Plant Regeneration of B.juncea Through Plant Tissue and Protoplast Culture

Lian, Yu-Ji,Lim, Hak-Tae The Korean Society of Plant Biotechnology 2001 Plant molecular biology and biotechnology research Vol.3 No.1

New types of cytoplasmic male sterility in Brassica species would be very useful for the production of F$_1$, hybrid seeds. Leaves and stems of rapid cycling stock of B.juncea (CrGC4-3) containing Anand CMS were used as experimental materials for plant regeneration from protoplast culture. Very high plant regeneration rate (85%) was found in the Kao & Michayluk medium supplemented with 2 mg/L zeatin, 0.5 mg/L BAP, and 1 mg/L NAA when only leaf, not stem, segments were cultured. Protoplasts were isolated from leaves using mixtures of enzymes (1% Cellulycin, 0.5% Macerozyme) in 0.4 M mannitol and 50 mM $CaCl_2$.$2H_2$O. Mcrocalli induced from protoplasts were transferred to the shoot regeneration medium containing 2 mg/L BAP, 2 mg/L zeatin, and 0.5 mg/L NAA. After 60 days of initial protoplast culture, regenerated plantlets were obtained, acclimatized, transplanted into the pots, and grown up to the flowering stage.

Cimiside E Arrests Cell Cycle and Induces Cell Apoptosis in Gastric Cancer Cells

Lian Yu Guo,Eun Ji Joo,손건호,Su Jin Jeon,Sehyun Jang,신은명,Hong Yu Zhou,Yeong Shik Kim 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.10

Cimiside E was isolated from the Cimicifuga heracleifolia Komarov extract, which has been previously demonstrated to possess apoptotic action on gastric cancer cells. The IC50 value of cimiside E on gastric cancer cells for 24 h was 14.58 μM. The mechanism of apoptosis was further elucidated through western blot, RT-PCR, morphology, Annexin V-FITC/PI staining and cell cycle analysis. Cell cycle arrest was induced by cimiside E in S phase at a lower concentration (30 μM) and G2/M phase at higher concentrations (60 and 90 μM). Cimiside E mediated apoptosis through the induction of the caspase cascade for both the extrinsic and intrinsic pathways. These findings suggest that cimiside E may be an effective chemopreventive agent against cancer.

Production and Characterizations of Somatic Hybrids between Brassica campestris L. ssp pekinensis and Brassica of oleracea L. var capitata

Lian, Yu-Ji,Lim, Hak-Tae The Korean Society of Plant Biotechnology 2001 Plant molecular biology and biotechnology research Vol.3 No.1

Protoplasts isolated from inbred lines of Brassica oleracea L. var capitata (cabbage) and Brassica campestris L. ssp. pekinensis (Chinese cabbage) were fused by PEG-mediated method, and somatic hybrid cells were differentiated into plants. for the identification of somatic hybrid plants, ploidy level, plant morphology, and cytological analysis were performed. All of the regenerated plants derived from fused protoplasts were shown to be 2X-4X, or higher ploidy level, presumably due to somatic hybridization or chromosome doubling. The morphology of leaves, petioles, and flowers showed an intermediate phenotype between Chinese cabbage and cabbage. Chromosome numbers in these somatic hybrids ranged mostly from 33 to 38. According to Genomic in situ hybridization (GISH) pattern, signals from both fusion parents of B.campestris or B.oleracea were detected in different colors when chromosomes of putative somatic hybrids were observed.

Comparison of Suppressive Effects of Demethoxycurcumin and Bisdemethoxycurcumin on Expressions of Inflammatory Mediators In Vitro and In Vivo

Lian Yu Guo,Xing Fu Cai,Jung Joon Lee,강삼식,신은명,Hong Yu Zhou,Ji Won Jung,김영식 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.4

Demethoxycurcumin and bisdemethoxycurcumin are the main active ingredients isolated from Curcumae Longae Radix. Recent studies demonstrated that both compounds exhibit antioxidative and anti-inflammatory effects as well as effects on cancer cell lines. In this study, we compared the activities of demethoxycurcumin and bisdemethoxycurcumin, and both compounds were evaluated on lipopolysaccharide (LPS)-induced nitric oxide (NO) production, inducible nitric oxide synthase (iNOS), cycloxygenase-2 (COX-2) and nuclear factor-kappaB (NF-κB) activity in a RAW 264.7 macrophage cell line. The evaluation:results suggested that the anti-inflammatory properties of demethoxycurcumin and bisdemethoxycurcumin were attributed to the inhibition of iNOS and COX-2 expression, as initiated by the inhibition of NF- κB activity. Additionally, both of them significantly inhibited carrageenan-induced paw edema in mice. Taken together, all of the results showed that the suppressive effect of demethoxycurcumin was stronger than that of bisdemethoxycurcumin, indicating that the methoxy group had enhanced demethoxycurcumin’s anti-inflammation effects.

