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Mattarucchi, Elia,Marsoni, Milena,Binelli, Giorgio,Passi, Alberto,Lo Curto, Francesco,Pasquali, Francesco,Porta, Giovanni Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.5
Single nucleotide polymorphisms (SNPs) are becoming the most common type of markers used in genetic analysis. In the present report a SNP has been chosen to test the applicability of Real Time PCR to discriminate and quantify SNPs alleles on DNA pools. Amplification Refractory Mutation System (ARMS) and Mismatch Amplification Mutation Assay (MAMA) has been applied. Each assay has been pre-validated testing specificity and performances (linearity, PCR efficiency, interference limit, limit of detection, limit of quantification, precision and accuracy). Both the approaches achieve a precise and accurate estimation of the allele frequencies on pooled DNA samples in the range from 5% to 95% and don't require standard curves or calibrators. The lowest measurement that could be significantly distinguished from the background noise has been determined around the 1% for both the approaches, allowing to extend the range of quantifications from 1% to 99%. Furthermore applicability of Real Time PCR assays for general diagnostic purposes is discussed.
STELLAR VARIABILITY OF THE EXOPLANET HOSTING STAR HD 63454
Kane, Stephen R.,Dragomir, Diana,Ciardi, David R.,Lee, Jae-Woo,Lo Curto, Gaspare,Lovis, Christophe,Naef, Dominique,Mahadevan, Suvrath,Pilyavsky, Genady,Udry, Stephane,Wang, Xuesong,Wright, Jason IOP Publishing 2011 The Astrophysical journal Vol.737 No.2
<P>Of the hundreds of exoplanets discovered using the radial velocity ( RV) technique, many are orbiting close to their host stars with periods less than 10 days. One of these, HD 63454, is a young active K dwarf which hosts a Jovian planet in a 2.82 day period orbit. The planet has a 14% transit probability and a predicted transit depth of 1.2%. Here we provide a re-analysis of the RV data to produce an accurate transit ephemeris. We further analyze 8 nights of time series data to search for stellar activity both intrinsic to the star and induced by possible interactions of the exoplanet with the stellar magnetospheres. We establish the photometric stability of the star at the 3 mmag level despite strong Ca II emission in the spectrum. Finally, we rule out photometric signatures of both star-planet magnetosphere interactions and planetary transit signatures. From this we are able to place constraints on both the orbital and physical properties of the planet.</P>