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RISS 인기검색어
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- 저자 : Li Fanchi (검색결과 2 건)
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Study of compensatory growth based on different nutrition conditions of Bombyx mori
Dai Minli,Feng Piao,Mao Tingting,Gu Haoyi,Bian Dandan,Sun Haina,Li Fanchi,Wei Jing,Li Bing 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.3
Organisms achieve compensatory growth after a period of nutrient restriction followed by recovering the nutrient status. However, the underlying mechanisms associated with such growth acceleration remain unclear. The silkworm Bombyx mori is a lepidopteran model insect. This study aimed to investigate the physiological characteristics and the underlying mechanisms in B. mori fed on mulberry leaves (MG), artificial diet (AG), and artificial diet + mulberry leaves (AG-MG), respectively. Silkworms in AG-MG which fed on artificial diet from 1st to 3rd instars followed by feeding on mulberry leaves from 4th to 5th instars exhibited a higher weight gain rate than that in MG and AG, indicating that compensatory growth occurred as a result of the switch in the silkworm food regime. Trypsin and lipase activities of silkworms in AG-MG were shown to be up-regulated at 72 h after changing food. Digital gene expression profiling (DGE) analysis revealed that genes related to metabolism and development in silkworm midguts were differentially expressed. The quantitative real-time PCR (qRT-PCR) re sults showed that the expression levels of IIS/PI3K-AKT pathway genes including INR, IRS, AKT, PI3K60 and PI3K110 of silkworms in AG were down-regulated compared with that in MG at 0 h. Whereas AKT, PI3K and PI3K60 of silkworms were significantly increased by 1.68-, 1.49-, 1.67-fold, respectively, at 72 h after switched to mulberry leaves than the same instar fed on artificial diet. PDK’s expression of silkworms in AG was higher than that in other two groups at each timepoint. Compared with MG and AG, PTEN and IRS were down-regulated at 48 and 72 h in AG-MG. Collectively, these results indicate that compensatory growth in B. mori is regulated by IIS/ PI3K-AKT signaling pathway.
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Effects of different diets on the growth and development of young silkworms
Wang Yuanfei,Shu Qilong,Gu Haoyi,Feng Piao,Dai Minli,Zhu Qingyu,Liu Wei,Dai Yan,Li Fanchi,Li Bing 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.4
To investigate the impact of different diets on the growth and development of the silkworm Bombyx mori (L.; Lepidoptera: Bombycidae), we conducted an analysis of differentially expressed genes (DEGs) between larvae fed an artificial diet (referred to as the AD group) and those fed mulberry leaves (referred to as the ML group) during the first, second, and third instars using RNA sequencing (RNA-Seq) technology. Our results revealed that the DEGs primarily belonged to pathways associated with lipid metabolism, carbohydrate metabolism, amino acid metabolism, the endocrine system, the nervous system, the digestive system, the immune system, and oxida tion–reduction processes. Notably, in the AD group, there was a decrease in transcript levels of genes related to amino acid metabolism synthases, while the transcript levels of antimicrobial peptide genes were up-regulated. Furthermore, genes associated with cytochrome P450 (CYP) enzymes exhibited down-regulation in the AD group. These observed changes likely contributed to the delayed growth and compromised robustness observed in the AD group. Overall, these findings provided valuable insights into the mechanisms underlying the differ ences in growth, development, disease resistance, and adversity resistance between silkworms fed an artificial diet and those fed mulberry leaves.
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