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Transcriptome sequencing and analysis of sweet osmanthus (Osmanthus fragrans Lour.)
Hong Na Mu,Liang Gui Wang,Huo Gen Li,Xiu Lian Yang,Tao Ze Sun,Chen Xu 한국유전학회 2014 Genes & Genomics Vol.36 No.6
Osmanthus fragrans is a woody, evergreenspecies of shrubs and small trees that is extensively plantedin sub-tropical and temperate climates as an ornamentalplant in gardens and for its health benefits. The flower colorranges from ivory to orange to pink among different varietiesand even color difference during the whole blossom inthe sweet osmanthus. Sweet osmanthus is widely cultivatedthroughout China and other countries due to its prominentfragrance, colorful flowers, and medicinal properties. However, the scanty genomic resources in the Olea familyhave greatly hindered further exploration of its geneticmechanism on these economically important traits. In thisstudy, transcriptome sequencing of O. fragrans was performedusing the Illumina HighSeqTM2000 sequencingplatform. Next generation sequencing (NGS) of the transcriptomeof O. fragrans produced 31.7G of clean bases(211,266,818 clean reads) that were assembled into256,774 transcripts and 117,595 unigenes. Of them, 197and 237 candidate genes involved in fragrance and pigmentbiosynthesis respectively were identified based on functionannotation. Meanwhile, 1 unnamed protein and 468 functionalunknown genes were also identified. Furthermore,mRNA sequencing expression profiling of O. fragranswere compared to previous genes’. In summary, thiscomprehensive transcriptome dataset allows the identificationof genes associated with several major metabolicpathways and provides a useful public information platformfor further functional genomic studiesin O. fragransLour.
Timosaponin B-Ⅱ Inhibits Pro-inflammatory Cytokine Induction by Lipopolysaccharide in BV2 Cells
Wen-Quan Lu,Wan-Sheng Chen,Yan Qiu,Tie-Jun Li,Xia Tao,Lian-Na Sun 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.9
It was reported that the total polysaccharides extracts from Anemarrhenae asphodeloides Bge(Liliaceae, rhizome) could inhibit inflammatory responses in various models. In the present study, the effects of Timosaponin B-II, a purified extract from A. asphodeloidesb, on the expressions of IL-1β, TNF-α and IL-6, the activity of NF-κB and the activation of signal pathway related to NF-κB were explored in vitro. Timosaponin B-Ⅱ significantly attenuated increase of these cytokines on both mRNA and protein levels from LPS-stimulated BV2 cells in a dose-dependent manner. The reporter gene assay also showed that the activation of NF-κB induced by LPS was inhibited by pre-treatment with Timosaponin B-Ⅱ. Moreover, western blot results showed that the activation of p38, JNK and P65 had been decreased. These results suggest that both NF-κB signal pathway and MAPK pathway were involved in the inhibitory effects of Timosaponin B-II on the expression of pro-inflammatory cytokines.