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Carbohydrate-containing Triton X-100 analogues for membrane protein solubilization and stabilization
Chae, Pil Seok,Wander, Marc J.,Cho, Kyung Ho,Laible, Philip D.,Gellman, Samuel H. The Royal Society of Chemistry 2013 Molecular bioSystems Vol.9 No.4
<P>Membrane protein manipulation is a challenging task owing to limited tertiary and quaternary structural stability once the protein has been removed from a lipid bilayer. Such instability can be overcome by embedding membrane proteins in detergent micelles formed from amphiphiles with carefully tuned properties. This study introduces a class of easy-to-synthesize amphiphiles, which are designated CGT (Chae's Glyco-Triton) detergents. Some of the agents are well suited for membrane protein solubilization and stabilization.</P> <P>Graphic Abstract</P><P>This study introduces a class of easy-to-synthesize amphiphiles that display favorable behaviour for membrane protein solubilization and stabilization. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3mb25584k'> </P>