>-</I>(2,6<I>-</I>bis((<I>E</I>)<I>-</I>4<I>-</I>(diphenylamino)styryl)<I>-</I>4<I>H-</I>pyran<I>-</I>4<I>-</I>ylidene)<I>-</I>2<I>-</I>cyanoacetate) random copolymer, i.e., poly(NIPAAm-<I>co</I>-SPO-<I>co</I>-fluorophore), in water-in-oil (W/O) droplets is described. The photoresponsive aqueous droplets were generated in the coflow regime of a simple tubular microfluidic device. The phase separation of the copolymer in the W/O droplets was induced by UV light at 365 nm and was affected significantly by the presence of 2,2-diethoxyacetophenone (DEAP) and sorbitan monooleate (Span 80). When the droplets were subjected to UV irradiation for more than 2 min, the phase-separated copolymer was transferred completely from the aqueous droplet to the continuous phase of hexadecane. The phase separation arises from the photoisomerization shifting the spiro to the merocyanine form of the SPO pendant group in the copolymer, which in turn reduces the hydrophilicity of the copolymer via attractive hydrogen-bonding interactions between the merocyanine group and hydrophobic additives, i.e., Span 80, DEAP, and some stable fragments derived from the photocleavage of DEAP under UV irradiation. These interactions cause the copolymer to associate with the additives and then accelerate the phase separation of the copolymer and subsequent phase transfer of copolymer aggregates. The separate effects of DEAP and Span 80 were also investigated by UV spectrophotometric analysis of the rate coefficient of the reverse transformation (merocyanine to spiro) of the photochromic monomer. We propose a mechanism of phase separation of the copolymer in the W/O droplet based on the NMR and GC-MASS analyses of DEAP.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/langd5/2014/langd5.2014.30.issue-31/la5022005/production/images/medium/la-2014-022005_0012.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/la5022005'>ACS Electronic Supporting Info</A></P>

Facile and highly efficient microencapsulation of a phase change material using tubular microfluidics

Lone, S.,Lee, H.M.,Kim, G.M.,Koh, W.G.,Cheong, I.W. Elsevier 2013 Colloids and surfaces. A, Physicochemical and engi Vol.422 No.-

This paper presents a microfluidic approach towards the fabrication of highly monodisperse polyurea microcapsules, 35-500μm in size, containing n-octadecane (phase change material, PCM). The synthesis consisted of the following two steps: (i) emulsification of n-octadecane, isophorone diisocyanate (IPDI) and dibutyltin dilaurate (DBTDL) in an aqueous mixture of tetraethylenepentamine (TEPA), poly (vinyl alcohol) and sodium dodecyl sulfate (SDS)and (ii) in situ polycondensation between TEPA and IPDI along and outside the tube length. The resulting PCMπolyurea microcapsules underwent a rapid and repeated liquid/solid phase transformation at different temperatures. The average sizes and morphology of the microcapsules were controlled by tuning the flow rate of either the continuous or discontinuous phase. The morphology, polycondensation and phase change behavior of the microcapsules were investigated by optical microscopy equipped with a thermostat plate, scanning electron microscopy, Fourier transform infrared spectroscopy and differential scanning calorimetry. The effect of the hydrophobic Fe<SUB>3</SUB>O<SUB>4</SUB> nanoparticles (NPs) on the crystallization behavior was also examined in cases of microcapsules with or without NPs.

Honokiol exerts dual effects on browning and apoptosis of adipocytes

Lone, Jameel,Yun, Jong Won INSTITUTE OF PHARMCOLOGY POLISH ACADEMY OF SCIENCE 2017 PHARMACOLOGICAL REPORTS Vol.69 No.6

<P>Conclusion: Our findings suggest that honokiol exhibits a modulatory role in adipocytes via induction of browning and apoptosis in white adipocytes, promotion of catabolic lipid metabolism, as well as activation and inhibition of apoptosis in HIB1B brown adipocytes, thereby exhibiting therapeutic potential against obesity. (c) 2017 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Sp. z o.o. All rights reserved.</P>

Facile preparation of highly monodisperse poly(NIPAAm)–AuNP composite hollow microcapsules by simple tubular microfluidics

Lone, Saifullah,Sampatrao Ghodake, Gajanan,Sung Lee, Dae,Cheong, In Woo The Royal Society of Chemistry 2013 New journal of chemistry Vol.37 No.4

<P>We present an easy tubular microfluidic fabrication of highly monodisperse poly(NIPAAm)–AuNP composite hollow microcapsules with pickering effect assisted impregnation of AuNPs into the walls of microcapsules <I>via</I> UV-induced interfacial free-radical polymerization (IFRP). We maintained an excellent control over the monodispersity and capsule size distribution and the particles showed good thermosensitivity. The microfluidic device is simple and flexible in making; therefore, it permits a tunable UV curing chamber to control the polymerization time. Additionally, we present insight into the correlation between the polymerization time and the structural transformations (breathing, buckling and creasing) in the polymeric microcapsules. The structural transformations are independent of AuNPs embedded in the polymeric shell.</P> <P>Graphic Abstract</P><P>Tubular microfluidic fabrication of highly monodisperse poly(NIPAAm)–AuNP composite hollow microcapsules with pickering effect assisted impregnation of AuNPs into the walls of microcapsules <I>via</I> UV-induced interfacial free-radical polymerization (IFRP). <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3nj41133h'> </P>

