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Characterization of the Structure and DNA Complexity of Mung Bean Mitochondrial Nu-cleoids
Yih-Shan Lo,Lin-June Hsiao,Ning Cheng,Alexandra Litvinchuk,Hwa Dai 한국분자세포생물학회 2011 Molecules and cells Vol.31 No.3
Electron microscopic images of mitochondrial nucleoids isolated from mung bean seedlings revealed a relatively homogeneous population of particles, each consisting of a chromatin-like structure associated with a membrane com-ponent. Association of F-actin with mitochondrial nucleoids was also observed. The mitochondrial nucleoid structure identified in situ showed heterogeneous genomic organization. After pulsed-field gel electrophoresis (PFGE), a large proportion of the mitochondrial nucleoid DNA remained in the well, whereas the rest migrated as a 50-200 kb smear zone. This PFGE migration pattern was not af-fected by high salt, topoisomerase I or latrunculin B treatments; however, the mobility of a fraction of the fast-moving DNA decreased conspicuously following an in-gel ethidium-enhanced UV-irradiation treatment, suggesting that molecules with intricately compact structures were present in the 50-200 kb region. Approximately 70% of the mitochondrial nucleoid DNA molecules examined via electron microscopy were open circles, supercoils, complex forms, and linear molecules with interspersed sigma-shaped structures and/or loops. Increased sensitivity of mtDNA to DNase I was found after mitochondrial nucleoids were pretreated with high salt. This result indicates that some loosely bound or peripheral DNA binding proteins protected the mtDNA from DNase I degradation.
Information Technology In The Management Of Students' Activities While Studying
Zozuliak-Sluchyk, Roksoliana,Tytova, Nataliia,Kozliuk, Oleksandr,Salata, Halyna,Ridei, Nataliia,Yashnyk, Svitlana,Litvinchuk, Svitlana International Journal of Computer ScienceNetwork S 2021 International journal of computer science and netw Vol.21 No.2
The method of analysis and research is applied in the work methods of managing student activities. Effective forms and methods of student quality management are determined. The model of management of educational process of students is offered. The model is to activate the student's potential, while maintaining the classical educational paradigm. The analysis of features of management of activity of students in modern model of education is carried out.