Comparison of Suppressive Effects of Demethoxycurcumin and Bisdemethoxycurcumin on Expressions of Inflammatory Mediators In Vitro and In Vivo

Guo, Lian Yu,Cai, Xing Fu,Lee, Jung-Joon,Kang, Sam-Sik,Shin, Eun-Myoung,Zhou, Hong-Yu,Jung, Ji-Won,Kim, Yeong-Shik 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.4

Demethoxycurcumin and bisdemethoxycurcumin are the main active ingredients isolated from Curcumae Longae Radix. Recent studies demonstrated that both compounds exhibit antioxidative and anti-inflammatory effects as well as effects on cancer cell lines. In this study, we compared the activities of demethoxycurcumin and bisdemethoxycurcumin, and both compounds were evaluated on lipopolysaccharide (LPS)-induced nitric oxide (NO) production, inducible nitric oxide synthase (iNOS), cycloxygenase-2 (COX-2) and nuclear factor-kappaB $(NF-{\kappa}B)$ activity in a RAW 264.7 macrophage cell line. The evaluation:results suggested that the anti-inflammatory properties of demethoxycurcumin and bisdemethoxycurcumin were attributed to the inhibition of iNOS and COX-2 expression, as initiated by the inhibition of $NF-{\kappa}B$ activity. Additionally, both of them significantly inhibited carrageenan-induced paw edema in mice. Taken together, all of the results showed that the suppressive effect of demethoxycurcumin was stronger than that of bisdemethoxycurcumin, indicating that the methoxy group had enhanced demethoxycurcumin's anti-inflammation effects.

MicroRNA-122 Promotes Proliferation, Invasion and Migration of Renal Cell Carcinoma Cells Through the PI3K/Akt Signaling Pathway

Lian, Ji-Hu,Wang, Wei-Hua,Wang, Jia-Qiang,Zhang, Yu-Hong,Li, Yi Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Objective: MicroRNAs (miRNAs) are a small class of non-coding, single-stranded RNAs with a critical role in genesis and maintenance of renal cancer mainly through binding to 3'-untranslated regions (3'UTR) of target mRNAs, which causes a block of translation and/or mRNA degradation. The aim of the present study was to investigate the potential effects of miR-122 in human renal cell carcinomas. Methods: The expression level of miR-122 was quantified by qRT-PCR. MTT, colony formation, invasion and migration assays were used to explore the potential functions of miR-122 in human renal cell carcinoma cells. Results: Cellular growth, invasion and migration in two A498 and 786-O cells were significantly increased after miR-122 transfection. Further experiments demonstrated that overexpression of miR-122 resulted in the increase of phospho-Akt (Ser473) and phospho-mTOR (Ser2448), then activation of mTOR targets, p70S6K and 4E-BP1. Conclusions: The up-regulation of miR-122 may play an important role in the progress of renal cancer through activating PI3K/Akt signal pathway and could be a potential molecular target for anti-cancer therapeutics.

Anti-inflammatory Effects of (Z)-Ligustilide through Suppression of Mitogen-activated Protein Kinases and Nuclear Factor-κB Activation Pathways

Ji Won Chung,김영식,최란주,서은경,Joo-Won Nam,Mi-Sook Dong,Eun Myoung Shin,Lian Yu Guo 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.4

The roots of Angelica tenuissima have been commonly used for the treatment of cardiovascular diseases and menstrual discomfort in Asian countries, such as China and Korea. The primary volatile flavor components are essential oil ingredients, phthalide lactones. In this study, (Z)-ligustilide was tested for its anti-inflammatory activities in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. We found that (Z)-ligustilide strongly inhibitis the induction of LPS-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at both the mRNA and protein levels in a dose-dependent manner. The transcriptional activity of nuclear factor kappa B (NF-κB) was also down-regulated in a concentration-dependent manner. Further study revealed that (Z)-ligustilide inhibited the phosphorylation and subsequent degradation of IκBα, an inhibitor protein of NF-κB. In addition, (Z)-ligustilide inhibited the phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK), extracellular signal-regulated kinase (ERK) and c-Jun NH2-terminal kinase (JNK) in a dose-dependent manner. Taken together, these data suggest that (Z)-ligustilide can exert its antiinflammatory effects by regulating the NF-κB and MAPK signal pathways.

