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RISS 인기검색어
- 검색키워드
- 저자 : Leonardo Quintana Soares Lopes (검색결과 2 건)
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Roberto Christ Vianna Santos,Leonardo Quintana Soares Lopes,Camilla Filippi dos Santos Alves,Viviane Pedroso Fausto,Kauana Pizzutti,Victor Barboza,Marcia Ebling de Souza,Renata Platchek Raffin,Patrici 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.3
Paenibacillus larvae and Melissococcus plutonius are the primary bacterial pathogens of honeybees and the causativeagents of American and European foulbrood disease (AFB and EFB) respectively. Such diseases have beengaining importance since there are few therapeutic options beyond the reporting of microorganisms resistantto conventional antibiotics. Due to the inefficiency and/or lowefficacy of some antibiotics, researches with nanotechnologyrepresent, possibly, new therapeutic strategies. Nanostructured drugs have presented some advantagesover the conventional medicines, such as slow, gradual and controlled release, increased bioavailability,and reduced side-effects, among others. In this study, in vitro antimicrobial activity of tea tree oil (TTO) nanoparticlesagainst Paenibacillus species, including P. larvae and M. plutonius strains was evaluated. Minimal inhibitoryconcentration (MIC) in Mueller–Hinton or KSBHI broth by the microdilution method was assessed. TTO registeredMIC values of 0.18–6.25%, while the MIC values obtained for the TTO nanoparticle were of 0.01–0.93%. The possible toxic effect of TTO and TTO nanoparticle has been assessed by the spraying application method inthe concentrations higher than the MICs. Bee mortality was evident only in treatment with TTO and the TTOnanoparticles show no toxic effects after 7 days of observation. Our results showed for the first time that TTOnanoencapsulation presented a high activity against Paenibacillus species and M. plutonius strains showing thatthe use of nanotechnology may represent one alternative way for the treatment or prevention of AFB and EFB.
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Rodrigo de Almeida Vaucher,Janice Luehring Giongo,Leandro Perger Bolzan,Marcos Saldanha Côrrea,Viviane Pedroso Fausto,Camilla Filippi dos Santos Alves,Leonardo Quintana Soares Lopes,Aline Augusti Boli 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.2
Antimicrobial activity of Amazonian oils Andiroba and Copaiba against Paenibacillus larvae has been recently determined, indicating their potential use for the control of American Foulbrood Disease (AFB), but the use of essential oils in the environment still represents a challenge. The oils present several volatile elements in its composition, such high volatility being the cause of a sharp decline in antimicrobial activity. In this context the nanostructuration of these amazon oils may decrease the volatile characteristic of such products. The following research aimed to evaluate the activity of nanoemulsions prepared with Andiroba and Copaiba oils against Paenibacillus species. The toxicity of nanoemulsions has also been investigated with larvae and adult worker bees. Nanoemulsions (NE1, 10% Andiroba oil; NE2, 10% Copaiba oil; and NE3, 10% medium-chain triglyceride as negative control) were prepared in a high pressure homogenizer. The particle sizes were determined as 192, 211, and 178 nm for NE1, NE2, and NE3, respectively. The z potential values were −56.4, −47.1, and −27.2, respectively. NE1 and NE2 showed minimal inhibitory concentration (MIC) values lower than 0.39% for most Paenibacillus species tested. None of the strains were inhibited by negative control NE3. The timeresponse effect of the nanoemulsions has been tested on P. larvae ATCC9545, resulting in a decrease in the number of viable cells to less than 1 log CFU/ml for NE1. The nanoemulsion NE1 showed a significant toxic effect for the larvae (26% mortality) when compared with NE2 (13%) and NE3 (7%). The toxic effect of nanoemulsions has also been evaluated for 72 h in adult worker bees and low mortality rate was only observed for the NE1 treatment (8.3%). This study shows for the first time that nanoemulsions of Copaiba oil can be a potential candidate for the treatment or prevention of AFB.
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