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- 저자 : Largia Muthiah Joe Virgin (검색결과 2 건)
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Largia Muthiah Joe Virgin,Pandian Subramani,Shilpha Jayabalan,Chitradevi Muniyarajan,Kavikkuil Manickam,Sohn Soo In,Ramesh Manikandan 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.6
Justicia gendarussa Burm. f. is an indispensable medicinal plant owing to its abundance of phytoconstituents and medicinal uses. In the study, we demonstrated the successful in vitro regeneration of J. gendarussa through nodal explants and its genetic and phytochemical analysis. A combination of 2 mg L-1 BA + 0.1 mg L-1 NAA + 15 mg L-1 spermidine was found to be reliable in producing the maximum number of multiple shoots (16.6) and vigorous roots in Murashige and Skoog medium. Rooted shoots have been efciently hardened and then slowly acclimatized to natural conditions. SPAR-based genetic fdelity checking revealed the true-to-type nature of in vitro regenerated plants with little variations by generating 105 bands through amplifcation of 15 primers. Furthermore, the protocol followed for in vitro culture was strengthened by the comparative determination of Total Phenolic Content (TPC), Total Flavonoid Content (TFC), and Antioxidant Potential (AP). Methanolic extracts of in vitro plants showed higher amounts of TPC, TFC, and AP when compared to in vivo plants which might be due to the infuence of plant growth hormones. Thus the in vitro regeneration protocol derived would vitally expedite large scale production of true to type plantlets of J. gendarussa. In addition, an attempt was made for the frst time to devise a suitable protocol for the induction of hairy roots from this plant using two Agrobacterium rhizogenes strains which would lead to enhanced production of important anti-HIV metabolites of the plant such as gendarussin A, B and so on.
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Shilpha Jayabalan,Pandian Subramani,Largia Muthiah Joe Virgin,Sohn Soo In,Ramesh Manikandan 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.5
The current study demonstrates an effective protocol for encapsulation of in vitro nodal segments of Solanum trilobatum L. for short-term storage. In a modified Murashige and Skoog medium supplemented with 8.8 µM 6-benzyl adenine (BA), 0.5 µM indole-3-acetic acid (IAA), and 25 mM ammonium nitrate, a gelling matrix of 3% sodium alginate and 80 mM CaCl2.2H2O promoted the highest shoot regeneration frequency (98.4%). The effect of storage temperature, sucrose concentration, and storage time interval on shoot regrowth efficiency was assessed. Synthetic seeds maintained at culture room temperature (25 °C) were shown to be more competent than low-temperature storage (8 °C and 4 °C), with better regeneration efficiency (82.2%). The alginate matrix added with 1.5% sucrose increased the storage potential of synthetic seeds up to 12 weeks with a regeneration frequency of 57.7%. Using start codon-targeted polymorphic (SCoT) markers, the genetic fidelity of plants regrown after 12 weeks of storage was assessed. The SCoT fingerprinting approach has confirmed the post-storage genetic stability of recovered plants with their in vitro source. The present investigation is the first report on the development of synthetic seeds of S. trilobatum L., which would greatly facilitate germplasm conservation and exchange of this invaluable medicinal plant.
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