Gelatin-chitosan hydrogel particles for efficient removal of Hg(ii) from wastewater

Lone, Saifullah,Yoon, Dong Ho,Lee, Hyangmoo,Cheong, In Woo The Royal Society of Chemistry 2019 Environmental science Vol.5 No.1

<P>Unregulated discharge of nonbiodegradable heavy metals (HMs) from mining operations, tanneries, and metal plating is posing an impending global water pollution concern. Early detection, accurate determination, and practical removal of HMs from industrial water are vital for a clean ecosystem and critical for the survival of imperiled aquatic life. Herein we report a robust, green, and cost-effective approach for the removal of heavy metal ions such as Pb(ii), Hg(ii), Cd(ii), and Cr(iii) from wastewater using a synthetic bioadsorbent made of gelatin-chitosan (GC) hydrogel particles, operated at neutral pH and laboratory temperature. The chemical and structural morphology of the synthesized hydrogel was confirmed by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM), respectively. HM ion adsorption was first tracked by the coffee ring effect (CRE) and subsequently characterized by inductively coupled plasma mass spectrometry (ICP-MS). As compared to Pb(ii), Cd(ii), and Cr(iii) metal ions, the hydrogel particles displayed a quite notable binding affinity for Hg(ii). Also, the hydrogel particles were examined for selective adsorption of HMs. As such, this strategy is robust and cheap, and could efficiently remove Hg(ii) metal ions from wastewater.</P>

Plasma Protein Profile of Neonatal Buffalo Calves in Relation to the Protein Profile of Colostrum/Milk during First Week Following Parturition

Lone, Abdul Gani,Singh, Charanbir,Singha, S.P.S. Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.3

An investigation was made into the protein profile of colostrum/milk of ten Murrah buffaloes and of their ten buffalo calves during their first week of neonatal life to study the materno-neonatal transfer of immunoglobulins (Ig). Calves were pail fed 3.5 liter of colostrum and/or milk per calf/day exclusively from their dam. First blood sample from newborn calves was collected before colostrum feeding on the day of birth (day zero) and the sampling continued daily for seven days after colostrum/milk feeding. Colostrum/milk Ig and IgG values were $4.82{\pm}2.60$, $2.19{\pm}1.90$, $1.12{\pm}0.82$, $0.69{\pm}0.44$, $0.59{\pm}0.31$, $0.47{\pm}0.20$, $0.40{\pm}0.22$, $0.40{\pm}0.25$ and $3.58{\pm}1.90$, $1.08{\pm}0.92$, $0.52{\pm}0.40$, $0.31{\pm}0.20$, $0.27{\pm}0.14$, $0.22{\pm}0.08$, $0.18{\pm}0.09$, $0.14{\pm}0.08$ respectively during 0-7 days post partum. The concentration of total colostrum/milk proteins, Ig, IgG and albumin were highest within 12 h post-partum. Thereafter, the concentrations followed a declining trend which may be attributed to the reduced transfer of proteins from the maternal blood, declining synthesis by the mammary glands and/or depletion of stored proteins. The concentrations of plasma Ig and IgG before colostrum feeding on day zero were $0.42{\pm}0.09$ and $0.08{\pm}0.03$ respectively. The levels of plasma Ig were $1.90{\pm}0.37$, $1.80{\pm}0.31$, $1.80{\pm}0.26$, $1.81{\pm}0.28$, $1.78{\pm}0.31$, $1.79{\pm}0.21$, $1.80{\pm}0.32$ and of IgG were $1.57{\pm}0.41$, $1.30{\pm}0.29$, $1.31{\pm}0.21$, $1.27{\pm}0.18$, $1.23{\pm}0.21$, $1.23{\pm}0.16$, $1.26{\pm}0.21$ on days 1-7 after birth after colostrum/milk feeding. The concentrations of total plasma proteins, Ig, IgG were lowest before colostrum feeding and increased significantly (p<0.05) after colostrum feeding in buffalo neonates. The results suggest that the highest amounts of colostral Ig and IgG were available on the day of parturition and thus the calves should receive colostrum as early after birth as possible. Colostrum Ig and IgG concentrations were not correlated to plasma Ig and IgG concentrations in the post-suckle buffalo calves and therefore, colostrum Ig and IgG concentrations were probably not the principle determinants of calf post-suckle plasma Ig and IgG concentrations.

Nobiletin Induces Brown Adipocyte-like Phenotype and Ameliorates Adipogenic Stress in 3T3L-1 Adipocytes

Jameel LONE,Jong Won YUN 한국생물공학회 2017 한국생물공학회 학술대회 Vol.2017 No.10