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Cand1 Promotes Assembly of New SCF Complexes through Dynamic Exchange of F Box Proteins
Pierce, Nathan W.,Lee, J.,Liu, X.,Sweredoski, Michael J.,Graham, Robert L.J.,Larimore, Elizabeth A.,Rome, M.,Zheng, N.,Clurman, Bruce E.,Hess, S.,Shan, S.o.,Deshaies, Raymond J. Cell Press ; MIT Press 2013 Cell Vol.153 No.1
The modular SCF (Skp1, cullin, and F box) ubiquitin ligases feature a large family of F box protein substrate receptors that enable recognition of diverse targets. However, how the repertoire of SCF complexes is sustained remains unclear. Real-time measurements of formation and disassembly indicate that SCF<SUP>Fbxw7</SUP> is extraordinarily stable, but, in the Nedd8-deconjugated state, the cullin-binding protein Cand1 augments its dissociation by one-million-fold. Binding and ubiquitylation assays show that Cand1 is a protein exchange factor that accelerates the rate at which Cul1-Rbx1 equilibrates with multiple F box protein-Skp1 modules. Depletion of Cand1 from cells impedes recruitment of new F box proteins to pre-existing Cul1 and profoundly alters the cellular landscape of SCF complexes. We suggest that catalyzed protein exchange may be a general feature of dynamic macromolecular machines and propose a hypothesis for how substrates, Nedd8, and Cand1 collaborate to regulate the cellular repertoire of SCF complexes.