A rapid antimicrobial susceptibility test based on single-cell morphological analysis

Choi, Jungil,Yoo, Jungheon,Lee, Mincheol,Kim, Eun-Geun,Lee, Ji Soo,Lee, Seungok,Joo, Seik,Song, Sang Hoon,Kim, Eui-Chong,Lee, Jung Chan,Kim, Hee Chan,Jung, Yong-Gyun,Kwon, Sunghoon American Association for the Advancement of Scienc 2014 Science Translational Medicine Vol.6 No.267

<P>A rapid antibiotic susceptibility test (AST) is desperately needed in clinical settings for fast and appropriate antibiotic administration. Traditional ASTs, which rely on cell culture, are not suitable for urgent cases of bacterial infection and antibiotic resistance owing to their relatively long test times. We describe a novel AST called single-cell morphological analysis (SCMA) that can determine antimicrobial susceptibility by automatically analyzing and categorizing morphological changes in single bacterial cells under various antimicrobial conditions. The SCMA was tested with four Clinical and Laboratory Standards Institute standard bacterial strains and 189 clinical samples, including extended-spectrum β-lactamase–positive <I>Escherichia coli and Klebsiella pneumoniae</I>, imipenem-resistant <I>Pseudomonas aeruginosa</I>, methicillin-resistant <I>Staphylococcus aureus</I>, and vancomycin-resistant <I>Enterococci</I> from hospitals. The results were compared with the gold standard broth microdilution test. The SCMA results were obtained in less than 4 hours, with 91.5% categorical agreement and 6.51% minor, 2.56% major, and 1.49% very major discrepancies. Thus, SCMA provides rapid and accurate antimicrobial susceptibility data that satisfy the recommended performance of the U.S. Food and Drug Administration.</P>

Inhibition of Oral Epithelial Cell Growth in vitro by Epigallocatechin-3-gallate

Jungil Hong,Mi-Ri Kim,Na Hyun Lee,Bo Hyun Lee 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.4

The most abundant tea catechin, epigallocatechin-3-gallate (EGCG), has been reported to inhibit cell proliferation and induce apoptosis in many types of cancer cells. In the present study, effects of EGCG on the growth of oral epithelial cells including CAL-27 oral squamous carcinoma cells and dysplastic oral keratinocytes (DOK) were investigated. EGCG inhibited growth of CAL-27 cells and DOK with IC?? of 14.4-21.0 and 5.8-14.2 μM after 24 and 48 hr incubation, respectively. EGCG was significantly less effective in inhibiting DOK growth. The effects of EGCG, however, were dramatically less pronounced in the presence of superoxide dismutase (SOD) and catalase. Inhibitory effects of EGCG on CAL-27 cell growth were also much less pronounced in the presence of fetal bovine serum (FBS). EGCG induced caspase-3 activation in both CAL-27 and DOK cells in a serum free condition without SOD/catalase; in the presence of 10% FBS and SOD/catalase, EGCG, even at 100 μM, did not affect cell growth. The present results indicate that EGCG inhibited oral cell growth with higher potency to more malignant CAL-27 cells than DOK, and the effects were markedly altered by SOD/catalase and serum content in media.

QCD Radiative Corrections to J/ψ Leptonic Decay by Using the Scalar-Integral Reduction Method

Jungil Lee,Chaehyun Yu 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.49 No.4

We study the scalar-integral reduction method, which uses the integration-by-parts method, in calculating s corrections to heavy-quarkonium amplitudes. We apply this method to leptonic decay of the spin-triplet S-wave heavy quarkonium as an illustrative example. All possible scalar integrals are reduced into a single master integral in the J=A ! `+`¡ decay at this order. One drawback of this method is that one cannot avoid a mixture of ultraviolet (UV) and infrared (IR) poles during the reduction. We show a way to classify the origin of divergences in loop integrals in the scalar-integral reduction method. We expect this method to be useful in constructing a fast algebraic algorithm for QCD radiative corrections to quarkonium processes.

TMEM165, a Golgi transmembrane protein, is a novel marker for hepatocellular carcinoma and its depletion impairs invasion activity

Lee, Jee-San,Kim, Mi-Yeun,Park, Eun-Ran,Shen, Yan Nan,Jeon, Ju-Yeon,Cho, Eung-Ho,Park, Sun-Hoo,Han, Chul Ju,Choi, Dong Wook,Jang, Ja June,Suh, Kyung-Suk,Hong, Jungil,Kim, Sang Bum,Lee, Kee-Ho D.A. Spandidos 2018 Oncology Reports Vol.40 No.3