Effects of Schisandrin on Transcriptional Factors in Lipopolysaccharide- Pretreated Macrophages

Lian Yu Guo,Tran Manh Hung,배기환,김영식,Sehyun Jang,신은명,Ji Won Chung,강삼식,김현표 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.3

Schisandrin is the main active ingredient isolated from Schisandra chinensis Baill. Recent studies have demonstrated that schisandrin exhibits anti-inflammatory effects in vivo and in vitro. In this study, we examined whether the order of lipopolysaccharide (LPS) treatment affects the mechanism of schisandrin anti-inflammatory activity. We found that the antiinflammatory mechanisms are not the same depending on whether macrophages were treated with schisandrin before or after LPS. The main difference is that inhibitor kappaBα (IκBα) degradation was not inhibited when macrophages were pretreated by LPS before schisandrin and was weakly inhibited when macrophages were pretreated by schisandrin before LPS.

Nitric oxide synthase inhibitors: a review of patents from 2011 to the present

Yang, Yanyan,Yu, Tao,Lian, Yu-ji,Ma, Rujun,Yang, Sungjae,Cho, Jae Youl Informa UK, Ltd. 2015 Expert opinion on therapeutic patents Vol.25 No.1

<P><B><I>Introduction:</I></B> Nitric oxide synthases (NOSs) are a family of enzymes that play an essential role in synthesizing nitric oxide (NO) by oxidizing <SMALL>L</SMALL>-arginine. As previously reported, NO is a significant mediator in cellular signaling pathways. It serves as a crucial regulator in insulin secretion, vascular tone, peristalsis, angiogenesis, neural development and inflammation. Due to its important role, the inhibition of these vital enzymes provides, as tools, the opportunity to gain an insight into potential therapeutic applications targeting NOSs.</P><P><B><I>Areas covered:</I></B> This paper reviews the patent literature between 2011 and mid-2014 that specified inhibitors of NOS family members as the significant targets. Google and Baidu search engines were used to find relevant patents and clinical information using NOSs or NOS inhibitor as search terms.</P><P><B><I>Expert opinion:</I></B> Considerable recent progress has been made in the development of NOS inhibitors with pharmacodynamic and pharmacokinetic properties, and such development is likely to continue. The patented compounds attenuated mostly embodying evidence from <I>in vitro</I> and <I>in vivo</I> trials that demonstrate good potential for future clinical human trials and industrial applications. Furthermore, new techniques such as X-ray ligand crystallographic study and structure-activity relationship were popularly utilized, which give new insights for developing novel, safe, efficient and selective NOS inhibitors.</P>

Study of the Enhancement of Magnetic Properties of NdFeB Materials Fabricated by Modified HDDR Process

Meng Fu,Fa-zeng Lian,Ji-jie Wang,Wen-li Pei,Yu-lan Chen,Hong-cai Yang 한국자기학회 2004 Journal of Magnetics Vol.9 No.4

The HDDR (Hydrogenation-Disproportionation-Desorption-Recombination) process is a special method to produce anisotropic NdFeB powders for bonded magnet. The effect of the modified HDDR process on magnetic properties of Nd₂Fe₁₄B-based magnet with several composition Nd_(11.2)Fe_(66.5-x)Co_(15.4)B_(6.8)Zr_(0.1)Ga_x (x = 0~1.0) and that of microelement Ga, disproportional temperature and annealing temperature on jHc, grain size were investigated in order to produce anisotropic powder with high magnetic properties. It was found that modified HDDR process is very effective to enhance magnetic properties and to fine grain size. The addition of Ga could change disproportionation character remarkably of the alloy and could improve magnetic properties of magnet powder. Increasing annealing temperature induces significant grain growth. And grain size produced by modified HDDR process is significantly smaller than those produced by conventional HDDR process.