<P>Transmembrane protein 165 (TMEM165), a Golgi protein, functions in ion homeostasis and vesicular trafficking in the Golgi apparatus. While mutations in <I>TMEM165</I> are known to cause human ‘congenital disorders of glycosylation’, a recessive autosomal metabolic disease, the potential association of this protein with human cancer development has not been explored to date. In the present study, we revealed that <I>TMEM165</I> is overexpressed in HCC and its depletion weakens the invasive activity of cancer cells through suppression of matrix metalloproteinase-2 (MMP-2) expression. Levels of <I>TMEM165</I> mRNA and protein were clearly increased in HCC patient tissues and cell cultures. Quantitative real-time RT-PCR analysis of fresh HCC tissues (n=88) revealed association of <I>TMEM165</I> overexpression with more frequent macroscopic vascular invasion, microscopic serosal invasion and higher α-fetoprotein levels. Notably, depletion of <I>TMEM165</I> led to a marked decrease in the invasive activity of two different HCC cell types, Huh7 and SNU475, accompanied by downregulation of MMP-2. Our collective findings clearly indicated that TMEM165 contributed to the progression of HCC by promoting invasive activity, supporting its utility as a novel biomarker and therapeutic target for cancer.</P>

Reference X-ray Irradiation System for Personal Dosimeter Testing and Calibration of Radiation Detector

Lee, Seung Kyu,Chang, Insu,Kim, Sang In,Lee, Jungil,Kim, Hyoungtaek,Kim, Jang-Lyul,Kim, Min Chae The Korean Association for Radiation Protection 2019 방사선방어학회지 Vol.44 No.2

Background: In the calibration and testing laboratory of Korea Atomic Energy Research Institute, the old X-ray generator used for the production of reference X-ray fields was replaced with a new one. For this newly installed X-ray irradiation system, beam alignment as well as the verification of beam qualities was conducted. Materials and Methods: The existing X-ray generator, Phillips MG325, was replaced with YXLON Y.TU 320-D03 in order to generate reference X-ray fields. Theoretical calculations and Monte Carlo simulations were used to determine initial filter thickness. Beam alignment was performed in three steps to deliver a homogeneous radiation dosage to the target at different distances. Finally, the half-value layers were measured for different X-ray fields to verify beam qualities by using an ion chamber. Results and Discussion: Beam alignment was performed in three steps, and collimators and other components were arranged to maintain the uniformity of the mean air kerma rate within ${\pm}2.5%$ at the effective beam diameter of 28 cm. The beam quality was verified by using half-value layer measurement methods specified by American National Standard Institute (ANSI) N13.11-2009 and International Organization for Standardization (ISO)-4037. For each of the nine beams than can be generated by the new X-ray irradiation system, air kerma rates for X-ray fields of different beam qualifies were measured. The results showed that each air kerma rate and homogeneity coefficient of the first and second half-value layers were within ${\pm}5%$ of the recommended values in the standard documents. Conclusion: The results showed that the new X-ray irradiation system provides beam qualities that are as high as moderate beam qualities offered by National Institute of Standards and Technology in ANSI N13.11-2009 and those for narrow-spectrum series of ISO-4037.

<i>OsCOL4</i> is a constitutive flowering repressor upstream of <i>Ehd1</i> and downstream of <i>OsphyB</i>

Lee, Yang-Seok,Jeong, Dong-Hoon,Lee, Dong-Yeon,Yi, Jakyung,Ryu, Choong-Hwan,Kim, Song L.,Jeong, Hee J.,Choi, Sang C.,Jin, Ping,Yang, Jungil,Cho, Lae-Hyeon,Choi, Heebak,An, Gynheung Blackwell Publishing Ltd 2010 The Plant journal Vol.63 No.1

<P>Summary</P><P>Plants recognize environmental factors to determine flowering time. <I>CONSTANS</I> (<I>CO</I>) plays a central role in the photoperiod flowering pathway of Arabidopsis, and CO protein stability is modulated by photoreceptors. In rice, <I>Hd1</I>, an ortholog of <I>CO</I>, acts as a flowering promoter, and phytochromes repress <I>Hd1</I> expression. Here, we investigated the functioning of <I>OsCOL4</I>, a member of the <I>CONSTANS-like</I> (<I>COL</I>) family in rice. <I>OsCOL4</I> null mutants flowered early under short or long days. In contrast, <I>OsCOL4</I> activation-tagging mutants (<I>OsCOL4-D</I>) flowered late in either environment. Transcripts of <I>Ehd1</I>, <I>Hd3a</I>, and <I>RFT1</I> were increased in the <I>oscol4</I> mutants, but reduced in the <I>OsCOL4-D</I> mutants. This finding indicates that <I>OsCOL4</I> is a constitutive repressor functioning upstream of <I>Ehd1.</I> By comparison, levels of <I>Hd1</I>, <I>OsID1</I>, <I>OsMADS50</I>, <I>OsMADS51</I>, and <I>OsMADS56</I> transcripts were not significantly changed in <I>oscol4</I> or <I>OsCOL4-D</I>, suggesting that <I>OsCOL4</I> functions independently from previously reported flowering pathways. In <I>osphyB</I> mutants, <I>OsCOL4</I> expression was decreased and <I>osphyB oscol4</I> double mutants flowered at the same time as the <I>osphyB</I> single mutants, indicating <I>OsCOL4</I> functions downstream of <I>OsphyB.</I> We also present evidence for two independent pathways through which OsPhyB controls flowering time. These pathways are: (i) night break-sensitive, which does not need <I>OsCOL4</I>; and (ii) night break-insensitive, in which <I>OsCOL4</I> functions between <I>OsphyB</I> and <I>Ehd1.</I></P>

Nullifying Tumor Efflux by Prolonged Endolysosome Vesicles: Development of Low Dose Anticancer-Carbon Nanotube Drug

Lee, Yeon Kyung,Choi, Jungil,Wang, Wenping,Lee, Soyoung,Nam, Tae-Hyun,Choi, Wan Sung,Kim, Chang-Joon,Lee, Jong Kwon,Kim, Sang-Hyun,Kang, Sang Soo,Khang, Dongwoo American Chemical Society 2013 ACS NANO Vol.7 No.10

<P>As the majority of side effects of current chemotherapies stems from toxicity due to excessive dosing of anticancer drugs, minimizing the amount of drug while maximizing drug efficacy is essential to increase the life-quality of chemotherapy patients. This study demonstrated that the intracellular delivery of amide linked doxorubicin on carbon nanotube can nullify the efflux of cancer cells by achieving prolonged endolysosome delivery and can induce burst release of doxorubicin in an acidic hydrolase environment and, ultimately, can reduce the amount of anticancer drug by 10-fold compared to conventional effective drug dose. The clearance of accumulated carbon nanotubes in the liver was observed after 4 weeks, and analysis of liver toxicity markers showed no significant changes in GOT and GPT levels and release of pro-inflammatory cytokines across both short- and long-term periods.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/ancac3/2013/ancac3.2013.7.issue-10/nn4041206/production/images/medium/nn-2013-041206_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nn4041206'>ACS Electronic Supporting Info</A></P>

Relativistic corrections to the axial vector and vector currents in the $ \bar{b}c $ meson system at order α s

Lee, Jungil,Sang, WenLong,Kim, Seyong Springer-Verlag 2011 Journal of high energy physics Vol.2011 No.1

One-Loop Master Integral for Order-v2n Relativistic Corrections Γ[J/ψ→e+e-]

Jungil Lee,Chaehyun Yu,Hyeon-Kyun Noh 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.50 No.2

We show that one-loop scalar integrals contributing to the order-$v^{2n}$ relativistic corrections to the QCD vertex corrections to the spin-triplet $S$-wave heavy-quark-antiquark pair annihilation into a virtual photon can be expressed in terms of a single master integral. With an algebraic reduction combined with the tensor-integral reduction method, we first simplify the scalar integrals as linear combinations of two-point functions. The two-point functions are reduced into a single master integral by using the recurrence relations derived from integration by parts. Order-$v^2$ formulas are explicitly derived, and a strategy to calculate the higher-order relativistic corrections at order $\alpha_s$ is also given. The result may be very useful in calculating the order-$v^{2n}$ relativistic corrections to the leptonic decay width of the spin-triplet $S$-wave heavy quarkonium at order $\alpha_s$.

[논문 발표문 3] 연구 제목을 통해 살펴본 스포츠 코칭 연구 동향 분석

이준길(Jungil Lee) 한국융합코칭학회 2022 한국융합코칭학회 학술대회자료집 Vol.2022 No.5

본 연구는 탐색적 연구로써 스포츠 코칭의 연구 제목을 통해 연구 동향을 살펴보기 위한 기초 작업으로 볼 수 있다. 본 연구 결과를 요약하면 다음과 같다. 2015년부터 2020년까지 총 26개의 학회에서 논문이 등록되었으며, 66개의 논문과 25개의 연구 주제, 다양한 연구유형을 살펴볼 수 있다. 다양한 주제와 학회에서 연구가 진행되었으며, 연구 종목에는 태권도(8)와 축구(6)가 가장 많았다. 코칭과 관련된 주제를 살펴보면 ‘코칭행동’(18)과 ‘멘탈코칭’(6)에 대한 연구가 진행되었다. 연구유형에 있어서 ‘체계 정립’, ‘코칭 분류’, ‘코칭 개념’ 연구의 접근이 필요함을 알 수 있었다. 연구 제목을 통해 분류되지 않는 영역, 새로운 영역에 대한 체계 정립 또한 필요하다고 생각된다. 마지막으로 스포츠 코칭 동향 연구에 필요한 코칭개념, 코칭분류를 중점으로 진행되어야 할 것이다. As an exploratory study, this study can be seen as a basic work to examine research trends through the research title of sports coaching. The summary of the results of this study is as follows. From 2015 to 2020, papers were registered at a total of 26 academic societies, and 66 papers, 25 research topics, and various research types could be examined. Research has been conducted in various academic societies and fields as well as various subjects.